Oxidative stress in deep scattering layers: Heat shock response and antioxidant enzymes activities of myctophid fishes thriving in oxygen minimum zones

2013 ◽  
Vol 82 ◽  
pp. 10-16 ◽  
Author(s):  
Ana Rita Lopes ◽  
Katja Trübenbach ◽  
Tatiana Teixeira ◽  
Vanessa M. Lopes ◽  
Vanessa Pires ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Oxygen Minimum Zones ◽  
Enzymes Activities ◽  
Shock Response ◽  
Antioxidant Enzymes Activities ◽  
Oxygen Minimum

scholarly journals Se Alleviated Oxidative Stress-Mediated Complex Poisoning Mechanism in Pb-Treated Chicken Kidneys: Inflammation, Heat Shock Response, and Autophagy

2021 ◽  
Author(s):  
Zhiying Miao ◽  
Weikang Yu ◽  
Yueyang Wang ◽  
Xianhong Gu ◽  
Xiaohua Teng
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Protein Expression ◽  
Heat Shock Response ◽  
Potable Water ◽  
Histological Structure ◽  
Standard Diet ◽  
Shock Response ◽  
Mrna And Protein Expression ◽  
Shock Proteins

Abstract Background: Lead (Pb) is a toxic environmental pollutant and can exerts toxicity in kidneys. It is known that selenium (Se) has an antagonistic effect on Pb poisoning. However, biological events during the process were not well understood in chicken kidneys.Methods: One hundred and eighty male Hyline chickens (7-day-old) were randomly divided into the control group (offering standard diet and potable water), the Se group (offering Na2SeO3-added standard diet and potable water), the Pb group (offering standard diet and (CH3OO)2Pb-added potable water), and the Pb+Se group (offering Na2SeO3-added standard diet and (CH3OO)2Pb-added potable water). On 30th, 60th, and 90th days, kidneys were removed to perform the studies of histological structure, oxidative stress indicators, cytokines, heat shock proteins, and autophagy in the chicken kidneys.Results: The experimental results indicated that Pb poisoning changed renal histological structure; decreased catalase, glutathione-s-transferase, and total antioxidative capacity activities; increased hydrogen peroxide content; induced mRNA and protein expression of heat shock proteins; inhibited interleukin (IL)-2 mRNA expression, and induced IL-4 and IL-12β mRNA expression; inhibited mammalian target of rapamycin mRNA and protein expression, and induced autophagy-related gene mRNA and protein expression in the chicken kidneys. Supplement of Se mitigated the above changes caused by Pb.Conclusion: Our research strengthens the evidence that Pb induced oxidative stress, inflammation, heat shock response, and autophagy and Se administration alleviated Pb poisoning through mitigating oxidative stress in the chicken kidneys.

Proteins containing non-native disulfide bonds generated by oxidative stress can act as signals for the induction of the heat shock response

1997 ◽  
Vol 171 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Alice T. McDuffee ◽  
Guillermo Senisterra ◽  
Steven Huntley ◽  
James R. Lepock ◽  
Konjeti R. Sekhar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Disulfide Bonds ◽  
Shock Response

scholarly journals Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 157 ◽  
Author(s):  
Shahid Rajput ◽  
Cong Zhang ◽  
Yue Feng ◽  
Xiao Wei ◽  
Mahmoud Khalil ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Basal Diet ◽  
Mitochondrial Pathway ◽  
Bursa Of Fabricius ◽  
Enzymes Activities ◽  
Study Results ◽  
Mrna And Protein Expression ◽  
Antioxidant Enzymes Activities

Aflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopathology, oxidative stress, and apoptosis via the mitochondrial pathway in the bursa of Fabricius (BF) of broilers. One hundred forty-four one-day old Cobb chicks were randomly assigned into four treatment groups of six replicates (6 birds each replicate) for 28 days. Groups were fed on the following four diets; (1) Basal diet without addition of PCs or AFB1 (Control); (2) basal diet supplemented with 1 mg/kg AFB1 from contaminated corn (AFB1); (3) basal diet supplemented with 250 mg/kg PCs (PCs); and (4) basal diet supplemented with 1 mg/kg AFB1 + 250 mg/kg PCs (AFB1+ PCs). The present study results showed that antioxidant enzymes activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) in AFB1 treated group were (p < 0.05) decreased, whereas malondialdehyde (MDA) contents were significantly increased in comparison with the control group. Furthermore, we found that dietary PCs treatment ameliorated AFB1-induced oxidative stress in the BF through inhibiting the accumulation of MDA content and enhancing the antioxidant enzymes activities (T-SOD, CAT, GSH-Px, and GST). Similarly, PCs markedly enhanced messenger RNA (mRNA) expression of antioxidant genes (SOD, CAT, GPx1, and GST) in comparison with AFB1 group. Moreover, histological results showed that PCs alleviated AFB1-induced apoptotic cells in the BF of broilers. In addition, both mRNA and protein expression results manifested that mitochondrial-apoptosis-associated genes (Bax, caspase-9, caspase-3, and p53 and cytochrome c) showed up-regulation, while (Bcl-2) showed down-regulation in AFB1 fed group. The supplementation of PCs to AFB1 diet significantly reversed the mRNA and protein expression of these apoptosis-associated genes, as compared to the AFB1 group. Our results demonstrated that PCs ameliorated AFB1-induced oxidative stress by modulating the antioxidant defense system and apoptosis in the BF through mitochondrial pathway in broilers.

scholarly journals Fresh Bitter Melon Fruit (Momordica charantia) Attenuated Oxidative Stress, Fibrosis and Renal Injury in Carbon Tetrachloride Treated Rats

2018 ◽  
Vol 16 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Md Abu Taher Sagor ◽  
Hasan Mahmud Reza ◽  
Nabila Tabassum ◽  
Md Moshfequr Rahman ◽  
Md Ashraful Alam
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Carbon Tetrachloride ◽  
Renal Disease ◽  
End Stage Renal Disease ◽  
Immune Cell ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities ◽  
Stage Renal Disease ◽  
End Stage

Chemical or drug-induced kidney damage is increasing every year and the end-stage renal disease is becoming a burden for health care system of many countries. Oxidative stress may be a crucial pathway for the development of end-stage renal disease. Thus, natural antioxidant or plant-based therapy would be a better alternative to protect renal function against chemical-induced renal damage. To determine these aspects we evaluated renoprotective effects of M. charantia in carbon tetrachloride administered rats. A 10% w/w mixture of fresh fruits of M. charantia was given with the chow food every day to CCl4 treated rats. After fourteen days, all animals were sacrificed and the kidneys were examined to observe the possible protective effects of M. charantia against CCl4 induced toxicity. The CCl4 treated rats showed increased oxidative stress parameters and decreased antioxidant enzymes activities. Supplementation of 10% w/w M. charantia fruits in CCl4 administered rats prevented the oxidative stress and restored the antioxidant enzymes activities. M. charantia fruits supplementation also prevented the rise of uric acid and creatinine concentration in plasma of CCl4 treated rats. Furthermore, histological studies showed that supplementation of 10% w/w M. charantia fruits prevented the collagen deposition, immune cell migration and iron deposition in kidney sections of CCl4 treated rats. The results of this study revealed that the fruits of M. charantia may protect oxidative stress-mediated damage in kidneys due to CCl4 administration, which is mediated probably via the restoration of anti-oxidant enzyme functions.Dhaka Univ. J. Pharm. Sci. 16(2): 205-214, 2017 (December)

scholarly journals Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response

2016 ◽  
Vol 291 (13) ◽  
pp. 6967-6981 ◽  
Author(s):  
Hong Zhang ◽  
Jie Yang ◽  
Si Wu ◽  
Weibin Gong ◽  
Chang Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Chaperone Dnak ◽  
Shock Response ◽  
Hsp70 Chaperone

P–086 High level of sperm DNA breaks in infertile men with varicocele: its association with sperm cells death, seminal oxidative stress, and spermatic parameters

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
O Ammar ◽  
M Mehdi
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Dna Fragmentation ◽  
Semen Parameters ◽  
Dna Breaks ◽  
Enzymes Activities ◽  
Sperm Dna ◽  
Antioxidant Enzymes Activities ◽  
High Level ◽  
And Oxidative Stress

Abstract Study question Our objectives were to determine the extent of nuclear sperm injury in varicocele patients with and without altered spermatic parameters and to investigate its relationship with apoptosis and oxidative stress. Summary answer Oxidative stress (OS) in the varicocele patients may play a role in the etiology of nuclear sperm DNA damage associated with apoptosis. What is known already Varicocele is associated with high level of DNA Breaks. Study design, size, duration Ejaculated sperm samples from 51 patients diagnosed with varicocele and 29 fertile men were examined. According to the guidelines, the patient’s sperm samples were classified into varicocele with normal semen parameters (n = 11) and varicocele with abnormal semen parameters (n = 40). Participants/materials, setting, methods Sperm DNA breaks was assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The proportion of both viable and dead spermatozoa with externalized phosphatidylserine was detected by the bivariate annexin V cy3/6-CFDA staining method. Seminal malondialdehyde (MDA) amounts and antioxidant enzymes activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured spectrophotometrically. Main results and the role of chance Sperm DNA Breaks, viable spermatozoa with externalized PS, and MDA levels were significantly higher in studied subgroups of patients with varicocele, either with normal or with abnormal semen parameters than controls. The seminal antioxidant enzymes activities were significantly reduced in both subgroups of patients with varicocele compared to the controls. The percentage of spermatozoa with fragmented DNA was positively correlated to the MDA level as well as the proportion of viable spermatozoa with externalized PS. However, the decreased seminal antioxidant status was negatively correlated with the increased proportion of sperm DNA fragmentation and apoptotic spermatozoa. Limitations, reasons for caution We suggest further comparative studies connecting the varicocele patients with and without altered spermatic parameters representing high level of DNA fragmentation with more apoptotic and oxidative stress markers. Wider implications of the findings: This study reveals that impaired seminal antioxidant profile and increased seminal level of lipid peroxidation may be involved in the pathophysiological mechanisms of cell death-mediated DNA breaks in patients with varicocele. Trial registration number Not applicable

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