scholarly journals Inhibition of human matriptase by eglin c variants

FEBS Letters ◽  
2006 ◽  
Vol 580 (9) ◽  
pp. 2227-2232 ◽  
Author(s):  
Antoine Désilets ◽  
Jean-Michel Longpré ◽  
Marie-Ève Beaulieu ◽  
Richard Leduc
Keyword(s):  
Eglin C

Crystal and molecular structure of the bovine α-chymotrypsin-eglin c complex at 2.0 Å resolution

1992 ◽  
Vol 225 (1) ◽  
pp. 107-123 ◽  
Author(s):  
Francesco Frigerio ◽  
Alessandro Coda ◽  
Luisa Pugliese ◽  
Claudia Lionetti ◽  
Enea Menegatti ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Crystal And Molecular Structure ◽  
Eglin C ◽  
C Complex

Complex between the subtilisin from a mesophilic bacterium and the Leech inhibitor eglin-C

1991 ◽  
Vol 47 (5) ◽  
pp. 707-730 ◽  
Author(s):  
Z. Dauter ◽  
C. Betzel ◽  
N. Genov ◽  
N. Pipon ◽  
K. S. Wilson
Keyword(s):  
Eglin C ◽  
Mesophilic Bacterium

Changing the inhibitory specificity and function of the proteinase inhibitor eglin c by site-directed mutagenesis: functional and structural investigation

Biochemistry ◽  
1992 ◽  
Vol 31 (37) ◽  
pp. 8755-8766 ◽  
Author(s):  
Dirk W. Heinz ◽  
Sven G. Hyberts ◽  
Jeff W. Peng ◽  
John P. Priestle ◽  
Gerhard Wagner ◽  
...  
Keyword(s):  
Proteinase Inhibitor ◽  
Structural Investigation ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Inhibitory Specificity ◽  
Eglin C ◽  
And Function

Effect of a Polyhistidine Terminal Extension on Eglin c Stability

1998 ◽  
Vol 263 (1) ◽  
pp. 116-118 ◽  
Author(s):  
Jennifer C. Waldner ◽  
Steven J. Lahr ◽  
Marshall Hall Edgell ◽  
Gary J. Pielak
Keyword(s):  
Eglin C

ICAM-1-CD18 interaction mediates neutrophil cytotoxicity through protease release

1998 ◽  
Vol 274 (6) ◽  
pp. C1634-C1644 ◽  
Author(s):  
Carlton C. Barnett ◽  
Ernest E. Moore ◽  
Gary W. Mierau ◽  
David A. Partrick ◽  
Walter L. Biffl ◽  
...  
Keyword(s):  
Cho Cells ◽  
Chinese Hamster ◽  
Cell Lysis ◽  
Polymorphonuclear Neutrophil ◽  
Intercellular Adhesion Molecule ◽  
Wild Type ◽  
Myristate Acetate ◽  
Intercellular Adhesion Molecule 1 ◽  
Eglin C

Interaction of the β2-integrin complex on the polymorphonuclear neutrophil (PMN) with intercellular adhesion molecule-1 (ICAM-1) has been implicated in PMN-mediated cytotoxicity. This study examined interaction of the CD11a, CD11b, and CD18 subunits of the β2-integrin with ICAM-1, transfected into Chinese hamster ovarian (CHO) cells to avoid effects of other adhesion molecules. Incubation of quiescent PMNs with wild-type and ICAM-1-transfected CHO cells produced nominal cell lysis. Similarly, when phorbol myristate acetate (PMA)-activated PMNs were incubated with wild-type CHO cells, minimal cytotoxicity was produced. However, when ICAM-1-transfected CHO cells were incubated with PMA-activated PMNs, 40% cell lysis occurred. Blockade with a monoclonal antibody (MAb) to ICAM-1 or MAbs to CD11a, CD11b, or CD18 reduced PMN-mediated cytotoxicity to baseline. To examine the role of adhesion in cytotoxicity, we studied β2-integrin-mediated PMN adhesion to ICAM-1-transfected CHO cells and found that MAbs for CD11a, CD11b, and CD18 all abrogated PMN cytotoxicity despite disparate effects on adhesion. To assess the role of CD18, β2-integrin subunits were cross-linked, and CD18 alone mediated protease release. Moreover, ICAM-1 was immunoprecipitated from transfected CHO cells and incubated with PMNs. This soluble ICAM-1 provoked elastase release, similar to PMA, which could be inhibited by MAbs to CD18 but not MAbs to other β2-integrin subunits. In addition, coincubation with protease inhibitors eglin C and AAPVCK reduced PMN-mediated cytotoxicity to control levels. Finally, ICAM-1-transfected CHO cells were exposed to activated PMNs from a patient with chronic granulomatous disease that caused significant cell lysis, equivalent to that of PMNs from normal donors. Collectively, these data suggest that ICAM-1 provokes PMN-mediated cytotoxicity via CD18-mediated protease release.

Computer Simulations of Nonlinear Optical Chromophore Containing Polypeptides

1993 ◽  
Vol 330 ◽  
Author(s):  
Ruth Pachter ◽  
Steven B. Fairchild ◽  
James A. Lupo ◽  
Brian S. Sennett ◽  
Robert L. Crane ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Structure Prediction ◽  
Nonlinear Optical ◽  
Integrated Approach ◽  
Genetically Engineered ◽  
Spatial Proximity ◽  
Tertiary Structures ◽  
Eglin C ◽  
Nonlinear Optical Chromophores ◽  
Dynamics Simulations

ABSTRACTIn our continuing efforts towards the design of nonlinear (NLO) optical chromophore containing polypeptides we present an integrated computational approach, in which the design of biomolecular materials with defined secondary and tertiary structures is investigated by means of novel predictive tools, while the effects of the nonlinear optical chromophores are studied with molecular dynamics simulations. A neural network that was trained to predict the spatial proximity of Cα atoms that are less than a given threshold apart, is applied. The double-iterated Kalman filter (DIKF) technique is then employed with a constraints set that includes these pairwise atomic distances, and the distances and angles that define the structure as it is known from the protein's sequence. The results for test cases, particularly Crambin and genetically engineered Eglin-C, demonstrate that this integrated approach is useful for structure prediction at an intermediate resolution. Defined structural motifs of NLO chromophore containing polypeptides are investigated by using molecular dynamics techniques, particularly for the design of coil coiled amphiphatic biopolymers.

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