BENEFIT OF SHORTER ABSTINENCE PERIOD ON SPERM DNA INTEGRITY IN CASE OF HIGH LEVEL OF OXIDATIVE STRESS

2021 ◽  
Vol 116 (3) ◽  
pp. e347
Author(s):  
Mariem Ben Khelifa ◽  
Sonia Mnallah ◽  
Mohamed Khrouf ◽  
med Habib BEN Aribia ◽  
Khaled Terras ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
High Level ◽  
Abstinence Period

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Renata Simões ◽  
Weber Beringui Feitosa ◽  
Adriano Felipe Perez Siqueira ◽  
Marcilio Nichi ◽  
Fabíola Freitas Paula-Lopes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Cleavage Rate ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Significant Difference

Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

Oxidative stress adducts (OSA): impact on sperm DNA integrity

2012 ◽  
Vol 98 (3) ◽  
pp. S240
Author(s):  
R. Sethi ◽  
E. Tirado ◽  
K.-H.N. Nguyen ◽  
B. Barret ◽  
D. Sakkas
Keyword(s):  
Oxidative Stress ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna

scholarly journals Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Wiesław Bielas ◽  
Wojciech Niżański ◽  
Agnieszka Partyka ◽  
Anna Rząsa ◽  
Ryszard Mordak
Keyword(s):  
Sperm Parameters ◽  
Dna Integrity ◽  
Long Term Storage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Term Storage
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