Isolation and identification of C-19 fatty acids with anti-tumor activity from the spores of Ganoderma lucidum (reishi mushroom)

Fitoterapia ◽  
2012 ◽  
Vol 83 (3) ◽  
pp. 490-499 ◽  
Author(s):  
Pei Gao ◽  
Tomoya Hirano ◽  
Zhiqing Chen ◽  
Tadashi Yasuhara ◽  
Yoshihiro Nakata ◽  
...  
2008 ◽  
Vol 31 (10) ◽  
pp. 1933-1937 ◽  
Author(s):  
Masataka Fukuzawa ◽  
Rie Yamaguchi ◽  
Izumi Hide ◽  
Zhiqing Chen ◽  
Yuko Hirai ◽  
...  

1978 ◽  
Vol 41 (1) ◽  
pp. 28-30 ◽  
Author(s):  
ANTHONY J. DeLUCCA ◽  
STEPHEN J. PLATING ◽  
ROBERT L. ORY

Rice bran and whole brown rice are currently underutilized because free fatty acids are formed from rice oil during storage. Rough rice from two growing areas was tested for presence of lipolytic microorganisms that could release these fatty acids. Approximately 10% of the total bacterial plate count (4 × 106/g for Louisiana and 12 × 106/g for the Arkansas rice samples) were lipolytic. Upon testing, most were classified as nonsaccharolytic, alkali-producing pseudomonads. The average mold plate count for the Louisiana sample was 2 × 103 colony forming units (cfu)/g and 5 × 103 cfu/g for the Arkansas sample. All molds isolated showed various amounts of lipolytic action, as determined by the size of the lipolytic zone. The molds were generally isolated more from the Louisiana than from the Arkansas rice. Alternaria and Helminthosporium species, the most prevalent molds, were found in all samples.


1971 ◽  
Vol 49 (13) ◽  
pp. 2302-2309 ◽  
Author(s):  
S. U. Khan ◽  
M. Schnitzer

The fractionation scheme described in this and in an earlier paper resulted in the isolation and identification of about 2.0 g of pure components from 100 g of fulvic acid. About 28% of these compounds were phenolic acids, 19% benzenecarboxylic acids, 13% alkanes and fatty acids, and 40% dialkyl phthalates. The isolation of appreciable amounts of dialkyl phthalates was somewhat surprising but their presence was shown not be an artifact. The KMnO4 oxidation of the same fractions increased the yields of methylated phenolic and benzenecarboxylic acids by between 3 and 11 times.Following KMnO4 oxidation of methylated but unfractioned fulvic acid, the yield of phenolic and benzenecarboxylic acids increased substantially. The increased yields, after oxidation, of the same compounds as those that were separated without oxidation indicate that either these compounds originated from more complex structures or that they came from a structure of the type proposed earlier, which consists of phenolic and benzenecarboxylic acids joined by hydrogen-bonds, on which alkanes, fatty acids, and dialkyl phthalates are adsorbed.


2020 ◽  
Vol 10 (11) ◽  
pp. 3907 ◽  
Author(s):  
Maria Michela Salvatore ◽  
Alessandro Elvetico ◽  
Monica Gallo ◽  
Francesco Salvatore ◽  
Marina DellaGreca ◽  
...  

Ganoderma lucidum is a rich and promising source of bioactive compounds capable of positively influencing the human health. For this reason, G. lucidum is the target of vigorous investigational activities, focusing on the extraction of substances with potential biomedical applications, as well as on the improvement of analytical methodologies for their screening. In the present study, three different extraction processes (i.e., extraction with n-hexane, in Soxhlet apparatus with n-hexane and reflux with chloroform) were employed to evaluate the lipid content of G. lucidum spores. The experimental data from the produced extracts were compared with ones from the commercial liquid extract obtained with supercritical CO2 extraction of G. lucidum spores. To achieve this goal, the gas chromatography-mass spectrometry (GC-MS) methodology was optimized for an accurate and reliable identification and quantification of fatty acids in triglycerides of G. lucidum spores. Significant amounts of oleic and palmitic acids were found in the examined extracts. In particular, our findings show that economical and convenient extraction procedures, which are simple, low cost and no need for expensive tools, allow to produce extracts with a lipid composition similar to the one obtained with hard strategies of extraction.


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