scholarly journals Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum

Gene ◽  
2005 ◽  
Vol 363 ◽  
pp. 202-210 ◽  
Author(s):  
Blanca de las Rivas ◽  
Áng ela Marcobal ◽  
Alicia Gómez ◽  
Rosario Muñoz
Keyword(s):  
Lactobacillus Plantarum ◽  
Insertion Sequence

scholarly journals Promoter/repressor system of Lactobacillus plantarum phage øg1e: characterization of the promoters pR49–pR–pL and overproduction of the Cro-like protein Cng in Escherichia coli

Gene ◽  
1998 ◽  
Vol 215 (2) ◽  
pp. 371-379 ◽  
Author(s):  
Makiko Kakikawa ◽  
Nobukatsu Watanabe ◽  
Tatsuya Funawatashi ◽  
Masaya Oki ◽  
Hiroo Yasukawa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum

Insertion Sequence Based Molecular Characterization of Burkholderia mallei NCTC 3709 Strain

2014 ◽  
Vol 38 (2) ◽  
pp. 99-102
Author(s):  
Chandan Prakash ◽  
P. Das ◽  
B. V. Sunil Kumar ◽  
Bincy Joseph ◽  
Vidya Singh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Insertion Sequence ◽  
Burkholderia Mallei

scholarly journals Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family

2006 ◽  
Vol 394 (3) ◽  
pp. 575-579 ◽  
Author(s):  
Sergey V. Novoselov ◽  
Deame Hua ◽  
Alexey V. Lobanov ◽  
Vadim N. Gladyshev
Keyword(s):  
Mammalian Cells ◽  
Insertion Sequence ◽  
Phylogenetic Analyses ◽  
Dependent Manner ◽  
Putative Active Site ◽  
A Genome ◽  
Sequence Elements ◽  
Identification And Characterization ◽  
Insertion Sequence Elements

Sec (selenocysteine) is a rare amino acid in proteins. It is co-translationally inserted into proteins at UGA codons with the help of SECIS (Sec insertion sequence) elements. A full set of selenoproteins within a genome, known as the selenoproteome, is highly variable in different organisms. However, most of the known eukaryotic selenoproteins are represented in the mammalian selenoproteome. In addition, many of these selenoproteins have cysteine orthologues. Here, we describe a new selenoprotein, designated Fep15, which is distantly related to members of the 15 kDa selenoprotein (Sep15) family. Fep15 is absent in mammals, can be detected only in fish and is present in these organisms only in the selenoprotein form. In contrast with other members of the Sep15 family, which contain a putative active site composed of Sec and cysteine, Fep15 has only Sec. When transiently expressed in mammalian cells, Fep15 incorporated Sec in an SECIS- and SBP2 (SECIS-binding protein 2)-dependent manner and was targeted to the endoplasmic reticulum by its N-terminal signal peptide. Phylogenetic analyses of Sep15 family members suggest that Fep15 evolved by gene duplication.

scholarly journals Genetic and biochemical characterization of an oligo-α-1,6-glucosidase from Lactobacillus plantarum

2017 ◽  
Vol 246 ◽  
pp. 32-39 ◽  
Author(s):  
Susana Delgado ◽  
Ana Belén Flórez ◽  
Lucía Guadamuro ◽  
Baltasar Mayo
Keyword(s):  
Lactobacillus Plantarum ◽  
Biochemical Characterization

scholarly journals Characterization of Two Virulent Phages of Lactobacillus plantarum

2012 ◽  
Vol 78 (24) ◽  
pp. 8719-8734 ◽  
Author(s):  
Mariángeles Briggiler Marcó ◽  
Josiane E. Garneau ◽  
Denise Tremblay ◽  
Andrea Quiberoni ◽  
Sylvain Moineau
Keyword(s):  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
Open Reading Frames ◽  
High Identity ◽  
Content Type ◽  
Defense Systems ◽  
Double Stranded Dna ◽  
Type Phage ◽  
Reading Frames

ABSTRACTWe characterized twoLactobacillus plantarumvirulent siphophages, ATCC 8014-B1 (B1) and ATCC 8014-B2 (B2), previously isolated from corn silage and anaerobic sewage sludge, respectively. Phage B2 infected two of the eightL. plantarumstrains tested, while phage B1 infected three. Phage adsorption was highly variable depending on the strain used. Phage defense systems were found in at least twoL. plantarumstrains, LMG9211 and WCSF1. The linear double-stranded DNA genome of thepac-type phage B1 had 38,002 bp, a G+C content of 47.6%, and 60 open reading frames (ORFs). Surprisingly, the phage B1 genome has 97% identity with that ofPediococcus damnosusphage clP1 and 77% identity with that ofL. plantarumphage JL-1; these phages were isolated from sewage and cucumber fermentation, respectively. The double-stranded DNA (dsDNA) genome of thecos-type phage B2 had 80,618 bp, a G+C content of 36.9%, and 127 ORFs with similarities to those ofBacillusandLactobacillusstrains as well as phages. Some phage B2 genes were similar to ORFs fromL. plantarumphage LP65 of theMyoviridaefamily. Additionally, 6 tRNAs were found in the phage B2 genome. Protein analysis revealed 13 (phage B1) and 9 (phage B2) structural proteins. To our knowledge, this is the first report describing such high identity between phage genomes infecting different genera of lactic acid bacteria.

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