The structural and functional characterization of Malus domestica double bond reductase MdDBR provides insights towards the identification of its substrates

2021 ◽  
Vol 171 ◽  
pp. 89-99
Author(s):  
Rosanna Caliandro ◽  
Ivan Polsinelli ◽  
Nicola Demitri ◽  
Francesco Musiani ◽  
Stefan Martens ◽  
...  
Keyword(s):  
Double Bond ◽  
Malus Domestica ◽  
Functional Characterization ◽  
Double Bond Reductase

scholarly journals Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum

ACS Catalysis ◽  
2013 ◽  
Vol 3 (3) ◽  
pp. 370-379 ◽  
Author(s):  
David J. Mansell ◽  
Helen S. Toogood ◽  
John Waller ◽  
John M. X. Hughes ◽  
Colin W. Levy ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Double Bond ◽  
Nicotiana Tabacum ◽  
Double Bond Reductase

scholarly journals Characterization of 12-Oxophytodienoic Acid Reductases from Rose-scented Geranium (Pelargonium graveolens)

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101
Author(s):  
Miu Iijima ◽  
Hiromichi Kenmoku ◽  
Hironobu Takahashi ◽  
Jung-Bum Lee ◽  
Masao Toyota ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Metabolic Engineering ◽  
Double Bond ◽  
Carbonyl Compound ◽  
Catalytic Properties ◽  
Biotechnological Applications ◽  
Pelargonium Graveolens ◽  
Double Bond Reductase ◽  
Herbal Plant

Pelargonium graveolens L'Hér, also referred to as rose geranium, is a popular herbal plant with typical rosy fragrance largely based on the blend of monoterpenoid constituents. Among them, citronellol, which is biosynthesized from geraniol via double bond reduction, is the most abundant scent compound. In this study, three 12-oxophytodienoic acid reductases (PgOPR1–3) have been cloned from P. graveolens, as possible candidates for the double-bond reductase involved in citronellol biosynthesis. The bacterially expressed recombinant PgOPRs did not reduce geraniol to citronellol, but stereoselectively converted citral into ( S)-citronellal in the presence of NADPH. Thus, the α,β-unsaturated carbonyl moiety in the substrate is essential for the catalytic activity of PgOPRs, as reported for OPRs from other plants and structurally related yeast old yellow enzymes. PgOPRs promiscuously accepted linear and cyclic α,β-unsaturated carbonyl substrates, including methacrolein, a typical reactive carbonyl compound. The possible biotechnological applications for PgOPRs in plant metabolic engineering, based on their catalytic properties, are discussed herein.

An unusual desaturase in Aquilegia vulgaris

2000 ◽  
Vol 28 (6) ◽  
pp. 641-643
Author(s):  
A. J. Longman ◽  
L. V. Michaelson ◽  
O. Sayanova ◽  
J. A. Napier ◽  
A. K. Stobart
Keyword(s):  
Fatty Acid ◽  
Double Bond ◽  
Seed Oil ◽  
Membrane Lipids ◽  
Functional Characterization ◽  
Trans Configuration ◽  
Putative Gene ◽  
Specific Fatty Acid ◽  
Front End

Aquilegia vulgaris seed oil contains high levels of the rare fatty acid columbinic acid (18:3 Δ5,9,12), which is unusual in having the double bond at the Δ5 carbon in the trans configuration. Columbinic acid was found to be a seed-specific fatty acid not only present in the storage oil but also in membrane lipids. Several putative gene fragments have been isolated from plant RNA with sequences similar to previously characterized ‘front-end’ desaturases. Functional characterization of the Aquilegia cDNA is underway.

Molecular cloning and functional characterization of the CEP RECEPTOR 1 gene MdCEPR1 of Apple (Malus × domestica)

2019 ◽  
Vol 140 (3) ◽  
pp. 539-550
Author(s):  
Rui Li ◽  
Jian-Ping An ◽  
Chun-Xiang You ◽  
Xiao-Fei Wang ◽  
Yu-Jin Hao
Keyword(s):  
Molecular Cloning ◽  
Malus Domestica ◽  
Functional Characterization

scholarly journals Identification and functional characterization of the MdHB-1 gene promoter sequence from Malus×domestica

2017 ◽  
Vol 16 (8) ◽  
pp. 1730-1741 ◽  
Author(s):  
Hao-jie WANG ◽  
Yong-hua JIANG ◽  
Ying-wei QI ◽  
Jie-yu DAI ◽  
Yan-li LIU ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Functional Characterization ◽  
Gene Promoter ◽  
Promoter Sequence
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