Investigation of ellagic acid rich-berry extracts directed silver nanoparticles synthesis and their antimicrobial properties with potential mechanisms towards Enterococcus faecalis and Candida albicans

2021 ◽  
Vol 341 ◽  
pp. 155-162
Author(s):  
Semiha Ekrikaya ◽  
Ebubekir Yilmaz ◽  
Cagla Celik ◽  
Sezer Demirbuga ◽  
Nilay Ildiz ◽  
...  
2012 ◽  
Vol 13 (4) ◽  
pp. 436-439 ◽  
Author(s):  
Hamid Jafarzadeh ◽  
Maryam Bidar ◽  
Sepideh Hooshiar ◽  
Mahboubeh Naderinasab ◽  
Mostafa Moazzami ◽  
...  

ABSTRACT Aim To compare the antimicrobial effect of 2% chlorhexidine, 2.5% sodium hypochlorite and MUMS containing 2% chlorhexidine. Materials and methods All of the above irrigants were examined on Enterococcus faecalis, Streptococcus mutans, Candida albicans, Lactobacillus casei and E.coli. A total of 0.5 CC of each solution and 0.5 CC of McFarland solution bacterium were added to each examination tube. After 15, 30 and 45 minutes, colony count was performed for each tube. The difference in the number of bacteria indicated the effect taken by disinfectant material. Results MUMS containing chlorhexidine showed the antimicrobial properties just like chlorhexidine's effect against E.coli, Streptococcus mutans, Candida albicans, Enterococcus faecalis and Lactobacillus casei in preventing these entire microorganisms to incubate. Sodium hypochlorite was not effective against Enterococcus faecalis and Candida albicans incubated in 15, 30 and 45 minutes and Enterococcus faecalis in 15 minutes. Conclusion MUMS has antimicrobial properties similar to chlorhexidine. Clinical significance As MUMS containing chlorhexidine can transfer chlorhexidine through its own surfactant around apical area and it can open the dentinal tubules by its own chelator for more penetration of chlorhexidine, it may be a choice for canal irrigation. How to cite this article Bidar M, Hooshiar S, Naderinasab M, Moazzami M, Orafaee H, Naghavi N, Jafarzadeh H. Comparative Study of the Antimicrobial Effect of Three Irrigant Solutions (Chlorhexidine, Sodium Hypochlorite and Chlorhexidinated MUMS). J Contemp Dent Pract 2012;13(4): 436-439.


Author(s):  
Farah Demeri ◽  
Krishna Prasad D ◽  
VEENA SHETTY ◽  
Shriya Shetty

Aims: To evaluate the effect of silver nanoparticle, itraconazole and a combination of the two agents on the adherence of Candia albicans, Streptococcus mutans and Enterococcus faecalis on acrylic and silicone tissue conditioners. Materials and Methods: 40 pellets of Group 1 ( GC Soft Liner) and 40 of Group 2 (Sofreliner) were fabricated. The pellets were immersed in a solution of silver nanoparticles or itraconazole or a 1:1 ratio of the combination of the two medicaments. The amount of biofilm formed was assessed by the ELISA reader. Results: A decrease in adherence of Candida albicans was seen in acrylic and silicone-based tissue conditioners when compared to the untreated control samples. Silicone based tissue conditioner showed the least adherence of Candida albicans when treated with 1:1 ratio of silver nanoparticles and itraconazole. There was a statistically significant difference in microbial adhesion between acrylic and silicone tissue conditioner (p<0.001).Pellets treated with silver nanoparticles were studied for adherence of Streptococcus mutans , Candida albicans and Enterococcus faecalis. A statistically significant decrease in all three microbial adherences was observed with this group. (p<0.001) Conclusion: Silver nanoparticles and itraconazole are effective antifungal and antibacterial agents that can potentially decrease microbial colonization of Candia albicans , Streptococcus mutans and Enterococcus faecalis on tissue conditioners. Clinical implications: Silver nanoparticles and itraconazole when used will decrease biofilm formation that will not only improve the overall health of the oral cavity but also prevent denture stomatitis and diseases associated with poor oral hygiene protocols. Keywords: Silver nanoparticles, tissue conditioners, itraconazole, antimicrobial.


2020 ◽  
Vol 20 (9) ◽  
pp. 713-719 ◽  
Author(s):  
Insa Seck ◽  
Samba F. Ndoye ◽  
Lalla A. Ba ◽  
Alioune Fall ◽  
Abdoulaye Diop ◽  
...  

Background: Due to the rapid development of microbial resistance, finding new molecules became urgent to counteract this problem. Objective: The objective of this work is to access 1,2,3-triazene-1,3-disubstituted, a class of molecule with high therapeutic potential. Methods: Here we describe the access to 17 new triazene including six with an imidazole-1,2,3-triazene moiety and eleven with an alkyl-1,2,3-triazene moiety and their evaluation against five strains: two gram (-): Escherichia coli ATCC 25921 and Pseudomonas aeruginosa ATCC 27253; two gram (+) : Staphylococcus aureus ATCC 38213 and Enterococcus faecalis ATCC 29212; and one fungi: Candida albicans ATCC 24433. Results: All strains were sensitive and the best MIC, 0.28 µM, is observed for 4c against Escherichia coli ATCC 25921. Compound 9, 3-isopropynyltriazene, appears to be the most interesting since it is active on the five evaluated strains with satisfactory MIC 0.32 µM against Escherichia coli and Pseudomonas aeruginosa and 0.64 µM against Enterococcus faecalis and Pseudomonas aeruginosa. Conclusion: Comparing the structure activity relationship, electron withdrawing groups appear to increase antimicrobial activity.


2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.


Author(s):  
Marco Felipe Salas‐Orozco ◽  
Nereyda Niño‐Martínez ◽  
Gabriel‐Alejandro Martínez‐Castañón ◽  
Fernando Torres Méndez ◽  
Gabriela Margarita Montero Morán ◽  
...  

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