Analysis of creatinine in mouse and rat serum by ion exchange high performance liquid chromatography for in vivo studies of renal function

2007 ◽  
Vol 846 (1-2) ◽  
pp. 245-251 ◽  
Author(s):  
Kenneth J. Fountain ◽  
Alla Kloss ◽  
Ilya Garibyan ◽  
Bella Blitshteyn ◽  
Alexander Brezzani ◽  
...  
Keyword(s):  
Renal Function ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
High Performance ◽  
In Vivo Studies ◽  
Rat Serum

Qualitative and Quantitative Analysis of Resveratrol and Oxyresveratrol by Liquid Chromatography

2020 ◽  
Vol 7 (1) ◽  
pp. 24-31
Author(s):  
Rajeshree Khambadkar ◽  
Selvan Ravindran ◽  
Digamber Singh Chahar ◽  
Srushti Utekar ◽  
Amlesh Tambe
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pharmacokinetic Profile ◽  
Quantitative Information ◽  
In Vivo Studies ◽  
Chromatography Method ◽  
Qualitative And Quantitative

Introduction: Resveratrol and its monooxygenated metabolite oxyresveratrol were the subject matter of intense research due to their medicinal value. Absorption, distribution, metabolism and excretion are important to understand the bioavailability and pharmacokinetic profile of resveratrol and oxyresveratrol. Quantification of resveratrol and oxyresveratrol is essential for both in vitro and in vivo studies. Methods: During in vitro drug metabolism studies, both qualitative and quantitative information are essential to understand the metabolic profile of resveratrol and oxyresveratrol. In the present study, a simple and stable method is outlined using high performance liquid chromatography to quantify both resveratrol and oxyresveratrol. This method is suitable to understand the metabolic stability, plasma stability, pharmacokinetics and toxicokinetics of resveratrol and oxyresveratrol. Results: Generally, in vitro incubation studies are performed at high concentrations and in vivo studies are carried out at both high and low concentrations, therefore high performance liquid chromatography method is demonstrated as a suitable technique to quantify resveratrol and oxyresveratrol. Conclusion: Retention time of resveratrol and oxyresveratrol from liquid chromatography qualitatively confirm its identity.

Identification of the toxic components in Semen Strychni and their metabolites in rat serum by high performance liquid chromatography coupled with a Q Exactive high-resolution benchtop quadrupole Orbitrap mass spectrometer

RSC Advances ◽  
2015 ◽  
Vol 5 (95) ◽  
pp. 77689-77698 ◽  
Author(s):  
Shujuan Li ◽  
Meiyu Zhang ◽  
Pengyi Hou ◽  
Ruowen Zhang ◽  
Chenzhi Hou ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
Rat Serum ◽  
Orbitrap Mass Spectrometer ◽  
Q Exactive ◽  
Semen Strychni

Scheme of the identification of components in vitro and in vivo.

scholarly journals Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis

2019 ◽  
Vol 39 (12) ◽  
pp. 954-960
Author(s):  
Heidy C. Dos Santos ◽  
Dielson S. Vieira ◽  
Sandra M. Yamamoto ◽  
Mateus M. Costa ◽  
Maria C.A. Sá ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Minimum Bactericidal Concentration ◽  
In Vivo Studies ◽  
Propolis Extract ◽  
Adherence Test ◽  
Staphylococcus Spp

ABSTRACT: The indiscriminate use of antibiotics in the treatment of caprine mastitis causes the appearance of resistant microorganisms, besides leaving residues in milk, putting at risk to human health. In this way, propolis is an alternative in the treatment of diseases because it has antimicrobial activity, mainly because of the presence of flavonoids in its composition. The aim of this study was to evaluate the antimicrobial potential of propolis to Staphylococcus spp. Isolated from cases of goat mastitis and qualify the crude ethanoic extract by high performance liquid chromatography (HPLC). In this study, the minimum bactericidal concentration values of propolis extracts in ethanol, ethyl acetate and hexane showed that the best concentrations capable of promoting the highest mortality of the isolates of Staphylococcus spp. from mastitis in goats, were 6250, 3125 and 1562.5μg/mL, respectively. By the microplate adherence test, it was found that 20.78% isolates were not able to form biofilm, 14.70% were classified as moderate and 64.70% were weak and none as a strong biofilm producer. Propolis in its different diluents was able to affect the formation of biofilm and showed a pronounced marked antimicrobial activity against Staphylococcus spp. strains and may be indicated for use in in vivo studies.

Effects of low dietary aflatoxin B1 on broiler liver concentration without and with Mycosorb® toxin binder

2013 ◽  
Vol 2 ◽  
Author(s):  
C.A. Moran ◽  
J. Apajalahti ◽  
A. Yiannikouris ◽  
S. Ojanperä ◽  
H. Kettunen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Detection Limit ◽  
Pilot Study ◽  
High Performance ◽  
Low Doses ◽  
Dietary Concentration ◽  
Liver Concentration ◽  
The Eu

SummaryA pilot study was conducted to evaluate the suitability of hepatic aflatoxin (AFB1) concentration as a biomarker to assess the in vivo efficacy of a mycotoxin binder in poultry when AFB1 dietary concentrations are low. Diets containing low doses of AFB1 without or with Mycosorb® (MTB), a mycotoxin binder, were fed to broilers from 7 to 21 days of age. The accumulation of AFB1 in liver was measured by high performance liquid chromatography with a detection limit of 5 ng/kg liver. In response to 10 and 50 µg AFB1/kg feed, hepatic AFB1 accumulation was 27 and 145 ng AFB1/kg liver, respectively. At each dietary concentration of AFB1, the inclusion of 5 g MTB/kg of feed reduced (P < 0.1 for 10 μg AFB1/kg feed and P < 0.05 for 50 μg AFB1/kg feed) hepatic AFB1 accumulation by at least 50%. These results suggest that hepatic AFB1 concentration is a suitable biomarker for evaluating mycotoxin binder efficacy in poultry fed the EU maximum dietary concentration of 10 µg of AFB1/kg feed.

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