Application of liquid chromatography–mass spectrometry to measure short chain fatty acids in blood

2009 ◽  
Vol 877 (8-9) ◽  
pp. 719-724 ◽  
Author(s):  
Hans M.H. van Eijk ◽  
Johanne G. Bloemen ◽  
Cornelis H.C. Dejong
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Chain Fatty Acids

scholarly journals Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

2018 ◽  
Vol 56 (2) ◽  
pp. 190-197 ◽  
Author(s):  
Zhen Chen ◽  
Yue Wu ◽  
Rojeet Shrestha ◽  
Zijun Gao ◽  
Yaoyao Zhao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Chain Fatty Acids

Background Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. Methods Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. Results The developed method exhibited good linearity (R2 = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%−6.1%, with the average recoveries of 87.8%−102.4% and 92.2%−98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. Conclusion This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.

scholarly journals Glioma induced alterations in fecal short-chain fatty acids and neurotransmitters

CNS Oncology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. CNS57 ◽  
Author(s):  
Antonio Dono ◽  
Anthony Patrizz ◽  
Ryan M McCormack ◽  
Nagireddy Putluri ◽  
Bhanu P Ganesh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Healthy Controls ◽  
Liquid Chromatography Mass Spectrometry ◽  
Rrna Sequencing ◽  
Mouse Glioma ◽  
Chain Fatty Acids

Aim: To explore fecal short-chain fatty acids and neurotransmitter alterations in a mouse–glioma model and glioma patients. Methods: Liquid chromatography–mass spectrometry and 16S rRNA-sequencing from fecal samples were performed to measure metabolite levels and taxa abundance in mice/humans. Mice underwent GL261 implantation with/without temozolomide. Glioma patients were compared with healthy controls. Results: Glioma altered several short-chain fatty acids and neurotransmitter levels. Reduced 5-hydroxyindoleaceic acid and norepinephrine levels were seen in mice and humans. Interestingly, temozolomide treatment abrogates the effects of glioma on fecal metabolites. Conclusion: Our findings demonstrate the interplay between glioma and the gut–brain axis. Further work is required to identify pathways within the gut–brain axis by which glioma influences and promotes the modulation of fecal metabolites and microbiome.

Method for Absolute Quantification of Short Chain Fatty Acids via Reverse Phase Chromatography Mass Spectrometry

2019 ◽  
Author(s):  
Dominique Bihan ◽  
- Thomas Rydzak ◽  
Madeleine Wyss ◽  
Keir Pittman ◽  
Kathy D. McCoy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Absolute Quantification ◽  
Short Chain ◽  
Reverse Phase ◽  
Stable Isotope Dilution ◽  
Chromatography Mass Spectrometry ◽  
Chain Fatty Acids

<p>Short chain fatty acids (SCFAs; including acetate, propionate, and butyrate) are an important class of biological molecules that play a major role in modulating host-microbiome interactions. Despite significant research into SCFA-mediated biological mechanisms, absolute quantification of these molecules by liquid chromatography mass spectrometry (LC-MS) is challenging due to their relatively poor chromatographic properties and low mass. Herein, we introduce SQUAD, a quantitative strategy for analyzing SCFAs using an aniline-based derivatization approach in conjunction with reverse-phase LC-MS/MS analysis. We show that this approach, when coupled to quantification by stable isotope dilution (SID), enables absolute quantification of biologically relevant SCFAs in complex biological samples. We evaluate the limits of detection using this approach, analyze sources of error affecting quantification, and provide practical guidelines for using this strategy. Moreover, we illustrate the utility of this technique via two representative biological applications where SCFA analysis is common: 1) SCFA quantification in the caecal contents of germ free versus conventionally raised specific pathogen free mice and 2) in an analysis of <i>in vitro </i>microbial cultures. </p>

scholarly journals A Facile Profiling Method of Short Chain Fatty Acids Using Liquid Chromatography-Mass Spectrometry

Metabolites ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 173 ◽  
Author(s):  
Ha Eun Song ◽  
Hyo Yeong Lee ◽  
Su Jung Kim ◽  
Sung Hoon Back ◽  
Hyun Ju Yoo
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Human Body ◽  
Biological Samples ◽  
Short Chain Fatty Acids ◽  
The Body ◽  
Short Chain ◽  
Simple Method ◽  
Chain Fatty Acids

Short chain fatty acids (SCFAs) are the main products of dietary fibers that are not digested by the human body, and they have been shown to affect human metabolism and inflammation. The amount of SCFAs in the body is related to many human diseases, and studies have focused on elucidating their roles and target molecules in both metabolic and immune responses. Thus, the quantitation of SCFAs in biological samples becomes crucial in understanding their important roles in the human body. Herein, a facile profiling method of SCFAs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and then applied to biological samples. C2-C6 SCFAs were derivatized while using 4-acetamido-7-mercapto-2,1,3-benzoxadiazole for 5 min. at room temperature prior to LC-MS/MS analysis, and characteristic fragmentation patterns and increased hydrophobicity after chemical derivatization enabled specific discrimination among 12 SCFAs. Derivatization was fast and reliable, and the reaction products were stable for a week at 4 °C. The developed method was applied to measure SCFAs in mouse feces, plasma, and human exhaled breath condensates. This fast and simple method can save labor and effort to profile SCFAs from various biological samples.

Method for Absolute Quantification of Short Chain Fatty Acids via Reverse Phase Chromatography Mass Spectrometry

2019 ◽  
Author(s):  
Dominique Bihan ◽  
- Thomas Rydzak ◽  
Madeleine Wyss ◽  
Keir Pittman ◽  
Kathy D. McCoy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Absolute Quantification ◽  
Short Chain ◽  
Reverse Phase ◽  
Stable Isotope Dilution ◽  
Chromatography Mass Spectrometry ◽  
Chain Fatty Acids

<p>Short chain fatty acids (SCFAs; including acetate, propionate, and butyrate) are an important class of biological molecules that play a major role in modulating host-microbiome interactions. Despite significant research into SCFA-mediated biological mechanisms, absolute quantification of these molecules by liquid chromatography mass spectrometry (LC-MS) is challenging due to their relatively poor chromatographic properties and low mass. Herein, we introduce SQUAD, a quantitative strategy for analyzing SCFAs using an aniline-based derivatization approach in conjunction with reverse-phase LC-MS/MS analysis. We show that this approach, when coupled to quantification by stable isotope dilution (SID), enables absolute quantification of biologically relevant SCFAs in complex biological samples. We evaluate the limits of detection using this approach, analyze sources of error affecting quantification, and provide practical guidelines for using this strategy. Moreover, we illustrate the utility of this technique via two representative biological applications where SCFA analysis is common: 1) SCFA quantification in the caecal contents of germ free versus conventionally raised specific pathogen free mice and 2) in an analysis of <i>in vitro </i>microbial cultures. </p>

Sign in / Sign up

Export Citation Format

Share Document