Comparison of Glycated Hemoglobin Results Based on At-Home and In-Lab Dried Blood Spot Sampling to Routine Venous Blood Sampling In-Lab in Adult Patients With Type 1 or Type 2 Diabetes

2018 ◽  
Vol 42 (4) ◽  
pp. 426-432.e1 ◽  
Author(s):  
Thomas G. Elliott ◽  
Kent C. Dooley ◽  
Mira Zhang ◽  
Harlan S.D. Campbell ◽  
Darby J.S. Thompson
Keyword(s):  
Type 2 Diabetes ◽  
Glycated Hemoglobin ◽  
Venous Blood ◽  
Adult Patients ◽  
Blood Sampling ◽  
Dried Blood Spot ◽  
Venous Blood Sampling ◽  
Blood Spot

scholarly journals Dried Blood Spot Analysis Suitable for Therapeutic Drug Monitoring of Voriconazole, Fluconazole, and Posaconazole

2013 ◽  
Vol 57 (10) ◽  
pp. 4999-5004 ◽  
Author(s):  
Kim C. M. van der Elst ◽  
Lambert F. R. Span ◽  
Kai van Hateren ◽  
Karin M. Vermeulen ◽  
Tjip S. van der Werf ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Sampling Method ◽  
Fungal Infections ◽  
Venous Blood ◽  
Blood Sampling ◽  
Dried Blood Spot ◽  
Therapeutic Drug ◽  
Venous Blood Sampling ◽  
Blood Spot

ABSTRACTInvasive aspergillosis and candidemia are important causes of morbidity and mortality in immunocompromised and critically ill patients. The triazoles voriconazole, fluconazole, and posaconazole are widely used for the treatment and prophylaxis of these fungal infections. Due to the variability of the pharmacokinetics of the triazoles among and within individual patients, therapeutic drug monitoring is important for optimizing the efficacy and safety of antifungal treatment. A dried blood spot (DBS) analysis was developed and was clinically validated for voriconazole, fluconazole, and posaconazole in 28 patients. Furthermore, a questionnaire was administered to evaluate the patients' opinions of the sampling method. The DBS analytical method showed linearity over the concentration range measured for all triazoles. Results for accuracy and precision were within accepted ranges; samples were stable at room temperature for at least 12 days; and different hematocrit values and blood spot volumes had no significant influence. The ratio of the drug concentration in DBS samples to that in plasma was 1.0 for voriconazole and fluconazole and 0.9 for posaconazole. Sixty percent of the patients preferred DBS analysis as a sampling method; 15% preferred venous blood sampling; and 25% had no preferred method. There was significantly less perception of pain with the DBS sampling method (P= 0.021). In conclusion, DBS analysis is a reliable alternative to venous blood sampling and can be used for therapeutic drug monitoring of voriconazole, fluconazole, and posaconazole. Patients were satisfied with DBS sampling and had less pain than with venous sampling. Most patients preferred DBS sampling to venous blood sampling.

scholarly journals Dried blood spot versus venous blood sampling for phenylalanine and tyrosine

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Kimber van Vliet ◽  
Wiggert G. van Ginkel ◽  
Esther van Dam ◽  
Pim de Blaauw ◽  
Martijn Koehorst ◽  
...  
Keyword(s):  
Venous Blood ◽  
Blood Sampling ◽  
Dried Blood Spot ◽  
Venous Blood Sampling ◽  
Blood Spot

scholarly journals Dried blood spot versus venous blood sampling for phenylalanine and tyrosine

2020 ◽  
Author(s):  
Kimber van Vliet ◽  
Wiggert G. van Ginkel ◽  
Esther van Dam ◽  
Pim de Blaauw ◽  
Martijn Koehorst ◽  
...  
Keyword(s):  
Venous Blood ◽  
Dried Blood Spot ◽  
Liquid Chromatography Mass Spectrometry ◽  
Constant Bias ◽  
Amino Acid Analyzer ◽  
Edta Plasma ◽  
Venous Blood Sampling ◽  
Blood Spot ◽  
Good Agreement

Abstract BACKGROUND: To investigate agreement between various dried blood spot (DBS) and venous blood sample measurements of phenylalanine and tyrosine concentrations in Phenylketonuria (PKU) and Tyrosinemia type 1 (TT1) patients.STUDY DESIGN: Phenylalanine and tyrosine concentrations were studied in 45 PKU/TT1 patients in plasma from venous blood in lithium heparin (LH) and EDTA tubes; venous blood from LH and EDTA tubes on a DBS card; venous blood directly on a DBS card; and capillary blood on a DBS card. Plasma was analyzed with an amino acid analyzer and DBS were analyzed with liquid chromatography-mass spectrometry. Agreement between different methods was assessed using Passing and Bablok fit and Bland Altman analyses. RESULTS: In general, phenylalanine concentrations in LH plasma were comparable to capillary DBS, whereas tyrosine concentrations were slightly higher in LH plasma (constant bias of 6.4 µmol/L). However, in the low phenylalanine range, most samples had higher phenylalanine concentrations in DBS compared to LH plasma. Remarkably, phenylalanine and tyrosine in EDTA plasma were higher compared to all other samples (slopes ranging from 7-12%). No differences were observed when comparing capillary DBS to other DBS.CONCLUSIONS: Overall agreement between plasma and DBS is good. However, bias is specimen- (LH vs EDTA), and possibly concentration- (low phenylalanine) dependent. Because of the overall good agreement, we recommend the use of a DBS-plasma correction factor for DBS measurement. Each laboratory should determine their own factor dependent on filter card type, extraction and calibration protocols taking the LH plasma values as gold standard.

scholarly journals Dried blood spot versus venous blood sampling for phenylalanine and tyrosine: a large difference?

2019 ◽  
Author(s):  
Kimber van Vliet ◽  
Wiggert G. van Ginkel ◽  
Esther van Dam ◽  
Pim de Blaauw ◽  
Martijn Koehorst ◽  
...  
Keyword(s):  
Venous Blood ◽  
Dried Blood Spot ◽  
Liquid Chromatography Mass Spectrometry ◽  
Constant Bias ◽  
Amino Acid Analyzer ◽  
Edta Plasma ◽  
Venous Blood Sampling ◽  
Blood Spot ◽  
Good Agreement

Abstract BACKGROUND: To investigate agreement between various dried blood spot (DBS) and venous blood sample measurements of phenylalanine and tyrosine concentrations in Phenylketonuria (PKU) and Tyrosinemia type 1 (TT1) patients. STUDY DESIGN: Phenylalanine and tyrosine concentrations were studied in 45 PKU/TT1 patients in plasma from venous blood in lithium heparin (LH) and EDTA tubes; venous blood from LH and EDTA tubes on a DBS card; venous blood directly on a DBS card; and capillary blood on a DBS card. Plasma was analyzed with an amino acid analyzer and DBS were analyzed with liquid chromatography-mass spectrometry. Agreement between different methods was assessed using Passing and Bablok fit and Bland Altman analyses. RESULTS: In general, phenylalanine concentrations in LH plasma were comparable to capillary DBS, whereas tyrosine concentrations were slightly higher in LH plasma (constant bias of 6.4 µmol/L). However, in the low phenylalanine range, most samples had higher phenylalanine concentrations in DBS compared to LH plasma. Remarkably, phenylalanine and tyrosine in EDTA plasma were higher compared to all other samples (slopes ranging from 7-12%). No differences were observed when comparing capillary DBS to other DBS. CONCLUSIONS: Overall agreement between plasma and DBS is good. However, bias is specimen- (LH vs EDTA), and possibly concentration- (low phenylalanine) dependent. Because of the overall good agreement, we recommend the use of a DBS-plasma correction factor for DBS measurement. Each laboratory should determine their own factor dependent on filter card type, extraction and calibration protocols taking the LH plasma values as gold standard.

Dried blood spot sampling of nilotinib in patients with chronic myeloid leukaemia: a comparison with venous blood sampling

2017 ◽  
Vol 69 (10) ◽  
pp. 1265-1274 ◽  
Author(s):  
Christel C.L.M. Boons ◽  
Abdel Chahbouni ◽  
Anneliene M. Schimmel ◽  
Abraham J. Wilhelm ◽  
Yvonne M. den Hartog ◽  
...  
Keyword(s):  
Chronic Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Venous Blood ◽  
Blood Sampling ◽  
Dried Blood Spot ◽  
Venous Blood Sampling ◽  
Blood Spot

scholarly journals Gut microbiota imbalances in Tunisian participants with type 1 and type 2 diabetes mellitus

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Meriem Fassatoui ◽  
Mireia Lopez-Siles ◽  
Diana A. Díaz-Rizzolo ◽  
Haifa Jmel ◽  
Chokri Naouali ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Multivariate Analysis ◽  
Gut Microbiota ◽  
Glycated Hemoglobin ◽  
Statistical Tests ◽  
Treatment Of Diabetes

Abstract Gut microbiota plays an important role in the regulation of the immune system and host’s metabolism. We aimed to characterize the gut microbiota of Tunisian participants with and without diabetes. We enrolled ten participants with type 1 diabetes mellitus (T1DM), ten patients with type 2 diabetes mellitus (T2DM), and 11 subjects without diabetes. Bacteria was quantified in fecal samples by quantitative PCR (qPCR). Statistical tests and multivariate analysis were performed using RStudio program. Results showed that the proportions of Firmicutes, Akkermansia muciniphila, and Faecalibacterium prausnitzii (P≤0.041), as well as, the ratio Firmicutes/Bacteroidetes decreased in participants with T1DM compared with those without diabetes (p = 0.036). Participants with T2DM presented a reduction in the amounts of A. muciniphila and F. prausnitzii compared with those without diabetes (P≤0.036). Furthermore, A muciniphila is negatively correlated with glucose level (P=0.022) and glycated hemoglobin (HbA1c) (P=0.035). Multivariate analysis revealed that participants with diabetes formed a cluster apart compared with those without diabetes. In conclusion the gut bacteria of Tunisian participants with diabetes was altered. The gut bacterial profile, especially the distribution of A muciniphila in participants with diabetes was affected by glycemic dysregulation. The investigation of the gut microbiota may help clinicians to improve diagnosis and treatment of diabetes and its complications.

scholarly journals Relationship Between Glycated Hemoglobin and Metabolic Syndrome of Type 1 and Type 2 Diabetes: A factor analysis study

Diabetes Care ◽  
2010 ◽  
Vol 33 (6) ◽  
pp. e80-e80 ◽  
Author(s):  
F. M. A. Giuffrida ◽  
C. F. C. Sallum ◽  
M. A. L. Gabbay ◽  
M. B. Gomes ◽  
A. C. Pires ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Metabolic Syndrome ◽  
Factor Analysis ◽  
Glycated Hemoglobin ◽  
Analysis Study

scholarly journals Glycated Albumin is a Better Indicator for Glucose Excursion than Glycated Hemoglobin in Type 1 and Type 2 Diabetes

2008 ◽  
Vol 55 (3) ◽  
pp. 503-507 ◽  
Author(s):  
Kazutomi YOSHIUCHI ◽  
Munehide MATSUHISA ◽  
Naoto KATAKAMI ◽  
Yoshihisa NAKATANI ◽  
Kenya SAKAMOTO ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Glycated Hemoglobin ◽  
Glycated Albumin ◽  
Glucose Excursion
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