scholarly journals Effects of Panax notoginseng saponins on proliferation and differentiation of rat embryonic cortical neural stem cells

2016 ◽  
Vol 79 (5) ◽  
pp. 256-263 ◽  
Author(s):  
Yinchu Si ◽  
Jun Zhu ◽  
Xiang Huang ◽  
Peichun Zhu ◽  
Chune Xie
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Panax Notoginseng ◽  
Panax Notoginseng Saponins ◽  
Proliferation And Differentiation

Effects of Panax Notoginseng Saponins on Proliferation and Differentiation of Rat Hippocampal Neural Stem Cells

2011 ◽  
Vol 39 (05) ◽  
pp. 999-1013 ◽  
Author(s):  
Yin-Chu Si ◽  
Jian-Ping Zhang ◽  
Chun-E Xie ◽  
Li-Juan Zhang ◽  
Xiang-Ning Jiang
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Ischemia Reperfusion ◽  
Panax Notoginseng ◽  
Control Group ◽  
Panax Notoginseng Saponins ◽  
Double Labeling ◽  
Proliferation And Differentiation ◽  
Hippocampal Neural Stem Cells

We aimed to investigate the effects of Panax notoginseng saponins (PNS) on proliferation, differentiation and self-renewal of rat hippocampal neural stem cells (NSCs) in vitro. Rat hippocampal NSCs were isolated from post-natal day 1 (P1) rats and cultured in a serum-free medium. The neurospheres were identified by the expressions of nestin, class III β-tublin (Tuj-1) and glial fibrillary acid protein (GFAP). The cells were given PNS and subjected to oxygen glucose deprivation (OGD) as an in vitro model of brain ischemia reperfusion. The proliferation of NSCs was determined by MTT colorimetry, nestin/BrdU immunofluorescent double-labeling and RT-PCR. Differentiation of NSCs was assessed by immunofluorescent double-labeling of nestin/BrdU, nestin/vimentin, and nestin/Tuj-1. The primary cells and the first two passages of cells formed certain amount of neurospheres, the cells derived from a single cell clone also formed neurospheres. Nestin, BrdU, GFAP and Tuj-1-positive cells appeared in those neurospheres. Compared to the control group, PNS significantly promoted NSC proliferation and the expression of nestin/BrdU, and also enhanced Tuj-1, vimentin, and nestin mRNA expressions in hippocampal NSCs. PNS significantly increased area density, optical density and numbers of nestin/BrdU, nestin/vimentin, and nestin/Tuj-1 positive cells following OGD. These results indicate that PNS can promote proliferation and differentiation of hippocampus NCSs in vitro after OGD, suggesting its potential benefits on neurogenesis and neuroregeneration in brain ischemic injury.

Astragalus Flavone Induces Proliferation and Differentiation of Neural Stem Cells in a Cerebral Infarction Model

2021 ◽  
Vol 28 ◽  
Author(s):  
Han Gao ◽  
Ningjing Huang ◽  
Weiwei Wang ◽  
Lingling Zhang ◽  
Li Cai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerebral Infarction ◽  
Neural Stem Cells ◽  
Proliferation And Differentiation

Schisandrin A and B Affect the Proliferation and Differentiation of Neural Stem Cells

2021 ◽  
pp. 102058
Author(s):  
Ning-Ning Cai ◽  
Qi Geng ◽  
Yu Jiang ◽  
Wen-Qian Zhu ◽  
Rui Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Proliferation And Differentiation

scholarly journals Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Heliyon ◽  
2017 ◽  
Vol 3 (6) ◽  
pp. e00318 ◽  
Author(s):  
Melanie Bender ◽  
Lisa Schwind ◽  
David Grundmann ◽  
Monika Martin ◽  
Markus Klotz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase ◽  
Neural Stem Cells ◽  
Protein Kinase Ck2 ◽  
Proliferation And Differentiation ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

2014 ◽  
Vol 38 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Shuang‐Qing Chen ◽  
Qing Cai ◽  
Yu‐Ying Shen ◽  
Xiu‐Ying Cai ◽  
Hai‐Ying Lei
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Combined Use ◽  
Proliferation And Differentiation

scholarly journals Neuroprotective effects of dexmedetomidine on the ketamine-induced disruption of the proliferation and differentiation of developing neural stem cells in the subventricular zone

2020 ◽  
Author(s):  
Huanhuan Sha ◽  
Peipei Peng ◽  
Bing Li ◽  
Guohua Wei ◽  
Juan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Subventricular Zone ◽  
Pediatric Anesthesia ◽  
Brdu Incorporation ◽  
Control Group ◽  
Neuroprotective Effects ◽  
Proliferation And Differentiation ◽  
Ketamine Anesthesia ◽  
Β Tubulin

Abstract Background: Recently, the number of neonatal patients receiving surgery under general anesthesia has increased. Ketamine disrupts the proliferation and differentiation of developing neural stem cells (NSCs). Therefore, the safe use of ketamine in pediatric anesthesia has been an issue of increasing concern among anesthesiologists and the children’s parents. Dexmedetomidine (DEX) is widely used in sedation, as an antianxiety agent and for analgesia. DEX has recently been shown to provide neuroprotection against anesthetic-induced neurotoxicity in the developing brain. The aim of this in vivo study was to investigate whether DEX exerted neuroprotective effects on the proliferation and differentiation of NSCs in the subventricular zone (SVZ) following neonatal ketamine exposure. Methods: Postnatal day 7 (PND-7) male Sprague-Dawley rats were equally divided into the following 5 groups: Control group (n=8), Ketamine group (n=8), 1 μg/kg DEX+Ketamine group (n=8), 5 μg/kg DEX+Ketamine group (n=8) and 10 μg/kg DEX+Ketamine group (n=8). The proliferation and differentiation of NSCs in the SVZ were assessed using immunostaining with BrdU incorporation. The levels of Nestin and β-tubulin III in the SVZ were measured using Western blot analyses. Apoptosis was assessed by detecting the levels of the cleaved caspase-3 protein using Western blotting. Results: Neonatal ketamine exposure significantly inhibited NSC proliferation and astrocytic differentiation in the SVZ, and neuronal differentiation was markedly increased. Furthermore, pretreatment with moderate (5 μg/kg) or high doses (10 μg/kg) of DEX reversed the ketamine-induced disturbances in the proliferation and differentiation of NSCs. Meanwhile, neonatal ketamine exposure significantly decreased the expression of Nestin and increased the expression of β-tubulin III in the SVZ compared with the Control group. Treatment with 10 μg/kg DEX notably reversed the ketamine-induced changes in the levels of Nestin and β-tubulin III. In addition, a pretreatment with 10 μg/kg DEX before ketamine anesthesia prevented apoptosis in the SVZ induced by neonatal ketamine exposure. Conclusions: Based on our findings, DEX may exert neuroprotective effects on the proliferation and differentiation of NSCs in the SVZ of neonatal rats in a repeated ketamine anesthesia model.

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