Evaluation of pH-responsive liposomes containing amino acid-based zwitterionic lipids for improving intracellular drug delivery in vitro and in vivo

2010 ◽  
Vol 142 (2) ◽  
pp. 267-276 ◽  
Author(s):  
Yosuke Obata ◽  
Shoji Tajima ◽  
Shinji Takeoka
Keyword(s):  
Drug Delivery ◽  
Amino Acid ◽  
Ph Responsive ◽  
Intracellular Drug Delivery ◽  
Zwitterionic Lipids

scholarly journals Single Domain Antibodies as Carriers for Intracellular Drug Delivery: A Proof of Principle Study

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 927
Author(s):  
Sebas D. Pronk ◽  
Erik Schooten ◽  
Jurgen Heinen ◽  
Esra Helfrich ◽  
Sabrina Oliveira ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Single Domain ◽  
Drug Conjugates ◽  
Intracellular Drug Delivery ◽  
Internalization Rate ◽  
Single Domain Antibodies ◽  
Different Characteristics

Antibody-drug conjugates (ADCs) are currently used for the targeted delivery of drugs to diseased cells, but intracellular drug delivery and therefore efficacy may be suboptimal because of the large size, slow internalization and ineffective intracellular trafficking of the antibody. Using a phage display method selecting internalizing phages only, we developed internalizing single domain antibodies (sdAbs) with high binding affinity to rat PDGFRβ, a receptor involved in different types of diseases. We demonstrate that these constructs have different characteristics with respect to internalization rates but all traffic to lysosomes. To compare their efficacy in targeted drug delivery, we conjugated the sdAbs to a cytotoxic drug. The conjugates showed improved cytotoxicity correlating to their internalization speed. The efficacy of the conjugates was inhibited in the presence of vacuolin-1, an inhibitor of lysosomal maturation, suggesting lysosomal trafficking is needed for efficient drug release. In conclusion, sdAb constructs with different internalization rates can be designed against the same target, and sdAbs with a high internalization rate induce more cell killing than sdAbs with a lower internalization rate in vitro. Even though the overall efficacy should also be tested in vivo, sdAbs are particularly interesting formats to be explored to obtain different internalization rates.

Improved in vivo tumor therapy via host–guest complexation

2016 ◽  
Vol 4 (15) ◽  
pp. 2691-2696 ◽  
Author(s):  
Yong Yao ◽  
Yang Wang ◽  
Ruibo Zhao ◽  
Li Shao ◽  
Ruikang Tang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Intracellular Ph ◽  
Tumor Therapy ◽  
Controlled Drug Delivery ◽  
Water Soluble ◽  
Ph Responsive ◽  
Mesoporous Nanoparticles

A decomposable and intracellular pH-responsive drug delivery system by immobilizing a water-soluble pillar[5]arene onto hollow mesoporous nanoparticles through host–guest complexation was successfully prepared and its application in controlled drug delivery in vitro and in vivo was also investigated.

Fabrication of a pH responsive DOX conjugated PEGylated palladium nanoparticle mediated drug delivery system: an in vitro and in vivo evaluation

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 44998-45014 ◽  
Author(s):  
Krishnamurthy Shanthi ◽  
Karuppaiya Vimala ◽  
Dhanaraj Gopi ◽  
Soundarapandian Kannan
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Hela Cells ◽  
Ph Responsive ◽  
Palladium Nanoparticle ◽  
In Vivo Evaluation ◽  
Induced Apoptosis

Schematic illustration of the possible mechanism of pH based drug delivery system of DOX conjugated PEGylated PdNPs induced apoptosis in HeLa cells.

Anti-tumor drug delivery system based on cyclodextrin-containing pH-responsive star polymer: In vitro and in vivo evaluation

2014 ◽  
Vol 474 (1-2) ◽  
pp. 232-240 ◽  
Author(s):  
Qingqing Xiong ◽  
Mingming Zhang ◽  
Zhibao Zhang ◽  
Wei Shen ◽  
Lingrong Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Star Polymer ◽  
Ph Responsive ◽  
In Vivo Evaluation ◽  
Tumor Drug Delivery
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