Restoration of Internalin-B Increases the Virulence of the Epidemic F2365 Listeria monocytogenes Strain

2018 ◽  
Vol 158 ◽  
pp. 133
Author(s):  
I.M. Rodríguez-Gómez ◽  
J.J. Quereda ◽  
P. Titos-Jiménez ◽  
L. Carrasco ◽  
M.A. Nahori ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Internalin B

Development of a Surface Plasmon Resonance–Based Immunoassay for Listeria monocytogenes

2005 ◽  
Vol 68 (4) ◽  
pp. 728-735 ◽  
Author(s):  
PAUL LEONARD ◽  
STEPHEN HEARTY ◽  
GARY WYATT ◽  
JOHN QUINN ◽  
RICHARD O'KENNEDY
Keyword(s):  
Surface Plasmon Resonance ◽  
Listeria Monocytogenes ◽  
Surface Plasmon ◽  
Polyclonal Antibody ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Chip Surface ◽  
Metal Affinity ◽  
Coefficients Of Variation ◽  
Internalin B

A polyclonal antibody was produced against Internalin B (InlB)–enriched extract and used to develop an inhibition assay to detect Listeria monocytogenes cells in solution using surface plasmon resonance. The gene sequence encoding for the InlB protein was cloned into a Qiagen pQE-60 vector, expressed in Escherichia coli, and purified by immobilized metal affinity chromatography. Protein G–purified anti-InlB–enriched extract polyclonal antibody was incubated with various concentrations of L. monocytogenes cells and subsequently injected over a purified-recombinant InlB (rInlB)–immobilized CM5 sensor chip surface. A decrease in antibody binding response was observed with increasing L. monocytogenes cell concentrations. Intraday and interday assay variability studies were carried out to evaluate precision and reproducibility. The assay had a limit of detection of less than 2 × 105 cells per ml and could be successfully reproduced with coefficients of variation of between 2.5 and 7.7%.

scholarly journals Reassessing the role of internalin B in Listeria monocytogenes virulence using the epidemic strain F2365

2019 ◽  
Vol 25 (2) ◽  
pp. 252.e1-252.e4 ◽  
Author(s):  
J.J. Quereda ◽  
I.M. Rodríguez-Gómez ◽  
J. Meza-Torres ◽  
J. Gómez-Laguna ◽  
M.A. Nahori ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Epidemic Strain ◽  
Internalin B

Folding and Stability of the Leucine-rich Repeat Domain of Internalin B from Listeria monocytogenes

2004 ◽  
Vol 337 (2) ◽  
pp. 453-461 ◽  
Author(s):  
Alexander Freiberg ◽  
Matthias P. Machner ◽  
Wolfgang Pfeil ◽  
Wolf-Dieter Schubert ◽  
Dirk W. Heinz ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Leucine Rich Repeat ◽  
Leucine Rich Repeat Domain ◽  
Repeat Domain ◽  
Internalin B

Virulence potential of Listeria monocytogenes strains recovered from pigs in Spain

2020 ◽  
Vol 187 (11) ◽  
pp. e101-e101
Author(s):  
Jaime Gómez-Laguna ◽  
Fernando Cardoso-Toset ◽  
Jazmín Meza-Torres ◽  
Javier Pizarro-Cerdá ◽  
Juan J Quereda
Keyword(s):  
Listeria Monocytogenes ◽  
Virulence Factors ◽  
Bacterial Pathogen ◽  
Listeriolysin O ◽  
Actin Assembly ◽  
Virulence Potential ◽  
Serotype 1 ◽  
Internalin B ◽  
Meat Samples ◽  
Internalin A

BackgroundListeria monocytogenes is a foodborne bacterial pathogen that causes listeriosis, an infectious disease in animals and people, with pigs acting as asymptomatic reservoirs. In August 2019 an outbreak associated with the consumption of pork meat caused 222 human cases of listeriosis in Spain. Determining the diversity as well as the virulence potential of strains from pigs is important to public health.MethodsThe behaviour of 23 L monocytogenes strains recovered from pig tonsils, meat and skin was compared by studying (1) internalin A, internalin B, listeriolysin O, actin assembly-inducing protein and PrfA expression levels, and (2) their invasion and intracellular growth in eukaryotic cells.ResultsMarked differences were found in the expression of the selected virulence factors and the invasion and intracellular replication phenotypes of L monocytogenes strains. Strains obtained from meat samples and belonging to serotype 1/2a did not have internalin A anchored to the peptidoglycan. Some strains expressed higher levels of the studied virulence factors and invaded and replicated intracellularly more efficiently than an epidemic L monocytogenes reference strain (F2365).ConclusionThis study demonstrates the presence of virulent L monocytogenes strains with virulent potential in pigs, with valuable implications in veterinary medicine and food safety.

scholarly journals A tandem repeat of a fragment of Listeria monocytogenes internalin B protein induces cell survival and proliferation

2010 ◽  
Vol 299 (6) ◽  
pp. L905-L914 ◽  
Author(s):  
Ognoon Mungunsukh ◽  
Young H. Lee ◽  
Ana P. Marquez ◽  
Fabiola Cecchi ◽  
Donald P. Bottaro ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Disulfide Bonds ◽  
Large Scale ◽  
Ex Vivo ◽  
Complex Structure ◽  
Potential Candidate ◽  
Scale Production ◽  
Amino Terminal ◽  
Linker Peptide ◽  
Internalin B

Hepatocyte growth factor (HGF) is critical for tissue homeostasis and repair in many organs including the lung, heart, kidney, liver, nervous system, and skin. HGF is a heterodimeric protein containing 20 disulfide bonds distributed among an amino-terminal hairpin, four kringle domains, and a serine protease-like domain. Due to its complex structure, recombinant production of HGF in prokaryotes requires denaturation and refolding, processes that are impractical for large-scale manufacture. Thus, pharmaceutical quantities of HGF are not available despite its potential applications. A fragment of the Listeria monocytogenes internalin B protein from amino acids 36–321 (InlB36–321) was demonstrated to bind to and partially activate the HGF receptor Met. InlB36–321 has a stable β-sheet structure and is easily produced in its native conformation by Escherichia coli . We cloned InlB36–321 (1×InlB36–321) and engineered a head-to-tail repeat of InlB36–321 with a linker peptide (2×InlB36–321); 1×InlB36–321 and 2×InlB36–321 were purified from E. coli . Both 1× and 2×InlB36–321 activated the Met tyrosine kinase. We subsequently compared signal transduction of the two proteins in primary lung endothelial cells. 2×InlB36–321 activated ERK1/2, STAT3, and phosphatidylinositol 3-kinase/Akt pathways, whereas 1×InlB36–321 activated only STAT3 and ERK1/2. The 2×InlB36–321 promoted improved motility compared with 1×InlB36–321 and additionally stimulated proliferation equivalent to full-length HGF. Both the 1× and 2×InlB36–321 prevented apoptosis by the profibrotic peptide angiotensin II in cell culture and ex vivo lung slice cultures. The ease of large-scale production and capacity of 2×InlB36–321 to mimic HGF make it a potential candidate as a pharmaceutical agent for tissue repair.

scholarly journals Listeria monocytogenes Internalin B Activates Junctional Endocytosis to Accelerate Intestinal Invasion

2010 ◽  
Vol 6 (5) ◽  
pp. e1000900 ◽  
Author(s):  
Mickey Pentecost ◽  
Jyothi Kumaran ◽  
Partho Ghosh ◽  
Manuel R. Amieva
Keyword(s):  
Listeria Monocytogenes ◽  
Internalin B

scholarly journals Lactiplantibacillus plantarum subsp. plantarum and Fructooligosaccharides Combination Inhibits the Growth, Adhesion, Invasion, and Virulence of Listeria monocytogenes

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 170
Author(s):  
Qingli Dong ◽  
Xinxin Lu ◽  
Binru Gao ◽  
Yangtai Liu ◽  
Muhammad Zohaib Aslam ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Incubation Temperature ◽  
Foodborne Pathogen ◽  
Untreated Group ◽  
Listeriolysin O ◽  
Regulatory Factor ◽  
Gene Expressions ◽  
Internalin B ◽  
Serotype 4B ◽  
Internalin A

Listeria monocytogenes is a foodborne pathogen responsible for many food outbreaks worldwide. This study aimed to investigate the single and combined effect of fructooligosaccharides (FOS) and Lactiplantibacillus plantarum subsp. plantarum CICC 6257 (L. plantarum) on the growth, adhesion, invasion, and virulence of gene expressions of Listeria monocytogenes 19112 serotype 4b (L. monocytogenes). Results showed that L. plantarum combined with 2% and 4% (w/v) FOS significantly (p < 0.05) inhibited the growth of L. monocytogenes (3–3.5 log10 CFU/mL reduction) at the incubation temperature of 10 °C and 25 °C. Under the same combination condition, the invasion rates of L. monocytogenes to Caco-2 and BeWo cells were reduced more than 90% compared to the result of the untreated group. After L. plantarum was combined with the 2% and 4% (w/v) FOS treatment, the gene expression of actin-based motility, sigma factor, internalin A, internalin B, positive regulatory factor A, and listeriolysin O significantly (p < 0.05) were reduced over 91%, 77%, 92%, 89%, 79%, and 79% compared to the result of the untreated group, respectively. The inhibition level of the L. plantarum and FOS combination against L. monocytogenes was higher than that of FOS or L. plantarum alone. Overall, these results indicated that the L. plantarum and FOS combination might be an effective formula against L. monocytogenes.

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