Exercise is an important stressor and is correlated with cytokine production in several tissues. There is little information about changes in interleukin (IL)-6 and IL-1b in four-beat gaited horses during a typical aerobic exercise challenge. The objective of this research was to characterise changes in plasma IL-6, IL-1b, cortisol and other blood biomarkers in four-beat gaited horses after a marcha simulation test. We subjected 12 fit horses (approximately 5 years old and 390 kg) to the marcha simulation test (MST) (i.e. a 10 min warm-up, 30 min at marcha and 15 min cool down). Blood samples were collected before the MST, immediately after the MST and 15 and 120 min after the MST (i.e. recovery). Results were analysed with One Way Repeated Measures ANOVA and a Tukey tests with P≤0.05 and Pearson correlation test. The highest value of plasma IL-6 was observed immediately after MST (6.85 pg/ml) (P≥0.05). IL-1 and cortisol did not change (P≥0.05). However, creatine kinase (CK) and aspartate aminotransferase, nonesterified fatty acid (NEFA), glycerol, total cholesterol and glucose were elevated immediately after the MST and at 15 min after the MST (P≤0.05). No change was observed in alanine aminotransferase and triglycerides (P>0.05). Medium correlation was observed between NEFA and glycerol (R=0.64) and glycerol and triglycerides (R=0.50). In conclusion, an increase in IL-6 immediately that occurred after marcha simulation test was associated with elevated concentrations of several energetic metabolites (NEFA, glycerol and glucose). This metabolic adaption may contributed to the horses’ performance during their typical aerobic exercise and was not associated with significant elevation in IL-1b, CK, and cortisol in healthy trained four-beat gaited horses.
Marcha Gait Simulation Test Decrease Antioxidative Biomarkers in Four-Beat Gaited Horses