Viscoelastic characterization of rat cerebral cortex and type I collagen scaffolds for central nervous system tissue engineering

2012 ◽  
Vol 12 ◽  
pp. 63-73 ◽  
Author(s):  
Paul Z. Elias ◽  
Myron Spector
Keyword(s):  
Tissue Engineering ◽  
Central Nervous System ◽  
Nervous System ◽  
Cerebral Cortex ◽  
Type I Collagen ◽  
Rat Cerebral Cortex ◽  
Type I ◽  
Central Nervous System Tissue ◽  
Collagen Scaffolds

scholarly journals Materials for Central Nervous System Tissue Engineering

10.5772/59339 ◽  
2014 ◽  
Author(s):  
Manuel Pérez-Garnes ◽  
Juan A. Barcia ◽  
Ulises Gómez-Pinedo ◽  
Manuel Monleón Pradas ◽  
Ana Vallés-Lluch
Keyword(s):  
Tissue Engineering ◽  
Central Nervous System ◽  
Nervous System ◽  
Central Nervous System Tissue

Comparison and characterization of type I collagen extracted using different methods for applications in tissue engineering

2008 ◽  
Vol 136 ◽  
pp. S411-S412
Author(s):  
Xin Xiong ◽  
Robin Ghosh ◽  
Ekkehard Hiller ◽  
Friedel Drepper ◽  
Herwig Brunner ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Type I

scholarly journals Differential Spatiotemporal Expression of Type I and Type II Cadherins Associated With the Segmentation of the Central Nervous System and Formation of Brain Nuclei in the Developing Mouse

2021 ◽  
Vol 14 ◽  
Author(s):  
Julie Polanco ◽  
Fredy Reyes-Vigil ◽  
Sarah D. Weisberg ◽  
Ilirian Dhimitruka ◽  
Juan L. Brusés
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cerebral Cortex ◽  
Cell Adhesion ◽  
Basal Ganglia ◽  
Mrna Expression ◽  
Expression Pattern ◽  
Type I ◽  
Type Ii ◽  
Brain Nuclei

Type I and type II classical cadherins comprise a family of cell adhesion molecules that regulate cell sorting and tissue separation by forming specific homo and heterophilic bonds. Factors that affect cadherin-mediated cell-cell adhesion include cadherin binding affinity and expression level. This study examines the expression pattern of type I cadherins (Cdh1, Cdh2, Cdh3, and Cdh4), type II cadherins (Cdh6, Cdh7, Cdh8, Cdh9, Cdh10, Cdh11, Cdh12, Cdh18, Cdh20, and Cdh24), and the atypical cadherin 13 (Cdh13) during distinct morphogenetic events in the developing mouse central nervous system from embryonic day 11.5 to postnatal day 56. Cadherin mRNA expression levels obtained from in situ hybridization experiments carried out at the Allen Institute for Brain Science (https://alleninstitute.org/) were retrieved from the Allen Developing Mouse Brain Atlas. Cdh2 is the most abundantly expressed type I cadherin throughout development, while Cdh1, Cdh3, and Cdh4 are expressed at low levels. Type II cadherins show a dynamic pattern of expression that varies between neuroanatomical structures and developmental ages. Atypical Cdh13 expression pattern correlates with Cdh2 in abundancy and localization. Analyses of cadherin-mediated relative adhesion estimated from their expression level and binding affinity show substantial differences in adhesive properties between regions of the neural tube associated with the segmentation along the anterior–posterior axis. Differences in relative adhesion were also observed between brain nuclei in the developing subpallium (basal ganglia), suggesting that differential cell adhesion contributes to the segregation of neuronal pools. In the adult cerebral cortex, type II cadherins Cdh6, Cdh8, Cdh10, and Cdh12 are abundant in intermediate layers, while Cdh11 shows a gradated expression from the deeper layer 6 to the superficial layer 1, and Cdh9, Cdh18, and Cdh24 are more abundant in the deeper layers. Person’s correlation analyses of cadherins mRNA expression patterns between areas and layers of the cerebral cortex and the nuclei of the subpallium show significant correlations between certain cortical areas and the basal ganglia. The study shows that differential cadherin expression and cadherin-mediated adhesion are associated with a wide range of morphogenetic events in the developing central nervous system including the organization of neurons into layers, the segregation of neurons into nuclei, and the formation of neuronal circuits.

Molecular characterization of Type I GABAA receptor complex from rat cerebral cortex and hippocampus

1994 ◽  
Vol 25 (3-4) ◽  
pp. 225-233 ◽  
Author(s):  
Diego Ruano ◽  
Francisco Araujo ◽  
Alberto Machado ◽  
Angel L. de Blas ◽  
Javier Vitorica
Keyword(s):  
Cerebral Cortex ◽  
Molecular Characterization ◽  
Gabaa Receptor ◽  
Rat Cerebral Cortex ◽  
Type I ◽  
Receptor Complex ◽  
Gabaa Receptor Complex

Abnormalities in Central Nervous System Development in Osteogenesis Imperfecta Type II

1999 ◽  
Vol 2 (2) ◽  
pp. 124-130 ◽  
Author(s):  
Shawn Clark Emery ◽  
Nancy C. Karpinski ◽  
Lawrence Hansen ◽  
Eliezer Masliah
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
White Matter ◽  
Osteogenesis Imperfecta ◽  
Type I Collagen ◽  
Nervous System Development ◽  
Type I ◽  
Osteogenesis Imperfecta Type ◽  
Type Ii ◽  
Neuroblast Migration

Osteogenesis imperfecta (OI) type II is a perinatally lethal condition resulting from mutations in type I collagen genes. In addition to characteristic skeletal anomalies, OI type II has recently been shown to be associated with neuropathological alterations, specifically perivenous microcalcifications, and impaired neuroblast migration. In light of these findings, and because type I collagen promotes neuritic maturation both in vitro and in vivo, we sought to determine if additional central nervous system (CNS) developmental anomalies could be found in previously autopsied OI type II cases, and if specific abnormalities correlate with OI subtypes. We retrospectively studied brains of nine patients diagnosed with OI. Of these, seven were OI type II: five were OI type IIA, one was type IIB, and one was type IIC. One OI type I specimen and one OI type III brain were included for comparison, as well as five controls. The IIC brain showed hippocampal malrotation, agyria, abnormal neuronal lamination, diffuse hemorrhage, and peri-ventricular leukomalacia (PVL). The IIB brain had white matter gliosis, PVL, and perivascular calcifications, but was normally developed. Of the five type IIA brains, two showed migrational defects with coexisting PVL and gliosis, two were normally developed with similar white matter injuries, and one was grossly normal. These findings support the contention that collagen mutations might negatively impact CNS development.

scholarly journals Aligned and Conductive 3D Collagen Scaffolds for Skeletal Muscle Tissue Engineering

2020 ◽  
Author(s):  
Ivan M. Basurto ◽  
Mark T. Mora ◽  
Gregg M. Gardner ◽  
George J. Christ ◽  
Steven R. Caliari
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Type I Collagen ◽  
Structural Alignment ◽  
Skeletal Muscle Tissue ◽  
Type I ◽  
Muscle Injuries ◽  
Collagen Scaffolds ◽  
3D Collagen

AbstractSkeletal muscle is characterized by its three-dimensional (3D) anisotropic architecture composed of highly aligned, organized, and electrically excitable muscle fibers that enable normal locomotion. Biomaterial-based tissue engineering approaches to repair skeletal muscle injuries are limited due to difficulties in combining 3D structural alignment (to guide cell/matrix organization) and electrical conductivity (to enable electrically excitable myotube assembly and maturation). In this work we successfully produced aligned and electrically conductive 3D collagen scaffolds using a freeze-drying approach. Conductive polypyrrole (PPy) microparticles were synthesized and directly mixed into a suspension of type I collagen and chondroitin sulfate followed by directional lyophilization. Scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and confocal microscopy analyses showed that directional solidification resulted in scaffolds with longitudinally aligned macropores (transverse plane: 155 ± 27 µm, longitudinal plane: 218 ± 49 µm) with homogeneously-distributed PPy content. Chronopotentiometry verified that PPy incorporation resulted in a five-fold increase in conductivity when compared to non-PPy containing collagen scaffolds without detrimentally affecting C2C12 mouse myoblast metabolic activity. Furthermore, the aligned scaffold microstructure provided contact guidance cues that directed myoblast growth and organization. Incorporation of PPy also promoted enhanced myotube formation and maturation as measured by myosin heavy chain (MHC) expression and number of nuclei per myotube. Together these data suggest that aligned and conductive 3D collagen scaffolds could be useful for skeletal muscle tissue engineering.

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