Myogenic differentiation 1 and transcription factor 12 activate the gene expression of mouse taste receptor type 1 member 1

2021 ◽  
Author(s):  
Yui Obikane ◽  
Takashi Toyono ◽  
Shoichiro Kokabu ◽  
Kae Matsuyama ◽  
Shinji Kataoka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Myogenic Differentiation ◽  
Taste Receptor ◽  
Receptor Type ◽  
Mouse Taste

scholarly journals Multiple sweet receptors and transduction pathways revealed in knockout mice by temperature dependence and gurmarin sensitivity

2009 ◽  
Vol 296 (4) ◽  
pp. R960-R971 ◽  
Author(s):  
Tadahiro Ohkuri ◽  
Keiko Yasumatsu ◽  
Nao Horio ◽  
Masafumi Jyotaki ◽  
Robert F. Margolskee ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Knockout Mice ◽  
Taste Receptor ◽  
Sweet Taste ◽  
Receptor Type ◽  
Temperature Sensitive ◽  
Chorda Tympani ◽  
Temperature Dependent ◽  
Mouse Taste

Sweet taste transduction involves taste receptor type 1, member 2 (T1R2), taste receptor type 1, member 3 (T1R3), gustducin, and TRPM5. Because knockout (KO) mice lacking T1R3, gustducin's Gα subunit (Gαgust), or TRPM5 exhibited greatly reduced, but not abolished responses of the chorda tympani (CT) nerve to sweet compounds, it is likely that multiple sweet transduction pathways exist. That gurmarin (Gur), a sweet taste inhibitor, inhibits some but not all mouse CT responses to sweet compounds supports the existence of multiple sweet pathways. Here, we investigated Gur inhibition of CT responses to sweet compounds as a function of temperature in KO mice lacking T1R3, Gαgust, or TRPM5. In T1R3-KO mice, responses to sucrose and glucose were Gur sensitive (GS) and displayed a temperature-dependent increase (TDI). In Gαgust-KO mice, responses to sucrose and glucose were Gur-insensitive (GI) and showed a TDI. In TRPM5-KO mice, responses to glucose were GS and showed a TDI. All three KO mice exhibited no detectable responses to SC45647, and their responses to saccharin displayed neither GS nor a TDI. For all three KO mice, the lingual application of pronase, another sweet response inhibitor, almost fully abolished responses to sucrose and glucose but did not affect responses to saccharin. These results provide evidence for 1) the existence of multiple transduction pathways underlying responses to sugars: a T1R3-independent GS pathway for sucrose and glucose, and a TRPM5-independent temperature sensitive GS pathway for glucose; 2) the requirement for Gαgust in GS sweet taste responses; and 3) the existence of a sweet independent pathway for saccharin, in mouse taste cells on the anterior tongue.

scholarly journals Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 437
Author(s):  
Ting Gong ◽  
Weiyong Wang ◽  
Houqiang Xu ◽  
Yi Yang ◽  
Xiang Chen ◽  
...  
Keyword(s):  
Sexual Maturity ◽  
Cell Function ◽  
Expression Patterns ◽  
Taste Receptor ◽  
Receptor Type ◽  
Mrna Levels ◽  
Early Puberty ◽  
Specific Expression

Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15–180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). During development, testicular T1R3 exhibited stage-dependent and cell-specific expression patterns. In particular, T1R3 levels increased significantly from prepuberty to puberty (p < 0.05), and expression remained high until sexual maturity (p < 0.05), similar to results for phospholipase Cβ2 (PLCβ2). The strong expressions of T1R3/PLCβ2 were observed at the cytoplasm of elongating/elongated spermatids and Leydig cells. In the eight-stage cycle of the seminiferous epithelium in pigs, T1R3/PLCβ2 levels were higher in the spermatogenic epithelium at stages II–VI than at the other stages, and the strong expressions were detected in elongating/elongated spermatids and residual bodies. The message RNA (mRNA) levels of taste receptor type 1 subunit 1 (T1R1) in the testis showed a similar trend to levels of T1R3. These data indicate a possible role of T1R3 in the regulation of spermatid differentiation and Leydig cell function.

scholarly journals Orphan nuclear receptor oestrogen-related receptor γ (ERRγ) plays a key role in hepatic cannabinoid receptor type 1-mediated induction of CYP7A1 gene expression

2015 ◽  
Vol 470 (2) ◽  
pp. 181-193 ◽  
Author(s):  
Yaochen Zhang ◽  
Don-Kyu Kim ◽  
Ji-Min Lee ◽  
Seung Bum Park ◽  
Won-IL Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bile Acid ◽  
Nuclear Receptor ◽  
Cannabinoid Receptor ◽  
Inverse Agonist ◽  
Receptor Type ◽  
Orphan Nuclear Receptor ◽  
Transcription Regulator ◽  
Estrogen Related Receptor

ERRγ is a novel transcription regulator of CYP7A1 (cholesterol 7α-hydroxylase). An ERRγ (Estrogen-related receptor γ) inverse agonist modulates bile acid homoeostasis via regulation of CYP7A1 gene expression.

scholarly journals Psychosocial Stress and Cannabinoid Drugs Affect Acetylation of α-tubulin (K40) and Gene Expression in the Prefrontal Cortex of Adult Mice

2021 ◽  
Author(s):  
Jordi Tomas-Roig ◽  
Shyam Sundar Ramasamy ◽  
Diana Zbarsky ◽  
Ursula Havemann-Reinecke ◽  
Sigrid Hoyer-Fender
Keyword(s):  
Gene Expression ◽  
Prefrontal Cortex ◽  
Psychosocial Stress ◽  
Cannabinoid Receptor ◽  
Brain Plasticity ◽  
Receptor Type ◽  
Tubulin Acetylation ◽  
Cannabinoid Receptor Type 1 ◽  
The Impact

Abstract The dynamics of neuronal microtubules are essential for brain plasticity. Vesicular transport and synaptic transmission, additionally, requires acetylation of α-tubulin, and aberrant tubulin acetylation and neurobiological deficits are associated. Prolonged exposure to a stressor or consumption of drugs of abuse, like marihuana, lead to neurological changes and psychotic disorders. Here, we studied the effect of psychosocial stress and the administration of cannabinoid receptor type 1 drugs on α-tubulin acetylation in different brain regions of mice. We found significantly decreased tubulin acetylation in the prefrontal cortex and the dorsal striatum in stressed mice. The impact of cannabinoid drugs on stress-induced microtubule disturbance was investigated by administration of the cannabinoid receptor agonist WIN55,212-2 and/or antagonist rimonabant. In both, control and stressed mice, the administration of WIN55,212-2 significantly increased the tubulin acetylation in the prefrontal cortex whereas administration of both cannabinoid drugs acted antagonistically indicating a cannabinoid receptor type 1 mediated effect. The analysis of gene expression in the prefrontal cortex showed a consistent expression of ApoE attributable to either psychosocial stress or administration of the cannabinoid agonist. Additionally, ApoE expression inversely correlated with acetylated tubulin levels when comparing controls and stressed mice treated with WIN55,212-2 whereas rimonabant treatment showed the opposite.

scholarly journals On the Emerging Role of the Taste Receptor Type 1 (T1R) Family of Nutrient-Sensors in the Musculoskeletal System

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 469 ◽  
Author(s):  
Shoichiro Kokabu ◽  
Jonathan Lowery ◽  
Takashi Toyono ◽  
Tsuyoshi Sato ◽  
Tetsuya Yoda
Keyword(s):  
Musculoskeletal System ◽  
Taste Receptor ◽  
Receptor Type
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