scholarly journals Circulating platelet aggregates damage endothelial cells in culture

2017 ◽  
Vol 213 ◽  
pp. 90-99
Author(s):  
Chandrakala Aluganti Narasimhulu ◽  
Mukesh Nandave ◽  
Diana Bonilla ◽  
Janani Singaravelu ◽  
Chittoor B. Sai-Sudhakar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture ◽  
Circulating Platelet Aggregates ◽  
Platelet Aggregates

Platelets Stimulate Thromboplastin Synthesis in Human Endothelial Cells

1983 ◽  
Vol 49 (02) ◽  
pp. 069-072 ◽  
Author(s):  
U L H Johnsen ◽  
T Lyberg ◽  
K S Galdal ◽  
H Prydz
Keyword(s):  
Endothelial Cells ◽  
De Novo ◽  
Umbilical Vein ◽  
Time Dependent ◽  
De Novo Synthesis ◽  
Human Endothelial Cells ◽  
Tumor Promotor ◽  
Coagulation Assay ◽  
Platelet Aggregates ◽  
Umbilical Vein Endothelial Cells

SummaryHuman umbilical vein endothelial cells in culture synthesize thromboplastin upon stimulation with phytohaemagglutinin (PHA) or the tumor promotor 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The thromboplastin activity is further strongly enhanced in a time dependent reaction by the presence of gel-filtered platelets or platelet aggregates. This effect was demonstrable at platelet concentrations lower than those normally found in plasma, it may thus be of pathophysiological relevance. The thromboplastin activity increased with increasing number of platelets added. Cycloheximide inhibited the increase, suggesting that de novo synthesis of the protein component of thromboplastin, apoprotein III, is necessary.When care was taken to remove monocytes no thromboplastin activity and no apoprotein HI antigen could be demonstrated in suspensions of gel-filtered platelets, platelets aggregated with thrombin or homogenized platelets when studied with a coagulation assay and an antibody neutralization technique.

scholarly journals Relationship between tissue factor expression and deposition of fibrin, platelets, and leukocytes on cultured endothelial cells under venous blood flow conditions

Blood ◽  
1993 ◽  
Vol 81 (8) ◽  
pp. 2050-2058 ◽  
Author(s):  
D Kirchhofer ◽  
KS Sakariassen ◽  
M Clozel ◽  
TB Tschopp ◽  
P Hadvary ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Cross Section ◽  
Leukocyte Adhesion ◽  
Venous Blood ◽  
Cell Contact ◽  
Venous Blood Flow ◽  
Necrosis Factor Alpha ◽  
Endothelial Surface ◽  
Platelet Aggregates

Abstract Endothelial cell-mediated coagulation and leukocyte adhesion are processes that might be connected by the generation of thrombin. To examine the interaction of procoagulant and proadhesive activity, cultures of endothelial cells were stimulated with tumor necrosis factor-alpha, which resulted in the surface expression of tissue factor. Subsequent exposure to human nonanticoagulated blood at a shear rate of 100 s-1 in a parallel plate perfusion device led to the deposition of polymerized fibrin, which covered 63% of the endothelial surface. In addition, numerous platelet aggregates (71 per 10 mm cross- section) and leukocytes (53 +/- 6/mm2) were deposited on stimulated endothelial cells, whereas no fibrin and only a few platelet aggregates (4 +/- 1 per 10 mm cross-section) and leukocytes (6 +/- 1/mm2) were detected on control cells. A significant portion of the adherent leukocytes bound to fibrin and platelets. However, when the deposition of fibrin and platelet aggregates was inhibited with the anti-tissue factor antibody HTFI-7B8 by 100% and 86%, respectively, leukocyte adherence remained unchanged (68 +/- 6/mm2). This indicated that leukocytes could efficiently adhere to endothelial cells through direct cell-cell contact independent of both thrombin and deposited fibrin. Moreover, this direct adhesion of leukocytes to the endothelial surface was reduced twofold to threefold when fibrin deposition occurred. These data suggest a relationship between endothelial procoagulant and proadhesive properties in that tissue factor-initiated coagulation may contribute to leukocyte adhesion through the formation of an adhesive fibrin/platelet meshwork but concurrently prevents the adhesive endothelial surface to bind leukocytes at its full capacity.

Human Fetal Endothelial Cells in Culture

2009 ◽  
Vol 14 (4) ◽  
pp. 233-241 ◽  
Author(s):  
Tore Henriksen ◽  
Stein A. Evensen ◽  
Ragna Følling Elgjo ◽  
Anne Vefling
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture

ENDOTHELIAL CELLS IN CULTURE: A MODEL FOR STUDYING VASCULAR FUNCTIONS

2000 ◽  
Vol 42 (1) ◽  
pp. 9-19 ◽  
Author(s):  
TIZIANA BACHETTI ◽  
LUCIA MORBIDELLI
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture
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