Evaluation of a co-extraction method for real-time PCR-based body fluid identification and DNA typing

2014 ◽  
Vol 16 (1) ◽  
pp. 56-59 ◽  
Author(s):  
Ken Watanabe ◽  
Yasuki Iwashima ◽  
Tomoko Akutsu ◽  
Kazumasa Sekiguchi ◽  
Koichi Sakurada
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Extraction Method ◽  
Body Fluid ◽  
Dna Typing ◽  
Body Fluid Identification ◽  
Fluid Identification

Messenger RNA profiling: A novel method for body fluid identification by Real-Time PCR

2006 ◽  
Vol 157 (2-3) ◽  
pp. 181-186 ◽  
Author(s):  
Christa Nussbaumer ◽  
Elisabeth Gharehbaghi-Schnell ◽  
Irina Korschineck
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Messenger Rna ◽  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Rna Profiling ◽  
Novel Method

A novel application of real-time RT-LAMP for body fluid identification: using HBB detection as the model

2015 ◽  
Vol 11 (2) ◽  
pp. 208-215 ◽  
Author(s):  
Chih-Wen Su ◽  
Chiao-Yun Li ◽  
James Chun-I Lee ◽  
Dar-Der Ji ◽  
Shu-Ying Li ◽  
...  
Keyword(s):  
Real Time ◽  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification

scholarly journals Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Diagnostics ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 693
Author(s):  
Koichi Sakurada ◽  
Ken Watanabe ◽  
Tomoko Akutsu
Keyword(s):  
Body Fluid ◽  
Dna Typing ◽  
Decisive Role ◽  
Vaginal Fluid ◽  
Body Fluid Identification ◽  
Sexual Crimes ◽  
Fluid Identification ◽  
Criminal Investigations ◽  
Confirmatory Assay ◽  
Identification Of Persons

Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

scholarly journals MicroRNA markers for forensic body fluid identification obtained from miRCURY™ LNA array

2015 ◽  
Vol 5 ◽  
pp. e630-e632 ◽  
Author(s):  
X.Y. Luo ◽  
Z.L. Li ◽  
D. Peng ◽  
L. Wang ◽  
L. Zhang​ ◽  
...  
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification

mRNA profiling for body fluid identification

2008 ◽  
Vol 1 (1) ◽  
pp. 37-38 ◽  
Author(s):  
C. Haas ◽  
B. Klesser ◽  
A. Kratzer ◽  
W. Bär
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Profiling

Evaluation of the inclusion of circular RNAs in mRNA profiling in forensic body fluid identification

2017 ◽  
Vol 132 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Yaqi Zhang ◽  
Baonian Liu ◽  
Chengchen Shao ◽  
Hongmei Xu ◽  
Aimin Xue ◽  
...  
Keyword(s):  
Body Fluid ◽  
Circular Rnas ◽  
Body Fluid Identification ◽  
Fluid Identification ◽  
Mrna Profiling

scholarly journals Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains

2013 ◽  
Vol 7 (4) ◽  
pp. 418-421 ◽  
Author(s):  
Mari L. Uchimoto ◽  
Emma Beasley ◽  
Natalie Coult ◽  
Emma J. Omelia ◽  
Damian World ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Body Fluid ◽  
Pcr Analysis ◽  
Stem Loop

Optimal small-molecular reference RNA for RT-qPCR-based body fluid identification

2018 ◽  
Vol 37 ◽  
pp. 135-142 ◽  
Author(s):  
Shuntaro Fujimoto ◽  
Sho Manabe ◽  
Chie Morimoto ◽  
Munetaka Ozeki ◽  
Yuya Hamano ◽  
...  
Keyword(s):  
Body Fluid ◽  
Body Fluid Identification ◽  
Fluid Identification
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