OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17:K+:H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis

2013 ◽  
Vol 64 ◽  
pp. 1-5 ◽  
Author(s):  
James R. Johnson ◽  
Thomas A. Russo ◽  
Sarah M. Drawz ◽  
Connie Clabots ◽  
Ruth Olson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Animal Models ◽  
Tract Infection ◽  
Coli Strain ◽  
Clonal Group ◽  
Subcutaneous Infection ◽  
Systemic Sepsis ◽  
Group A

scholarly journals Iha from an Escherichia coli Urinary Tract Infection Outbreak Clonal Group A Strain Is Expressed In Vivo in the Mouse Urinary Tract and Functions as a Catecholate Siderophore Receptor

2006 ◽  
Vol 74 (6) ◽  
pp. 3427-3436 ◽  
Author(s):  
Simon Léveillé ◽  
Mélissa Caza ◽  
James R. Johnson ◽  
Connie Clabots ◽  
Mourad Sabri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Growth Promotion ◽  
Clonal Group ◽  
E Coli ◽  
Group A ◽  
K 12

ABSTRACT Virulence factors of pathogenic Escherichia coli belonging to a recently emerged and disseminated clonal group associated with urinary tract infection (UTI), provisionally designated clonal group A (CGA), have not been experimentally investigated. We used a mouse model of ascending UTI with CGA member strain UCB34 in order to identify genes of CGA that contribute to UTI. iha was identified to be expressed by strain UCB34 in the mouse kidney using selective capture of transcribed sequences. iha from strain UCB34 demonstrated a siderophore receptor phenotype when cloned in a catecholate siderophore receptor-negative E. coli K-12 strain, as shown by growth promotion experiments and uptake of 55Fe complexed to enterobactin or its linear 2, 3-dihydroxybenzoylserine (DHBS) siderophore derivatives. Siderophore-mediated growth promotion by Iha was TonB dependent. Growth and iron uptake were more marked with linear DHBS derivatives than with purified enterobactin. The reported phenotype of adherence to epithelial cells conferred by expressing iha from a multicopy cloning vector in a poorly adherent E. coli K-12 host strain was confirmed to be specific to iha, in comparison with other siderophore receptor genes. iha expression was regulated by the ferric uptake regulator Fur and by iron availability, as shown by real-time reverse transcriptase PCR. In a competitive infection experiment using the mouse UTI model, wild-type strain UCB34 significantly outcompeted an isogenic iha null mutant. Iha thus represents a Fur-regulated catecholate siderophore receptor that, uniquely, exhibits an adherence-enhancing phenotype and is the first described urovirulence factor identified in a CGA strain.

scholarly journals Detection of Clonal Group A Escherichia coli Isolates from Broiler Chickens, Broiler Chicken Meat, Community-Dwelling Humans, and Urinary Tract Infection (UTI) Patients and Their Virulence in a Mouse UTI Model

2010 ◽  
Vol 76 (24) ◽  
pp. 8281-8284 ◽  
Author(s):  
Lotte Jakobsen ◽  
Anette M. Hammerum ◽  
Niels Frimodt-Møller
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Chicken Meat ◽  
Community Dwelling ◽  
Clonal Group ◽  
Group A

ABSTRACT Escherichia coli clonal group A isolates cause infections in people. We investigated 158 phylogroup D E. coli isolates from animals, meat, and humans. Twenty-five of these isolates were of clonal group A, and 15 isolates were shown to cause infection in a mouse urinary tract infection (UTI) model. We conclude that clonal group A isolates are found in both broiler chickens and broiler chicken meat and may cause UTI in humans.

scholarly journals Recovery of a Functional Class 2 Integron from an Escherichia coli Strain Mediating a Urinary Tract Infection

2008 ◽  
Vol 52 (11) ◽  
pp. 4153-4154 ◽  
Author(s):  
Carolina Márquez ◽  
Maurizio Labbate ◽  
Ana J. Ingold ◽  
Piklu Roy Chowdhury ◽  
Maria S. Ramírez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Coli Strain ◽  
Functional Class ◽  
Signal Peptidase

ABSTRACT A class 2 integron was found in an Escherichia coli isolate mediating a urinary tract infection. Unlike other class 2 integrons from pathogens, the encoded IntI2 protein was functional. The integron possessed a dfrA14 cassette, and a second novel cassette in which a lipoprotein signal peptidase gene is predicted.

A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.

1997 ◽  
Vol 41 (9) ◽  
pp. 2041-2046 ◽  
Author(s):  
A Bauernfeind ◽  
S Wagner ◽  
R Jungwirth ◽  
I Schneider ◽  
D Meyer
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Amino Acid ◽  
Tract Infection ◽  
Coli Strain ◽  
Beta Lactamase ◽  
Beta Lactams ◽  
Beta Lactamases ◽  
Pcr Product

An Escherichia coli strain resistant to a broad spectrum of beta-lactams, including cephamycins, was isolated from a patient suffering from urinary tract infection. A resistance plasmid (pMVP-7) was transferred from the clinical isolate to an Escherichia coli recipient. Both strains produce a cefoxitin-hydrolyzing beta-lactamase focusing at pI 6.7. The phenotype was similar to that of a Klebsiella pneumoniae strain producing cephamycinase FOX-1, so primers were selected from the FOX-1 sequence to amplify the bla gene of the transconjugant. The PCR product obtained was sequenced. The percentage of identity of the deduced amino acid sequence with sequences of other AmpC-type beta-lactamases was 96.9% with FOX-1, 74.9% with CMY-1, and 67.7% with MOX-1. This new plasmid-mediated enzyme is most closely related to FOX-1 (11 amino acid exchanges). We therefore propose the designation FOX-2.

scholarly journals Translational Efficacy of Humanized Exposures of Cefepime, Ertapenem, and Levofloxacin against Extended-Spectrum-β-Lactamase-Producing Escherichia coli in a Murine Model of Complicated Urinary Tract Infection

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Marguerite L. Monogue ◽  
David P. Nicolau
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Animal Models ◽  
Tract Infection ◽  
Murine Model ◽  
Complicated Urinary Tract Infection ◽  
Novel Therapies ◽  
Content Type ◽  
Extended Spectrum

ABSTRACT Validated animal models are required as bridging tools to assess the utility of novel therapies and potential microbiologic outcomes. Herein, we utilized uropathogenic extended-spectrum-β-lactamase (ESBL)-producing and non-ESBL-producing Escherichia coli in the neutropenic murine complicated urinary tract infection (cUTI) model with humanized exposures of cefepime, ertapenem, and levofloxacin to assess its translational value to human outcomes. Our data support the translational utility of this murine model to cUTI in humans as humanized exposures produced microbiologic outcomes consistent with the phenotypic profiles of the organisms.

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