Adhesive mechanism of different Salmonella fimbrial adhesins

2019 ◽  
Vol 137 ◽  
pp. 103748 ◽  
Author(s):  
Tayyab Rehman ◽  
Lizi Yin ◽  
Muhammad Bilal Latif ◽  
Jiehao Chen ◽  
Kaiyu Wang ◽  
...  
Keyword(s):  
Fimbrial Adhesins

scholarly journals Crystallization and preliminary X-ray diffraction analysis of the Csu pili CsuC–CsuA/B chaperone–major subunit pre-assembly complex fromAcinetobacter baumannii

2015 ◽  
Vol 71 (6) ◽  
pp. 770-774 ◽  
Author(s):  
Natalia Pakharukova ◽  
Minna Tuittila ◽  
Sari Paavilainen ◽  
Anton Zavialov
Keyword(s):  
Diffusion Method ◽  
X Ray Diffraction ◽  
Hanging Drop ◽  
Unit Cell Parameters ◽  
Gram Negative ◽  
X Ray ◽  
Cell Parameters ◽  
Abiotic Surfaces ◽  
Vapour Diffusion ◽  
Fimbrial Adhesins

The attachment of many Gram-negative pathogens to biotic and abiotic surfaces is mediated by fimbrial adhesins, which are assembledviathe classical, alternative and archaic chaperone–usher (CU) pathways. The archaic CU fimbrial adhesins have the widest phylogenetic distribution, yet very little is known about their structure and mechanism of assembly. To elucidate the biogenesis of archaic CU systems, structural analysis of the Csu fimbriae, which are used byAcinetobacter baumanniito form stable biofilms and cause nosocomial infection, was focused on. The major fimbriae subunit CsuA/B complexed with the CsuC chaperone was purified from the periplasm ofEscherichia colicells co-expressing CsuA/B and CsuC, and the complex was crystallized in PEG 3350 solution using the hanging-drop vapour-diffusion method. Selenomethionine-labelled CsuC–CsuA/B complex was purified and crystallized under the same conditions. The crystals diffracted to 2.40 Å resolution and belonged to the hexagonal space groupP6422, with unit-cell parametersa=b= 94.71,c = 187.05 Å, α = β = 90, γ = 120°. Initial phases were derived from a single anomalous diffraction (SAD) experiment using the selenomethionine derivative.

Clustering of Escherichia coli Type-1 Fimbrial Adhesins by Using Multimeric Heptyl α-D-Mannoside Probes with a Carbohydrate Core

2011 ◽  
Vol 17 (36) ◽  
pp. 10029-10038 ◽  
Author(s):  
Mehdi Almant ◽  
Vincent Moreau ◽  
José Kovensky ◽  
Julie Bouckaert ◽  
Sébastien G. Gouin
Keyword(s):  
Escherichia Coli ◽  
Fimbrial Adhesins

scholarly journals Microfluidic PCR Combined with Pyrosequencing for Identification of Allelic Variants with Phenotypic Associations among Targeted Salmonella Genes

2012 ◽  
Vol 78 (20) ◽  
pp. 7480-7482 ◽  
Author(s):  
Min Yue ◽  
Robert Schmieder ◽  
Robert A. Edwards ◽  
Shelley C. Rankin ◽  
Dieter M. Schifferli
Keyword(s):  
Genetic Variation ◽  
Antimicrobial Resistance ◽  
Host Species ◽  
Massive Parallel Sequencing ◽  
Allelic Variants ◽  
Parallel Sequencing ◽  
Content Type ◽  
Potential Applications ◽  
Fimbrial Adhesins ◽  
Diagnostic Microbiology

ABSTRACTA novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46Salmonellastrains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.

scholarly journals Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli

Biology ◽  
2013 ◽  
Vol 2 (3) ◽  
pp. 894-917 ◽  
Author(s):  
Emanuela Lonardi ◽  
Kristof Moonens ◽  
Lieven Buts ◽  
Arjen de Boer ◽  
Johan Olsson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Fimbrial Adhesins

Fimbrial adhesins and mucosal inflammation

1990 ◽  
pp. 817-820
Author(s):  
H Linder ◽  
I Engberg ◽  
I Mattsby-Baltzer ◽  
K Jann ◽  
C Svanborg-Edén
Keyword(s):  
Mucosal Inflammation ◽  
Fimbrial Adhesins
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