Quantification of fenestrations in liver sinusoidal endothelial cells by atomic force microscopy

Micron ◽  
2017 ◽  
Vol 101 ◽  
pp. 48-53 ◽  
Author(s):  
Bartlomiej Zapotoczny ◽  
Karolina Szafranska ◽  
Edyta Kus ◽  
Stefan Chlopicki ◽  
Marek Szymonski
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy

2020 ◽  
Vol 12 (3) ◽  
pp. 625-636 ◽  
Author(s):  
Bartlomiej Zapotoczny ◽  
Filip Braet ◽  
Eddie Wisse ◽  
Malgorzata Lekka ◽  
Marek Szymonski
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Comparison of fixed and living liver endothelial cells by atomic force microscopy

1998 ◽  
Vol 66 (7) ◽  
pp. S575-S578 ◽  
Author(s):  
F. Braet ◽  
C. Rotsch ◽  
E. Wisse ◽  
M. Radmacher
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Liver Endothelial Cells

scholarly journals Biological Atomic Force Microscopy for Imaging Gold-Labeled Liposomes on Human Coronary Artery Endothelial Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ana-María Zaske ◽  
Delia Danila ◽  
Michael C. Queen ◽  
Eva Golunski ◽  
Jodie L. Conyers
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Coronary Artery ◽  
Cell Membrane ◽  
Fluorescent Microscopy ◽  
Human Coronary Artery ◽  
Force Microscopy ◽  
Cell Internalization ◽  
Atomic Force

Although atomic force microscopy (AFM) has been used extensively to characterize cell membrane structure and cellular processes such as endocytosis and exocytosis, the corrugated surface of the cell membrane hinders the visualization of extracellular entities, such as liposomes, that may interact with the cell. To overcome this barrier, we used 90 nm nanogold particles to label FITC liposomes and monitor their endocytosis on human coronary artery endothelial cells (HCAECs) in vitro. We were able to study the internalization process of gold-coupled liposomes on endothelial cells, by using AFM. We found that the gold-liposomes attached to the HCAEC cell membrane during the first 15–30 min of incubation, liposome cell internalization occurred from 30 to 60 min, and most of the gold-labeled liposomes had invaginated after 2 hr of incubation. Liposomal uptake took place most commonly at the periphery of the nuclear zone. Dynasore monohydrate, an inhibitor of endocytosis, obstructed the internalization of the gold-liposomes. This study showed the versatility of the AFM technique, combined with fluorescent microscopy, for investigating liposome uptake by endothelial cells. The 90 nm colloidal gold nanoparticles proved to be a noninvasive contrast agent that efficiently improves AFM imaging during the investigation of biological nanoprocesses.

Docking and fusion sites in human endothelial cells imaged and measured by using atomic force microscopy

2008 ◽  
Vol 13 (9) ◽  
pp. 580-580
Author(s):  
S. W. Schneider ◽  
R. Ossig ◽  
T. Görge ◽  
R. Matzke ◽  
P. Rogge ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Force Microscopy ◽  
Atomic Force
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