Silver nanoparticles modulate ABC transporter activity and enhance chemotherapy in multidrug resistant cancer

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

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Silver Nanoparticles and Their Antibacterial Applications

International Journal of Molecular Sciences ◽

10.3390/ijms22137202 ◽

2021 ◽

Vol 22 (13) ◽

pp. 7202

Author(s):

Tamara Bruna ◽

Francisca Maldonado-Bravo ◽

Paul Jara ◽

Nelson Caro

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Agents ◽

Multidrug Resistant ◽

Secondary Effects ◽

Cytotoxic Effects ◽

Factors Affecting ◽

Gram Positive Bacteria ◽

Resistant Strains

Silver nanoparticles (AgNPs) have been imposed as an excellent antimicrobial agent being able to combat bacteria in vitro and in vivo causing infections. The antibacterial capacity of AgNPs covers Gram-negative and Gram-positive bacteria, including multidrug resistant strains. AgNPs exhibit multiple and simultaneous mechanisms of action and in combination with antibacterial agents as organic compounds or antibiotics it has shown synergistic effect against pathogens bacteria such as Escherichia coli and Staphylococcus aureus. The characteristics of silver nanoparticles make them suitable for their application in medical and healthcare products where they may treat infections or prevent them efficiently. With the urgent need for new efficient antibacterial agents, this review aims to establish factors affecting antibacterial and cytotoxic effects of silver nanoparticles, as well as to expose the advantages of using AgNPs as new antibacterial agents in combination with antibiotic, which will reduce the dosage needed and prevent secondary effects associated to both.

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Pharmacological Properties of Biogenically Synthesized Silver Nanoparticles using Endophyte Bacillus cereus extract of Berberis lyceum against Oxidative Stress and Pathogenic Multidrug-Resistant Bacteria

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.07.009 ◽

2021 ◽

Author(s):

Neelam Mujaddidi ◽

Sobia Nisa ◽

Samha Al Ayoubi ◽

Yamin Bibi ◽

Salman Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Bacillus Cereus ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Pharmacological Properties ◽

Multidrug Resistant Bacteria

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The Synergistic Effect of Biosynthesized Silver Nanoparticles and Phage ZCSE2 as a Novel Approach to Combat Multidrug-Resistant Salmonella enterica

Antibiotics ◽

10.3390/antibiotics10060678 ◽

2021 ◽

Vol 10 (6) ◽

pp. 678

Author(s):

Abdallah S. Abdelsattar ◽

Rana Nofal ◽

Salsabil Makky ◽

Anan Safwat ◽

Amera Taha ◽

...

Keyword(s):

Silver Nanoparticles ◽

Color Change ◽

Antibacterial Properties ◽

Multidrug Resistant ◽

Inhibitory Effect ◽

Health Concern ◽

Resistant Bacteria ◽

Mortality And Morbidity ◽

Novel Approach ◽

Transmission Electron

The emergence and evolution of antibiotic-resistant bacteria is considered a public health concern. Salmonella is one of the most common pathogens that cause high mortality and morbidity rates in humans, animals, and poultry annually. In this work, we developed a combination of silver nanoparticles (AgNPs) with bacteriophage (phage) as an antimicrobial agent to control microbial growth. The synthesized AgNPs with propolis were characterized by testing their color change from transparent to deep brown by transmission electron microscopy (TEM) and Fourier-Transform Infrared Spectroscopy (FTIR). The phage ZCSE2 was found to be stable when combined with AgNPs. Both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated for AgNPs, phage, and their combination. The results indicated that MIC and MBC values were equal to 23 µg/mL against Salmonella bacteria at a concentration of 107 CFU/mL. The combination of 0.4× MIC from AgNPs and phage with Multiplicity of Infection (MOI) 0.1 showed an inhibitory effect. This combination of AgNPs and phage offers a prospect of nanoparticles with significantly enhanced antibacterial properties and therapeutic performance.

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Comparison of methods to detect the in vitro activity of silver nanoparticles (AgNP) against multidrug resistant bacteria

Journal of Nanobiotechnology ◽

10.1186/s12951-015-0120-6 ◽

2015 ◽

Vol 13 (1) ◽

Cited By ~ 86

Author(s):

Emerson Danguy Cavassin ◽

Luiz Francisco Poli de Figueiredo ◽

José Pinhata Otoch ◽

Marcelo Martins Seckler ◽

Roberto Angelo de Oliveira ◽

...

Keyword(s):

Silver Nanoparticles ◽

Multidrug Resistant ◽

Vitro Activity ◽

Resistant Bacteria ◽

Comparison Of Methods ◽

In Vitro Activity ◽

Multidrug Resistant Bacteria

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Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria

International Journal of Nanomedicine ◽

10.2147/ijn.s49284 ◽

2013 ◽

pp. 3187 ◽

Cited By ~ 124

Author(s):

Syed Zeeshan Naqvi ◽

Urooj Kiran ◽

Ali ◽

Jamal ◽

Hameed ◽

...

Keyword(s):

Silver Nanoparticles ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Multidrug Resistant Bacteria

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A REVIEW: A GREEN APPROACH FOR THE SYNTHESIS OF SILVER NANOPARTICLES AND ITS ANTIBACTERIAL APPLICATIONS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.26767 ◽

2018 ◽

Vol 11 (8) ◽

pp. 74 ◽

Cited By ~ 1

Author(s):

Shyla Marjorie Haqq ◽

Amit Chattree

Keyword(s):

Silver Nanoparticles ◽

Antimicrobial Properties ◽

Nanoparticle Synthesis ◽

Silver Ions ◽

Multidrug Resistant ◽

X Ray Diffraction ◽

Green Approach ◽

Transmission Electron ◽

Uv Visible ◽

Silver Nanoparticle Synthesis

This review is based on the synthesis of silver nanoparticles (AgNPs) using a green approach which is biofabricated from various medicinal plants. AgNPs were prepared from the various parts of the plants such as the flowers, stems, leaves, and fruits. Various physiochemical characterizations were performed using the ultraviolet (UV)-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and energy dispersive spectroscopy. AgNPs were also used to inhibit the growth of bacterial pathogens and were found to be effective against both the Gram-positive and Gram-negative bacteria. For the silver to have antimicrobial properties, it must be present in the ionized form. All the forms of silver-containing compounds with the observed antimicrobial properties are in one way or another source of silver ions. Although the antimicrobial properties of silver have been known, it is thought that the silver atoms bind to the thiol groups in enzymes and subsequently leads to the deactivation of enzymes. For the silver to have antimicrobial properties, it must be present in the ionized form. The study suggested that the action of the AgNPs on the microbial cells resulted into cell lysis and DNA damage. AgNPs have proved their candidature as a potential antibacterial against the multidrug-resistant microbes. The biological agents for synthesizing AgNPs cover compounds produced naturally in microbes and plants. Reaction parameters under which the AgNPs were being synthesized hold prominent impact on their size, shape, and application. Silver nanoparticle synthesis and their application are summarized and critically discussed in this review.

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