scholarly journals Accumulation of betacyanin in Hylocereus undatus rind: pigment stability analysis and its role in xanthine oxidase inhibition

2021 ◽  
pp. 100197
Author(s):  
Diksha Dey ◽  
Hridya Hemachandran ◽  
Thirumal Kumar D ◽  
George Priya Doss ◽  
Rossyda Priyadarshini ◽  
...  
Keyword(s):  
Stability Analysis ◽  
Xanthine Oxidase ◽  
Pigment Stability ◽  
Oxidase Inhibition ◽  
Hylocereus Undatus ◽  
Xanthine Oxidase Inhibition

Purpurogallin is a more powerful protector of kidney cells than Trolox and allopurinol

1992 ◽  
Vol 70 (8) ◽  
pp. 684-690 ◽  
Author(s):  
Ling-Hua Zeng ◽  
Tai-Wing Wu
Keyword(s):  
Xanthine Oxidase ◽  
Phase Contrast ◽  
Potent Inhibitor ◽  
Rat Kidney ◽  
Peroxyl Radicals ◽  
Kidney Cells ◽  
Tubular Epithelial Cells ◽  
Electron Microscopic ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

Phase contrast and electron microscopic experiments demonstrated that oxyradicals generated with xanthine oxidase and hypoxanthine markedly damage rat kidney mesangial and porcine tubular epithelial cells. Purpurogallin, a phenol found in oak nutgalls, prolongs survival of the xanthine oxidase exposed renal cells three- to nine-fold longer than those without purpurogallin present. At levels equimolar to purpurogallin, either Trolox or allopurinol is less effective in delaying cell necrosis. Purpurogallin scavenges not only xanthine oxidase generated oxyradicals, but also non-enzymatically produced peroxyl radicals, more actively than equimolar levels of Trolox or allopurinol. Purpurogallin inhibits xanthine oxidase with severalfold higher potency than allopurinol and its more active metabolite oxypurinol. Therefore, purpurogallin is a stronger antioxidant than Trolox and a more potent inhibitor of xanthine oxidase than allopurinol as well as oxypurinol.Key words: purpurogallin, kidney cells, oxyradical damage, xanthine oxidase inhibition, antioxidant.

scholarly journals Optimization of the Extraction Conditions and Biological Evaluation of Dendropanax morbifera H. Lev as an Anti-Hyperuricemic Source

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3313 ◽  
Author(s):  
Seung-Sik Cho ◽  
Seung-Hui Song ◽  
Chul-Yung Choi ◽  
Kyung Park ◽  
Jung-Hyun Shim ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Ethanol Extract ◽  
Oxidase Inhibitor ◽  
Xanthine Oxidase Inhibitor ◽  
Dendropanax Morbifera ◽  
Hexane Extracts ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition ◽  
Ethanol Extracts

Dendropanax morbifera H. Levis a medicinal plant native to South Korea, East Asia, and South America. Among some 75 species, one species grows in Korea. In previous studies, D. morbifera extracts with anti-oxidant, anti-inflammatory, anti-complementary and anti-cancer activities were reported. The present study aims to investigate optimization of extraction and evaluation of anti-hyperuricemic effects of D. morbifera leaf and the phytochemicals contained therein. Ethanol and hexane extract were found to display the best xanthine oxidase inhibition among six types of solvent and water extract. The antioxidant effect of the ethanol extract was superior to that of the hexane extract. The DPPH radical scavenging effect of the ethanol and hexane extracts were 81.52 ± 1.57% and 2.69 ± 0.16. The reducing power of the ethanol and hexane extracts were 9.71 ± 0.15 and 0.89 ± 0.01 mg/g equivalent of gallic acid. Total phenols of the ethanol and hexane extracts were 6.53 ± 0.16 and 0.63 ± 0.001 mg/g equivalent of gallic acid. In addition, we compared the two marker compounds from D. morbifera, chlorogenic acid and rutin, which were determined in the ethanol extract at 0.80 ± 0.03% and 0.52 ± 0.01%, respectively. We found that the ethanol extracts showed better xanthine oxidase inhibition than hexane extracts. Especially, ethanol extracts showed higher antioxidant activity than hexane extracts. Based on these results, we selected the ethanol extract as an effective xanthine oxidase inhibitor and confirmed whether ethanol extracts showed xanthine oxidase inhibition in animal experiments. The in vivo mouse study demonstrated that ethanol extract of D. morbifera leaf at the dose of 300 mg/kg could inhibit blood/hepatic xanthine oxidase activity and this result shows that the xanthine oxidase inhibitory activity in vitro is reproduced in vivo. The present study showed that ethanol extract was optimal xanthine oxidase inhibitor which can be applied to prevent diseases related to hyperuricemia.

Xanthine oxidase inhibition attenuates doxorubicin-induced cardiotoxicity in mice

2020 ◽  
Author(s):  
Yoshiro Tanaka ◽  
Tomohisa Nagoshi ◽  
Akira Yoshii ◽  
Yuhei Oi ◽  
Hirotake Takahashi ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

scholarly journals Antiremodeling Effect of Xanthine Oxidase Inhibition in a Canine Model of Atrial Fibrillation

2018 ◽  
Vol 59 (5) ◽  
pp. 1077-1085 ◽  
Author(s):  
Tomoharu Yoshizawa ◽  
Shinichi Niwano ◽  
Hiroe Niwano ◽  
Hideaki Tamaki ◽  
Hironori Nakamura ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Xanthine Oxidase ◽  
Canine Model ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

scholarly journals KAEMPFEROL FROM STELECHOCARPUS BURAHOL, (BL.) HOOK F. & TH. LEAVES AND XANTHINE OXIDASE INHIBITION ACTIVTY

2017 ◽  
Vol 10 (4) ◽  
pp. 322
Author(s):  
Diniatik K ◽  
Uwijiyo Pramono ◽  
Sugeng Riyanto
Keyword(s):  
Stationary Phase ◽  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Aqueous Ethanol ◽  
Oxidase Inhibitor ◽  
Xanthine Oxidase Inhibitor ◽  
Ic50 Values ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

Objective: Stelechocarpus burahol, (Bl.) Hook f. & Th. is a plant widely distributed in Java Island of Indonesia. The aim of this study is to identify compounds from the leaves of S. burahol that exhibited activity as xanthine oxidase inhibitor.Methods: The leaves were extracted with aqueous ethanol  and hidrolized with HCl methanol, then partitioned sequentially with chloroform and ethyl acetate. The ethyl acetate fractions were separated by coloumn chromatography with cellulose as stationary phase and methanol 50% as mobile phase.Results: Purification from this extracts afforded three compounds with one compound identified, namely Kaempferol. The four compounds possessed as xanthine oxidase inhibitor with IC50 values ranging from 0.27 to 0.45 μg/ml.Conclusion:Kaempferol exhibited the highest inhibition of 0.27 μg/ml.Keywords: Kaempferol, Xanthine Oxidase Inhibitor, Stelechocarpus burahol, (Bl.) Hook f. & Th. 

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