Non-selective cationic channels (NSCC) in smooth muscle cells from human umbilical arteries

Placenta ◽  
2004 ◽  
Vol 25 (8-9) ◽  
pp. 723-729 ◽  
Author(s):  
J. Raingo ◽  
A. Rebolledo ◽  
F. Iveli ◽  
A.O. Grassi de Gende ◽  
V. Milesi
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cationic Channels ◽  
Umbilical Arteries

Comparison of Platelet Interaction with Subendothelium of Human Renal and Umbilical Arteries and the Extracellular Matrix Produced by Human Venous Endothelial Cells

1984 ◽  
Vol 52 (01) ◽  
pp. 060-065 ◽  
Author(s):  
Kjell S Sakariassen ◽  
Jan Dirk Banga ◽  
Philip G de Groot ◽  
Jan J Sixma
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aggregate Formation ◽  
Disease Subtype ◽  
Elastic Membranes ◽  
Platelet Aggregate ◽  
Umbilical Arteries

SummaryPlatelet interaction with subendothelium of human renal and umbilical arteries and with the extracellular matrix produced by cultured human venous endothelial cells was compared in flowing citrated blood by using an annular and a rectangular perfusion chamber.The renal arteries were post mortem specimens from adults showing, often pronounced, intimal fibrosis, whereas the umbilical arteries had well organized parallel arranged smooth muscle cells, without elastic membranes. The extracellular matrix obtained after removal of endothelial cells with Triton X-100 was homogenously attached to its substratum.Significantly more platelets adhered to the extracellular matrix than to the subendothelia. This discrepancy was most pronounced in reconstituted blood with plasma from a patient with homozygous severe von Willebrand’s disease (subtype III). No differences in platelet adherence and platelet aggregate formation were noted between the subendothelia. Platelet aggregate formation was poor on all surfaces.These data indicate that the extracellular matrix produced by endothelial cells is at least as reactive for the interaction with platelets as subendothelium, probably partly synthesized by smooth muscle cells.

Hyperpolarization-activated cationic channels in smooth muscle cells are stretch sensitive

1991 ◽  
Vol 417 (5) ◽  
pp. 493-499 ◽  
Author(s):  
Tetsuya Hisada ◽  
Richard W. Ordway ◽  
Michael T. Kirber ◽  
Joshua J. Singer ◽  
John V. Walsh
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cationic Channels

Stretch-inactivated cationic channels in single smooth muscle cells

1993 ◽  
Vol 422 (4) ◽  
pp. 393-396 ◽  
Author(s):  
Tetsuya Hisada ◽  
John V. Walsh ◽  
Joshua J. Singer
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cationic Channels

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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