scholarly journals Targeting Acyl Homoserine Lactones (AHLs) by the Quorum Quenching Bacterial Strains to Control Biofilm Formation in Pseudomonas Aeruginosa

2021 ◽  
Author(s):  
Javariya Shirazi ◽  
Quratul Ain ◽  
Sher Jamal Khan ◽  
Amna Jalil ◽  
Muhammad Faisal Siddiqui ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Quorum Quenching ◽  
Bacterial Strains ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 225
Author(s):  
Xiaohui Sun ◽  
Philip Hill ◽  
Jia Liu ◽  
Jing Qian ◽  
Yuting Ma ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Quorum Quenching ◽  
Bacillus Velezensis ◽  
Antifouling Activity ◽  
Homoserine Lactones ◽  
Water Filter ◽  
E Coli ◽  
Acyl Homoserine Lactones ◽  
Dental Practitioners ◽  
Marine Source

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the improvement of hygiene problems caused by biofilm infection of Pseudomonas aeruginosa in dental units. Pseudomonas bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of P. aeruginosa, and was efficient on the antifouling against P. aeruginosa. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units.

scholarly journals Biofilm Formation by Marine Cobetia marina alex and Pseudoalteromonas spp: Development and Detection of Quorum Sensing N-Acyl Homoserine Lactones (AHLs) Molecules

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Samia S. Abouelkheir ◽  
Eman A. Abdelghany ◽  
Soraya A. Sabry ◽  
Hanan A. Ghozlan
Keyword(s):  
Biofilm Formation ◽  
Signal Molecules ◽  
Bacterial Strains ◽  
Factors Affecting ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones ◽  
Single Culture ◽  
Cobetia Marina ◽  
Selection For ◽  
Antagonistic Interactions

Surfaces submerged in seawater are colonized by various microorganisms, resulting in the formation of heterogenic marine biofilms. This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater, Alexandria, Egypt. Three strains; Cobetia marina alex, Pseudoalteromonas sp. alex, and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet (CV) quantification method in a single culture. The values of biofilm formed were OD600= 3.0, 2.7, and 2.6, respectively leading to their selection for further evaluation. However, factors affecting biofilm formation by C. marina alex were investigated. Biofilm formation was evaluated in single and multispecies consortia. Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones (AHLs)

Inhibition of biofilm formation of Pseudomonas aeruginosa by an acylated homoserine lactones-containing culture extract

LWT ◽  
2014 ◽  
Vol 57 (1) ◽  
pp. 230-235 ◽  
Author(s):  
Qiu Qin Zhang ◽  
Ke Ping Ye ◽  
Hu Hu Wang ◽  
Hong Mei Xiao ◽  
Xing Lian Xu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Homoserine Lactones ◽  
Culture Extract ◽  
Acylated Homoserine Lactones

scholarly journals Insights from the Genome Sequence of Quorum-Quenching Staphylococcus sp. Strain AL1, Isolated from Traditional Chinese Soy Sauce Brine Fermentation

2012 ◽  
Vol 194 (23) ◽  
pp. 6611-6612 ◽  
Author(s):  
Teik-Min Chong ◽  
Hun-Jiat Tung ◽  
Wai-Fong Yin ◽  
Kok-Gan Chan
Keyword(s):  
Quorum Sensing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Quorum Quenching ◽  
Soy Sauce ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

ABSTRACTWe report the draft genome sequence ofStaphylococcussp. strain AL1, which degrades quorum-sensing molecules (namely,N-acyl homoserine lactones). To the best of our knowledge, this is the first documentation that reports the whole genome sequence and quorum-quenching activity ofStaphylococcussp. strain AL1.

scholarly journals Haloperoxidase Mediated Quorum Quenching by Nitzschia cf pellucida: Study of the Metabolization of N-Acyl Homoserine Lactones by a Benthic Diatom

Marine Drugs ◽  
2014 ◽  
Vol 12 (1) ◽  
pp. 352-367 ◽  
Author(s):  
Michail Syrpas ◽  
Ewout Ruysbergh ◽  
Lander Blommaert ◽  
Bart Vanelslander ◽  
Koen Sabbe ◽  
...  
Keyword(s):  
Quorum Quenching ◽  
Benthic Diatom ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals Effects of Exogenous N-Acyl-Homoserine Lactones on Biofilm Formation and Motility in Acinetobacter nosocomialis

2020 ◽  
Vol 50 (2) ◽  
pp. 97-106
Author(s):  
Surya Surendran ◽  
Bindu Subhadra ◽  
Kyungho Woo ◽  
Ho Sung Park ◽  
Dong Ho Kim ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals Evolution of the quorum sensing regulon in cooperating populations of Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Nicole E Smalley ◽  
Amy L Schaefer ◽  
Kyle L Asfahl ◽  
Crystal Perez ◽  
E Peter Greenberg ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Cell Communication ◽  
Opportunistic Pathogen ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Rna Seq ◽  
Bacterial Strains ◽  
Communication And Coordination

The bacterium Pseudomonas aeruginosa is an opportunistic pathogen and it thrives in many different saprophytic habitats. In this bacterium acyl-homoserine lactone quorum sensing (QS) can activate expression of over 100 genes, many of which code for extracellular products. P. aeruginosa has become a model for studies of cell-cell communication and coordination of cooperative activities. We hypothesized that long-term growth of bacteria under conditions where only limited QS-controlled functions were required would result in a reduction in the size of the QS-controlled regulon. To test this hypothesis, we grew P. aeruginosa for about 1000 generations in a condition in which expression of QS-activated genes is required for growth. We compared the QS regulons of populations after about 35 generations to those after about 1000 generations in two independent lineages by using quorum quenching and RNA-seq technology. In one evolved lineage the number of QS-activated genes identified was reduced by about 70% and in the other by about 45%. Our results lend important insights about the variations in the number of QS-activated genes reported for different bacterial strains and, more broadly, about the environmental histories of P. aeruginosa.

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