A signal amplification strategy based on peptide self-assembly for the identification of amyloid-β oligomer

2021 ◽  
pp. 129697
Author(s):  
Yujin Huang ◽  
Baole Zhang ◽  
Liang Yuan ◽  
Lei Liu
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Amyloid Β ◽  
Amplification Strategy

A Feasible Self-assembly Near-Infrared Fluorescence Sensor for Acid Phosphatase Detection and Cell Imaging

The Analyst ◽  
2021 ◽  
Author(s):  
Shuangqin Li ◽  
Yaya Wang ◽  
Shuai Mu ◽  
Jinlong Zhang ◽  
Xiaoyan Liu ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Self Assembly ◽  
Signal Amplification ◽  
Near Infrared ◽  
Cell Imaging ◽  
Near Infrared Fluorescence ◽  
Disease Biomarkers ◽  
Amplification Strategy ◽  
Multi Mode ◽  
Limited Accuracy

Abstract Single signal amplification strategy is significant for detecting of various disease biomarkers, however, which is restricted by the limited accuracy. The multi-signal and multi-mode methods have efficiently overcome this...

Hairpin DNA-fueled dynamic self-assembly of three-arm DNA branched junctions consisting of active DNAzyme structures for enzyme-free ultrasensitive detection of nucleic acids

2016 ◽  
Vol 8 (47) ◽  
pp. 8262-8265 ◽  
Author(s):  
Ke Yang ◽  
Ming Zeng ◽  
Xing He ◽  
Jianming Li ◽  
Dinggeng He
Keyword(s):  
Nucleic Acids ◽  
Self Assembly ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Hairpin Dna ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An enzyme-free dual signal amplification strategy based on programmable molecular hairpins has been developed for amplified detection of DNA via the hairpin DNA-fueled dynamic self-assembly of three-arm DNAzyme.

scholarly journals An evaluation of the self-assembly enhancing properties of cell-derived hexameric amyloid-β

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Devkee M. Vadukul ◽  
Céline Vrancx ◽  
Pierre Burguet ◽  
Sabrina Contino ◽  
Nuria Suelves ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Self Assembly ◽  
Critical Nucleus ◽  
Amyloid Fibril ◽  
Amyloid Β ◽  
Amyloid Plaques ◽  
The Self ◽  
Aβ Peptide ◽  
Amyloid Fibril Formation ◽  
Secretase Activity

AbstractA key hallmark of Alzheimer’s disease is the extracellular deposition of amyloid plaques composed primarily of the amyloidogenic amyloid-β (Aβ) peptide. The Aβ peptide is a product of sequential cleavage of the Amyloid Precursor Protein, the first step of which gives rise to a C-terminal Fragment (C99). Cleavage of C99 by γ-secretase activity releases Aβ of several lengths and the Aβ42 isoform in particular has been identified as being neurotoxic. The misfolding of Aβ leads to subsequent amyloid fibril formation by nucleated polymerisation. This requires an initial and critical nucleus for self-assembly. Here, we identify and characterise the composition and self-assembly properties of cell-derived hexameric Aβ42 and show its assembly enhancing properties which are dependent on the Aβ monomer availability. Identification of nucleating assemblies that contribute to self-assembly in this way may serve as therapeutic targets to prevent the formation of toxic oligomers.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Naked-Eye Detection of Hg(II) Ions by Visible Light-Induced Polymerization Initiated by a Hg(II)-Selective Photoredox Catalyst

2021 ◽  
Author(s):  
Hyungwook Kim ◽  
Young Jae Jung ◽  
Jungkyu K. Lee
Keyword(s):  
Visible Light ◽  
Signal Amplification ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Novel Strategy ◽  
Amplification Strategy ◽  
Naked Eye Detection

We developed a novel strategy for signal amplification strategy using a visible light-induced photopolymerization, initiated by a selective turn-on photoredox catalyst. As photoredox catalysts, fluorescein derivatives are able to initiate...

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

Dual-signal amplification strategy for electrochemiluminescence sandwich biosensor for detection of thrombin

2017 ◽  
Vol 240 ◽  
pp. 742-748 ◽  
Author(s):  
Yingjie Li ◽  
Yuqin Li ◽  
Ning Xu ◽  
Jiahong Pan ◽  
Tufeng Chen ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Amplification Strategy ◽  
Dual Signal Amplification
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