scholarly journals Chitinolytic activity of phylogenetically diverse Bacillus cereus sensu lato from natural environments

2020 ◽  
Vol 43 (3) ◽  
pp. 126075 ◽  
Author(s):  
J.M. Drewnowska ◽  
A. Fiodor ◽  
J.E. Barboza-Corona ◽  
I. Swiecicka
Keyword(s):  
Bacillus Cereus ◽  
Natural Environments ◽  
Chitinolytic Activity ◽  
Bacillus Cereus Sensu Lato

Pathogenicity and characterization of a novel Bacillus cereus sensu lato isolate toxic to the Mediterranean fruit fly Ceratitis capitata Wied.

2015 ◽  
Vol 126 ◽  
pp. 71-77 ◽  
Author(s):  
Luca Ruiu ◽  
Giovanni Falchi ◽  
Ignazio Floris ◽  
Maria Giovanna Marche ◽  
Maria Elena Mura ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean ◽  
Bacillus Cereus Sensu Lato

Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03

2020 ◽  
Author(s):  
Estefanía Morales-Ruiz ◽  
Ricardo Priego-Rivera ◽  
Alejandro Miguel Figueroa-López ◽  
Jesús Eduardo Cazares-Álvarez ◽  
Ignacio E Maldonado-Mendoza
Keyword(s):  
Bacillus Cereus ◽  
Biochemical Characterization ◽  
Fusarium Verticillioides ◽  
Expression System ◽  
Phytopathogenic Fungi ◽  
Fungal Cell ◽  
Synthetic Pesticides ◽  
Bacillus Cereus Sensu Lato ◽  
Dual Substrate

Abstract Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides.

scholarly journals Diversity of Bacillus cereus sensu lato mobilome

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Nancy Fayad ◽  
Mireille Kallassy Awad ◽  
Jacques Mahillon
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Cereus Sensu Lato

scholarly journals Comparative Transcriptional Profiling of Bacillus cereus Sensu Lato Strains during Growth in CO2-Bicarbonate and Aerobic Atmospheres

PLoS ONE ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. e4904 ◽  
Author(s):  
Karla D. Passalacqua ◽  
Anjana Varadarajan ◽  
Benjamin Byrd ◽  
Nicholas H. Bergman
Keyword(s):  
Bacillus Cereus ◽  
Transcriptional Profiling ◽  
Bacillus Cereus Sensu Lato

scholarly journals Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis

2012 ◽  
Vol 22 (8) ◽  
pp. 1512-1524 ◽  
Author(s):  
M. E. Zwick ◽  
S. J. Joseph ◽  
X. Didelot ◽  
P. E. Chen ◽  
K. A. Bishop-Lilly ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genomic Characterization ◽  
Bacillus Cereus Sensu Lato

scholarly journals Cereulide synthetase acquisition and loss events within the evolutionary history of Group III Bacillus cereus sensu lato facilitate the transition between emetic and diarrheal foodborne pathogen

2020 ◽  
Author(s):  
Laura M. Carroll ◽  
Martin Wiedmann
Keyword(s):  
Bacillus Cereus ◽  
Evolutionary History ◽  
Common Ancestor ◽  
Reference Genome ◽  
Foodborne Pathogen ◽  
Genomic Diversity ◽  
Whole Genome ◽  
Group Iii ◽  
History Of ◽  
Bacillus Cereus Sensu Lato

AbstractCereulide-producing members of Bacillus cereus sensu lato (B. cereus s.l.) Group III, also known as “emetic B. cereus”, possess cereulide synthetase, a plasmid-encoded, non-ribosomal peptide synthetase encoded by the ces gene cluster. Despite the documented risks that cereulide-producing strains pose to public health, the level of genomic diversity encompassed by “emetic B. cereus” has never been evaluated at a whole-genome scale. Here, we employ a phylogenomic approach to characterize Group III B. cereus s.l. genomes which possess ces (ces-positive) alongside their closely related ces-negative counterparts to (i) assess the genomic diversity encompassed by “emetic B. cereus”, and (ii) identify potential ces loss and/or gain events within the evolutionary history of the high-risk and medically relevant sequence type (ST) 26 lineage often associated with emetic foodborne illness. Using all publicly available ces-positive Group III B. cereus s.l. genomes and the ces-negative genomes interspersed among them (n = 150), we show that “emetic B. cereus” is not clonal; rather, multiple lineages within Group III harbor cereulide-producing strains, all of which share a common ancestor incapable of producing cereulide (posterior probability [PP] 0.86-0.89). The ST 26 common ancestor was predicted to have emerged as ces-negative (PP 0.60-0.93) circa 1904 (95% highest posterior density [HPD] interval 1837.1-1957.8) and first acquired the ability to produce cereulide before 1931 (95% HPD 1893.2-1959.0). Three subsequent ces loss events within ST 26 were observed, including among isolates responsible for B. cereus s.l. toxicoinfection (i.e., “diarrheal” illness).Importance“B. cereus” is responsible for thousands of cases of foodborne disease each year worldwide, causing two distinct forms of illness: (i) intoxication via cereulide (i.e., “emetic” syndrome) or (ii) toxicoinfection via multiple enterotoxins (i.e., “diarrheal” syndrome). Here, we show that “emetic B. cereus” is not a clonal, homogenous unit that resulted from a single cereulide synthetase gain event followed by subsequent proliferation; rather, cereulide synthetase acquisition and loss is a dynamic, ongoing process that occurs across lineages, allowing some Group III B. cereus s.l. populations to oscillate between diarrheal and emetic foodborne pathogen over the course of their evolutionary histories. We also highlight the care that must be taken when selecting a reference genome for whole-genome sequencing-based investigation of emetic B. cereus s.l. outbreaks, as some reference genome selections can lead to a confounding loss of resolution and potentially hinder epidemiological investigations.

scholarly journals Effect of infant meal home preparation temperature on surviving of Bacillus cereus sensu lato: A case of Bechar city, Algeria

2021 ◽  
Vol 25 (2) ◽  
pp. 201-210
Author(s):  
Hassiba Difallah ◽  
Mohammed Ziane ◽  
Olfa Ben Braïek ◽  
Mohammed Bouamra ◽  
Habiba Louzim
Keyword(s):  
Heat Resistance ◽  
Bacillus Cereus ◽  
Storage Conditions ◽  
The Other ◽  
Preparation Temperature ◽  
Meal Preparation ◽  
Production Preparation ◽  
And Storage ◽  
Bacterial Concentrations ◽  
Bacillus Cereus Sensu Lato

Abstract This work aimed to enumerate the Bacillus cereus sensu lato from infant’s flour sampled at Béchar city and evaluate its resistance to different heating conditions during meal preparation patterns at home. Our findings revealed a prevalence of 74% with 2.4 to 3.9 CFU/g in the analyzed samples. Regarding the heat resistance at 90 °C to 98 °C, our results showed heat resistance variability which depends on the isolate, for example, D90 °C and zT °C values varied from 3.24 to 5.52 min and 11.56 to 89.74 °C respectively. Then, the decimal reduction (n) was calculated at all preparation temperatures (50, 60, 70, 80, 90 and 100 °C). Low “n” was observed with the preparation at T≤50 °C as recommended by the fabricant. However, at the other temperatures, high “n” was observed at 100°C with median and 95th values of 2.22 and 12.36 respectively. Therefore, bacterial concentrations (99th) were estimated at 0.124 log CFU/g for 100 °C. These concentrations could be increased with bacterial growth during meal storage and then achieve critical concentrations. Thus, the results of this work highlight the interest to establish a risk assessment for babies and to improve the production, preparation, and storage conditions of the infant’s flour.

scholarly journals Genomic Insights into the Diversity of Non-Coding RNAs in Bacillus Cereus Sensu Lato.

2021 ◽  
Author(s):  
Kátia B. Gonçalves ◽  
Renan J. Casarotto Appel ◽  
Laurival A. Vilas Boas ◽  
Priscilla F. Cardoso ◽  
Gislayne T. Vilas Boas
Keyword(s):  
Bacillus Cereus ◽  
Class Group ◽  
Environmental Changes ◽  
Wide Distribution ◽  
Ecological Niches ◽  
Rt Pcr ◽  
Non Coding Rnas ◽  
Structural Category ◽  
Bacterial Groups ◽  
Bacillus Cereus Sensu Lato

Abstract Bacillus cereus sensu lato is a group of bacteria of medical and agricultural importance in different ecological niches and with controversial taxonomic relationships. Studying the composition of non-coding RNAs (ncRNAs) in several bacterial groups has been an important tool for identifying genetic information. However, to date, no comparative genomics study of ncRNA has been performed in this group. Thus, this study aimed to identify and characterize the set of ncRNAs from 132 strains of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis to obtain an overview of the diversity and distribution of these genetic elements in these species. We observed that the number of ncRNAs differs in the chromosomes of the three species, but not in the plasmids, when species or phylogenetic clusters were compared. The prevailing functional/structural category was Cis-reg and the most frequent class was Riboswitch. However, in plasmids, the class Group II intron was the most frequent. Also, nine ncRNAs were selected for validation in the strain B. thuringiensis 407 by RT-PCR, which allowed to identify the expression of the ncRNAs. The wide distribution and diversity of ncRNAs in the B. cereus group, and more intensely in B. thuringiensis, may help improve the abilities of these species to adapt to various environmental changes.

scholarly journals TAXONOMY, VIRULENCE AND LIFE CYCLES OF BACILLUS CEREUS SENSU LATO

2019 ◽  
Vol 56 (4) ◽  
pp. 440-450
Author(s):  
Marek Bartoszewicz ◽  
Urszula Czyżewska
Keyword(s):  
Bacillus Cereus ◽  
Life Cycles ◽  
Bacillus Cereus Sensu Lato

scholarly journals Potential Enterotoxicity of Phylogenetically Diverse Bacillus cereus Sensu Lato Soil Isolates from Different Geographical Locations

2020 ◽  
Vol 86 (11) ◽  
Author(s):  
Justyna Malgorzata Drewnowska ◽  
Natalia Stefanska ◽  
Magdalena Czerniecka ◽  
Grzegorz Zambrowski ◽  
Izabela Swiecicka
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Population ◽  
Phylogenetic Groups ◽  
Content Type ◽  
Hot Climate ◽  
Hemolysin Bl ◽  
Spore Forming Bacteria ◽  
Geographical Locations ◽  
Bacillus Cereus Sensu Lato

ABSTRACT Bacillus cereus sensu lato comprises Gram-positive spore-forming bacteria producing toxins associated with foodborne diseases. Three pore-forming enterotoxins, nonhemolytic enterotoxin (Nhe), hemolysin BL (Hbl), and cytotoxin K (CytK), are considered the primary factors in B. cereus sensu lato diarrhea. The aim of this study was to determine the potential risk of enterotoxicity among soil B. cereus sensu lato isolates representing diverse phylogroups and originated from different geographic locations with various climates (Burkina Faso, Kenya, Argentina, Kazakhstan, and Poland). While nheA- and hblA-positive isolates were present among all B. cereus sensu lato populations and distributed across all phylogenetic groups, cytK-2-positive strains predominated in geographic regions with an arid hot climate (Africa) and clustered together on a phylogenetic tree mainly within mesophilic groups III and IV. The highest in vitro cytotoxicity to Caco-2 and HeLa cells was demonstrated by the strains clustered within phylogroups II and IV. Overall, our results suggest that B. cereus sensu lato pathogenicity is a comprehensive process conditioned by many intracellular factors and diverse environmental conditions. IMPORTANCE This research offers a new route for a wider understanding of the dependency between pathogenicity and phylogeny of a natural bacterial population, specifically within Bacillus cereus sensu lato, that is widely distributed around the world and easily transferred into food products. Our study indicates differences in the phylogenetic and geographical distributions of potential enterotoxigenic B. cereus sensu lato strains. Hence, these bacilli possess a risk for human health, and rapid testing methods for their identification are greatly needed. In particular, the detection of the CytK enterotoxin should be a supporting strategy for the identification of pathogenic B. cereus sensu lato.

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