Isolation and characterization of goat ovarian aromatase cDNA: assessment of the activity using an intact cell system and placental expression

2004 ◽  
Vol 62 (3-4) ◽  
pp. 532-543 ◽  
Author(s):  
Raúl José Bobes ◽  
Carolina Miranda ◽  
Mario Pérez-Martinez ◽  
Van Luu-The ◽  
Marta C Romano
Keyword(s):  
Intact Cell ◽  
Cell System ◽  
Isolation And Characterization

Isolation and characterization of the components of the sodium pump

1974 ◽  
Vol 7 (2) ◽  
pp. 239-274 ◽  
Author(s):  
Peter Leth Jørgensen
Keyword(s):  
Cell Membrane ◽  
Active Transport ◽  
Transport System ◽  
Sodium Pump ◽  
Enzyme System ◽  
Intact Cell ◽  
Test Tube ◽  
Isolation And Characterization ◽  
Sodium And Potassium

A satisfactory understanding of the functions of the sodium pump, the system responsible for the active transport of sodium and potassium, require the isolation and characterization of its protein and lipid components which are integrated in the structure of the cell membrane. The enzyme system (Na+ + K+)-ATPase, is located in membrane fragments and behaves in the test tube like the transport system in the intact cell membrane (Skou,1957) Purified preparations of this enzyme will contain some, if not all, of the components of the sodium pump.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

Isolation and characterization of new phenanthrenes from Juncus inflexus and their chemotaxonomic significance

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
B Tóth ◽  
N Kúsz ◽  
A Csorba ◽  
T Kurtán ◽  
J Hohmann ◽  
...  
Keyword(s):  
Isolation And Characterization

Isolation and characterization of a novel O-methyltransferase from the tropical liana Triphyophyllum peltatum

2017 ◽  
Author(s):  
L Passolt ◽  
A Jindaprasert ◽  
T Le Tran ◽  
R Seupel ◽  
G Bringmann ◽  
...  
Keyword(s):  
Isolation And Characterization

Isolation and Characterization of Plasminogen Activator from Pig Leucocytes

1974 ◽  
Vol 31 (01) ◽  
pp. 072-085 ◽  
Author(s):  
M Kopitar ◽  
M Stegnar ◽  
B Accetto ◽  
D Lebez
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Ph Optimum ◽  
Isolation And Characterization ◽  
Ph Range ◽  
Inhibition Studies ◽  
Final Purification

SummaryPlasminogen activator was isolated from disrupted pig leucocytes by the aid of DEAE chromatography, gel filtration on Sephadex G-100 and final purification on CM cellulose, or by preparative gel electrophoresis.Isolated plasminogen activator corresponds No. 3 band of the starting sample of leucocyte cells (that is composed from 10 gel electrophoretic bands).pH optimum was found to be in pH range 8.0–8.5 and the highest pH stability is between pH range 5.0–8.0.Inhibition studies of isolated plasminogen activator were performed with EACA, AMCHA, PAMBA and Trasylol, using Anson and Astrup method. By Astrup method 100% inhibition was found with EACA and Trasylol and 30% with AMCHA. PAMBA gave 60% inhibition already at concentration 10–3 M/ml. Molecular weight of plasminogen activator was determined by gel filtration on Sephadex G-100. The value obtained from 4 different samples was found to be 28000–30500.

Sign in / Sign up

Export Citation Format

Share Document