scholarly journals Transfusion-Induced Bone Marrow Transplant Rejection Due to Minor Histocompatibility Antigens

2013 ◽  
Vol 27 (4) ◽  
pp. 241-248 ◽  
Author(s):  
Seema R. Patel ◽  
James C. Zimring
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplant ◽  
Transplant Rejection ◽  
Marrow Transplant ◽  
Histocompatibility Antigens ◽  
Minor Histocompatibility Antigens

scholarly journals Graft-host tolerance in bone marrow transplant chimeras. Absence of graft-versus-host disease is associated with unresponsiveness to minor histocompatibility antigens expressed by all tissues

Blood ◽  
1994 ◽  
Vol 84 (9) ◽  
pp. 3221-3228
Author(s):  
S Brochu ◽  
C Baron ◽  
R Belanger ◽  
C Perreault
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
T Lymphocytes ◽  
Marrow Transplant ◽  
Lymphoid Cells ◽  
Histocompatibility Antigens ◽  
Minor Histocompatibility Antigens ◽  
Cd8 Cells ◽  
B10 Cells

Because bone marrow (BM) transplantation is used with increasing frequency, it is important to elucidate the mechanisms involved in the establishment of tolerance to host minor histocompatibility antigens (MiHA) in recipients transplanted with T-cell-undepleted marrow grafts. We have previously shown that BM chimeras transplanted across MiHA barriers showed specific unresponsiveness to MiHA expressed on recipient-type concanavalin A blasts. Because expression of many MiHA is tissue-specific, we wanted to determine if chimera T lymphocytes would be tolerant to MiHA expressed by all host tissues and organs. To investigate this issue, we measured in vivo proliferation of lymphoid cells from normal C57BL/10 (B10) mice and (B10-->LP) chimeras in tissues and organs of lethally irradiated syngeneic and allogeneic recipients. Donor B10 cells were either untreated, or depleted with anti-Thy-1.2, anti-CD4, or anti-CD8 antibodies. Transplantation of B10 cells in LP recipients triggered an important T-cell-dependent 125I- dUrd uptake in several organs that involved both CD4+ and CD8+ cells. Using Thy-1-congeneic mice we showed that in long-term chimeras practically all CD4+ and CD8+ T lymphocytes were derived from hematopoietic progenitors and not from mature T cells present in the BM graft. When (B10-->LP) BM chimera cells were injected to secondary recipients, no proliferation was observed in any organ of LP hosts whereas normal proliferation was seen in H-2k allogeneic hosts. Thus, in these BM chimeras, tolerance encompasses MiHA expressed by all organs.

scholarly journals Successful use of multiagent immunosuppression in the bone marrow transplantation of sensitized patients

Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1163-1169 ◽  
Author(s):  
R Parkman ◽  
J Rappeport ◽  
B Camitta ◽  
RH Levey ◽  
DG Nathan
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Aplastic Anemia ◽  
Bone Marrow Transplant ◽  
Marrow Transplantation ◽  
Marrow Transplant ◽  
Severe Aplastic Anemia ◽  
Transplant Recipients ◽  
Histocompatibility Antigens

Abstract Of 23 patients with severe aplastic anemia, 17 were sensitized to histocompatibility antigens of HLA-A, -B, and -D loci-identical potential sibling donors as determined by cell-mediated lysis (CML) assays in vitro. Antibody-dependent sensitization was detected in 3 patients, antibody-independent cellular sensitization in 11, and both in 3. Fourteen sensitized patients were transplanted after initial multiagent immunosuppression consisting of rabbit anti-human thymocyte serum, procarbazine, and cyclophosphamide, eleven with a CML-positive donor and three with a CML-negative donor. Engraftment was achieved in each of 13 patients who were evaluable, and only 2 ultimately rejected their marrow grafts, 1 with subsequent return of his own marrow function. Five patients without evidence in vitro of sensitization were transplanted after immunosuppression with cyclophosphamide alone; none of these rejected their grafts. These studies show that sensitized bone marrow transplant recipients can be successfully transplanted after optimal donor selection and multiagent immunosuppression.

Graft-host tolerance in bone marrow transplant chimeras. Absence of graft-versus-host disease is associated with unresponsiveness to minor histocompatibility antigens expressed by all tissues

Blood ◽  
1994 ◽  
Vol 84 (9) ◽  
pp. 3221-3228 ◽  
Author(s):  
S Brochu ◽  
C Baron ◽  
R Belanger ◽  
C Perreault
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
T Lymphocytes ◽  
Marrow Transplant ◽  
Lymphoid Cells ◽  
Histocompatibility Antigens ◽  
Minor Histocompatibility Antigens ◽  
Cd8 Cells ◽  
B10 Cells

Abstract Because bone marrow (BM) transplantation is used with increasing frequency, it is important to elucidate the mechanisms involved in the establishment of tolerance to host minor histocompatibility antigens (MiHA) in recipients transplanted with T-cell-undepleted marrow grafts. We have previously shown that BM chimeras transplanted across MiHA barriers showed specific unresponsiveness to MiHA expressed on recipient-type concanavalin A blasts. Because expression of many MiHA is tissue-specific, we wanted to determine if chimera T lymphocytes would be tolerant to MiHA expressed by all host tissues and organs. To investigate this issue, we measured in vivo proliferation of lymphoid cells from normal C57BL/10 (B10) mice and (B10-->LP) chimeras in tissues and organs of lethally irradiated syngeneic and allogeneic recipients. Donor B10 cells were either untreated, or depleted with anti-Thy-1.2, anti-CD4, or anti-CD8 antibodies. Transplantation of B10 cells in LP recipients triggered an important T-cell-dependent 125I- dUrd uptake in several organs that involved both CD4+ and CD8+ cells. Using Thy-1-congeneic mice we showed that in long-term chimeras practically all CD4+ and CD8+ T lymphocytes were derived from hematopoietic progenitors and not from mature T cells present in the BM graft. When (B10-->LP) BM chimera cells were injected to secondary recipients, no proliferation was observed in any organ of LP hosts whereas normal proliferation was seen in H-2k allogeneic hosts. Thus, in these BM chimeras, tolerance encompasses MiHA expressed by all organs.

scholarly journals Successful use of multiagent immunosuppression in the bone marrow transplantation of sensitized patients

Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1163-1169 ◽  
Author(s):  
R Parkman ◽  
J Rappeport ◽  
B Camitta ◽  
RH Levey ◽  
DG Nathan
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Aplastic Anemia ◽  
Bone Marrow Transplant ◽  
Marrow Transplantation ◽  
Marrow Transplant ◽  
Severe Aplastic Anemia ◽  
Transplant Recipients ◽  
Histocompatibility Antigens

Of 23 patients with severe aplastic anemia, 17 were sensitized to histocompatibility antigens of HLA-A, -B, and -D loci-identical potential sibling donors as determined by cell-mediated lysis (CML) assays in vitro. Antibody-dependent sensitization was detected in 3 patients, antibody-independent cellular sensitization in 11, and both in 3. Fourteen sensitized patients were transplanted after initial multiagent immunosuppression consisting of rabbit anti-human thymocyte serum, procarbazine, and cyclophosphamide, eleven with a CML-positive donor and three with a CML-negative donor. Engraftment was achieved in each of 13 patients who were evaluable, and only 2 ultimately rejected their marrow grafts, 1 with subsequent return of his own marrow function. Five patients without evidence in vitro of sensitization were transplanted after immunosuppression with cyclophosphamide alone; none of these rejected their grafts. These studies show that sensitized bone marrow transplant recipients can be successfully transplanted after optimal donor selection and multiagent immunosuppression.

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