Peritransplant and Long-Term Secretion of Interleukin-1β in Cyclosporine Treated Syngeneic Rats Allografted With Islets of Langerhans

2005 ◽  
Vol 37 (5) ◽  
pp. 2375-2378 ◽  
Author(s):  
A.O. Gürol ◽  
A.Ö. Kurşun ◽  
F. Süzergöz ◽  
U.C. Küçüksezer ◽  
B. Kıran ◽  
...  
Keyword(s):  
Islets Of Langerhans ◽  
Interleukin 1Β

Long-term cryogenic storage of purified adult human islets of Langerhans

Diabetes ◽  
1989 ◽  
Vol 38 (3) ◽  
pp. 386-396 ◽  
Author(s):  
N. M. Kneteman ◽  
D. Alderson ◽  
D. W. Scharp ◽  
P. E. Lacy
Keyword(s):  
Islets Of Langerhans ◽  
Human Islets ◽  
Cryogenic Storage ◽  
Adult Human ◽  
Human Islets Of Langerhans

scholarly journals Long-term treatment with interleukin-1β induces insulin resistance in murine and human adipocytes

Diabetologia ◽  
2006 ◽  
Vol 49 (9) ◽  
pp. 2162-2173 ◽  
Author(s):  
C. Lagathu ◽  
L. Yvan-Charvet ◽  
J.-P. Bastard ◽  
M. Maachi ◽  
A. Quignard-Boulangé ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Term Treatment ◽  
Interleukin 1Β ◽  
Human Adipocytes ◽  
Long Term Treatment

scholarly journals Altered proinsulin conversion in rat pancreatic islets exposed long-term to various glucose concentrations or interleukin-1β

2007 ◽  
Vol 192 (2) ◽  
pp. 381-387 ◽  
Author(s):  
Andreas Börjesson ◽  
Carina Carlsson
Keyword(s):  
Pancreatic Islets ◽  
Cell Function ◽  
Culture Media ◽  
Mrna Level ◽  
Interleukin 1Β ◽  
Mrna Levels ◽  
Rat Pancreatic Islets ◽  
Significant Prolongation ◽  
Β Cell Function

In order to elucidate a possible relationship between β-cell function and conversion of proinsulin to insulin, isolated rat pancreatic islets were maintained in tissue culture for 1 week at various glucose concentrations (5.6–56 mM). Studies were also conducted on islets cultured for 48 h with interleukin-1β (IL-1β). By pulse-chase labelling and immunoprecipitation, the relative contents of newly synthesized proinsulin and insulin were determined. ELISA was used to analyse insulin and proinsulin content in medium and within islets. Using real-time PCR, the mRNA levels of proinsulin converting enzymes (PC1 and PC2) were studied. Islets cultured at 56 mM glucose had an increased proportion of newly synthesized proinsulin when compared with islets cultured at 5.6 mM glucose after a 90-min chase periods, however, no difference was observed after culture at 11 and 28 mM glucose. ELISA measurements revealed that culture at increased glucose concentrations as well as islet exposure to IL-1β increased proinsulin accumulation in the culture media. The mRNA expression of PC1 was increased after culture at 11 and 28 mM glucose. Treatment for 48 h with IL-1β increased the proportion of proinsulin both at 45 and 90 min when compared with control islets. These islets also displayed a decreased mRNA level of PC1 as well as PC2. Calculations of the half-time for proinsulin demonstrated a significant prolongation after treatment with IL-1β. We conclude that a sustained functional stimulation by glucose of islets is coupled to a decreased conversion of proinsulin which is also true for islets treated with IL-1β. This may contribute to the elevated levels of proinsulin found both at the onset of type 1 diabetes as well as in type 2 diabetes.

Transplantation of isologous islets of langerhans in diabetic rats. Long-term immunohistochemical results

1977 ◽  
Vol 14 (5-6) ◽  
pp. 199-210 ◽  
Author(s):  
Dietmar Lorenz ◽  
Richard Reding ◽  
Jürgen Petermann ◽  
Hans Lippert ◽  
Volker Worm ◽  
...  
Keyword(s):  
Islets Of Langerhans ◽  
Diabetic Rats

Maintenance of long-term secretory function by microencapsulated islets of Langerhans.

Endocrinology ◽  
1992 ◽  
Vol 130 (2) ◽  
pp. 644-650
Author(s):  
L Lévesque ◽  
P L Brubaker ◽  
A M Sun
Keyword(s):  
Islets Of Langerhans ◽  
Secretory Function
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