Subnormothermic Machine Perfusion Protects Against Rat Liver Preservation Injury: A Comparative Evaluation With Conventional Cold Storage

2007 ◽  
Vol 39 (6) ◽  
pp. 1765-1767 ◽  
Author(s):  
M. Vairetti ◽  
A. Ferrigno ◽  
V. Rizzo ◽  
P. Richelmi ◽  
E. Boncompagni ◽  
...  
Keyword(s):  
Rat Liver ◽  
Cold Storage ◽  
Comparative Evaluation ◽  
Machine Perfusion ◽  
Liver Preservation ◽  
Preservation Injury

Hyperbaric oxygen reduces rat liver preservation injury preserved in a hypotermic machine perfusion

2008 ◽  
Vol 40 (5) ◽  
pp. A14-A15
Author(s):  
F. Giannone ◽  
P. Caraceni ◽  
G. Sgarbi ◽  
A. Baracca ◽  
G. Solaini ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Rat Liver ◽  
Machine Perfusion ◽  
Liver Preservation ◽  
Preservation Injury

Rat Liver Preservation by Hypothermic Oscillating Liver Perfusion Compared to Simple Cold Storage

Cryobiology ◽  
1998 ◽  
Vol 36 (1) ◽  
pp. 61-70 ◽  
Author(s):  
P. Dutkowski ◽  
S. Schönfeld ◽  
B. Odermatt ◽  
T. Heinrich ◽  
T. Junginger
Keyword(s):  
Rat Liver ◽  
Cold Storage ◽  
Liver Perfusion ◽  
Liver Preservation

scholarly journals Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution

2005 ◽  
Vol 11 (5) ◽  
pp. 539-546 ◽  
Author(s):  
Maud Bessems ◽  
Benedict M. Doorschodt ◽  
Arl�ne K. van Vliet ◽  
Thomas M. van Gulik
Keyword(s):  
Rat Liver ◽  
Machine Perfusion ◽  
Preservation Solution ◽  
Liver Preservation

Fatty liver susceptibility to preservation injury using static cold storage versus dynamic machine perfusion

2018 ◽  
Vol 50 (1) ◽  
pp. 8-9
Author(s):  
V. Siciliano ◽  
C. Berardo ◽  
L.G. Di Pasqua ◽  
V. Rizzo ◽  
B. Mannucci ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Cold Storage ◽  
Machine Perfusion ◽  
Preservation Injury

Machine Perfusion at 20°C Prevents Ischemic Injury and Reduces Hypoxia-Inducible Factor-1α Expression During Rat Liver Preservation

2017 ◽  
Vol 22 ◽  
pp. 581-589 ◽  
Author(s):  
Clarissa Berardo ◽  
Laura Giuseppina Di Pasqua ◽  
Veronica Siciliano ◽  
Vittoria Rizzo ◽  
Plinio Richelmi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Hypoxia Inducible Factor ◽  
Ischemic Injury ◽  
Machine Perfusion ◽  
Liver Preservation ◽  
Hypoxia Inducible Factor 1Α

Effect of fructose-1,6-bisphosphate in the cold storage solution after 12 and 36 hours of rat liver preservation

2004 ◽  
Vol 36 (9) ◽  
pp. 2593-2595 ◽  
Author(s):  
R.N. Moresco ◽  
R.C.V. Santos ◽  
J.C.F. Alves Filho ◽  
J.R. De Oliveira
Keyword(s):  
Rat Liver ◽  
Cold Storage ◽  
Liver Preservation ◽  
Fructose 1,6 Bisphosphate ◽  
Storage Solution

Kupffer cell activation in liver preservation: Cold storage vs machine perfusion

2000 ◽  
Vol 32 (1) ◽  
pp. 27-28 ◽  
Author(s):  
J.H Southard ◽  
S Lindell ◽  
M Ametani ◽  
J.-P Richer ◽  
A.W.F Vos
Keyword(s):  
Kupffer Cell ◽  
Cold Storage ◽  
Cell Activation ◽  
Machine Perfusion ◽  
Liver Preservation

scholarly journals Euro-Collins Solution Versus Uw-Solution for Long-Term Liver Preservation in the Isolated Rat-Liver Perfusion Model

HPB Surgery ◽  
1991 ◽  
Vol 4 (4) ◽  
pp. 313-320 ◽  
Author(s):  
René Den Toom ◽  
Marion De Jong ◽  
Eric P. Krenning ◽  
Hans J. Van Der Hoek ◽  
Fiebo J. W. Ten Kate ◽  
...  
Keyword(s):  
Rat Liver ◽  
Cold Storage ◽  
Liver Perfusion ◽  
Ringer Solution ◽  
Metabolic Capacity ◽  
Liver Preservation ◽  
Uw Solution ◽  
Rat Liver Perfusion ◽  
Perfusion Period

To compare UW-solution (UW) and Euro-Collins (EC) for long-term liver preservation we investigated the morphology and metabolic capacity of rat liver after 18 and 42-hours cold-storage in either UW or EC.After harvesting the rat liver was transferred to a perfusion chamber where it was perfused for 10 min with UW or EC at 4°C. Thereafter livers were stored at 4°C in UW or EC for 18 hours (both groups n = 6) or for 42 hours (both groups n = 8). After 18-hr or 42-hr cold-storage a 2-hr warm perfusion (37°C) was started with Krebs-Ringer solution with carbogen to which 125Iodine-triiodothyronine (T3) was added. Control livers (n = 8) were immediately perfused with Krebs-Ringer without cold-storage. The following parameters were assessed: ASAT-levels in the perfusate, T3-metabolites in the bile and the perfusate, the perfusion pressure, the volume of bile secreted and light-microscopical morphology at the end of the warm perfusion period.After cold storage in UW-solution the ASAT-levels in the perfusate were lower than after storage in EC as well as the perfusion pressures. These livers demonstrated a better T3-metabolism and secreted more bile than EC-stored livers. Histological examination showed more tissue damage in the EC-stored livers than in the UW stored livers.We conclude that cold-storage of rat liver in UW-solution resulted in a better morphology and metabolic capacity as compared with EC-solution.

scholarly journals Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

10.3791/52777 ◽  
2015 ◽  
Author(s):  
Bote G. Bruinsma ◽  
James H. Avruch ◽  
Pepijn D. Weeder ◽  
Gautham V. Sridharan ◽  
Basak E. Uygun ◽  
...  
Keyword(s):  
Human Liver ◽  
Machine Perfusion ◽  
Liver Preservation

scholarly journals Characterizing Autophagy in the Cold Ischemic Injury of Small Bowel Grafts: Evidence from Rat Jejunum

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 396
Author(s):  
Ibitamuno Caleb ◽  
Luca Erlitz ◽  
Vivien Telek ◽  
Mónika Vecsernyés ◽  
György Sétáló ◽  
...  
Keyword(s):  
Cold Storage ◽  
Ischemia Reperfusion ◽  
Ischemic Injury ◽  
Degradation Pathway ◽  
Metabolic Adaptation ◽  
Cold Ischemia ◽  
Cold Preservation ◽  
Opposite Trend ◽  
Preservation Injury ◽  
Apoptotic Damage

Cold ischemic injury to the intestine during preservation remains an unresolved issue in transplantation medicine. Autophagy, a cytoplasmic protein degradation pathway, is essential for metabolic adaptation to starvation, hypoxia, and ischemia. It has been implicated in the cold ischemia (CI) of other transplantable organs. This study determines the changes in intestinal autophagy evoked by cold storage and explores the effects of autophagy on ischemic grafts. Cold preservation was simulated by placing the small intestines of Wistar rats in an IGL-1 (Institute George Lopez) solution at 4 °C for varying periods (3, 6, 9, and 12 h). The extent of graft preservation injury (mucosal and cellular injury) and changes in autophagy were measured after each CI time. Subsequently, we determined the differences in apoptosis and preservation injury after activating autophagy with rapamycin or inhibiting it with 3-methyladenine. The results revealed that ischemic injury and autophagy were induced by cold storage. Autophagy peaked at 3 h and subsequently declined. After 12 h of storage, autophagic expression was reduced significantly. Additionally, enhanced intestinal autophagy by rapamycin was associated with less tissue, cellular, and apoptotic damage during and after the 12-h long preservation. After reperfusion, grafts with enhanced autophagy still presented with less injury. Inhibiting autophagy exhibited the opposite trend. These findings demonstrate intestinal autophagy changes in cold preservation. Furthermore, enhanced autophagy was protective against cold ischemia–reperfusion damage of the small bowels.

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