scholarly journals Artificial Skin Model simulating dry and moist in vivo human skin friction and deformation behaviour

2016 ◽  
Vol 97 ◽  
pp. 431-439 ◽  
Author(s):  
M. Nachman ◽  
S.E. Franklin
Keyword(s):  
Skin Friction ◽  
Human Skin ◽  
Deformation Behaviour ◽  
Artificial Skin ◽  
Skin Model

scholarly journals Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Mahmoud Ameri ◽  
Hayley Lewis ◽  
Paul Lehman
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Full Thickness ◽  
Artificial Membrane ◽  
Skin Model ◽  
Hydrophobic Membrane ◽  
Thickness Skin ◽  
In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

scholarly journals The Pathogenesis of Candida Infections in a Human Skin Model: Scanning Electron Microscope Observations

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
A. Raz-Pasteur ◽  
Y. Ullmann ◽  
I. Berdicevsky
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Human Skin ◽  
Fungal Infections ◽  
Ex Vivo ◽  
Skin Model ◽  
High Concentration ◽  
Cutaneous Candidiasis ◽  
Scanning Electron

Cutaneous candidiasis is an opportunistic infection that arises, in most cases, from endogenous, saprophytic candidal blastospores that selectively colonize oral, gastrointestinal, vaginal, and cutaneous epithelium. Candida albicans has been regarded as the most common causative agent in human fungal infections. However, other Candida species have become a significant cause of infection. Scanning electron microscope (SEM) observations were used to analyze the capability of C. albicans, C. tropicalis, and C. parapsilosis to adhere to human skin model, used in this study, which was found to mimic the human skin in vivo. The skin sections were inoculated with low and high concentration of the yeasts and followed for 1 and 5 days; then they were viewed by SEM. The electron microscopy observations revealed that all three yeasts tested adhered to the skin but C. albicans covered the entire skin model to a higher extent than C. tropicalis or C. parapsilosis. Mucin-like material coated the blastoconidia mainly in C. albicans. All Candida species have shown characteristics resembling biofilm formation. The use of human skin sections for ex vivo evaluation of adherence of various yeasts may partially explain the predominance of C. albicans in cutaneous pathogenicity.

scholarly journals Fisetin a 3, 7, 3′, 4′-Tetrahydroxyflavone Inhibits PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models

2019 ◽  
Author(s):  
Jean Christopher Chamcheu ◽  
Stephane Esnault ◽  
Vaqar M. Adhami ◽  
Andrea L. Noll ◽  
Sergette Banang-Mbeumi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Human Skin ◽  
Mononuclear Cells ◽  
Psoriatic Skin ◽  
Epidermal Keratinocytes ◽  
Skin Model ◽  
Human Epidermal Keratinocytes ◽  
Inflammatory Skin ◽  
2D And 3D

Psoriasis is a chronic immune-mediated skin disease that involves interaction of both immune and skin cells, and is characterized by cytokine-driven epidermal hyperplasia, deviant differentiation, inflammation and angiogenesis. Because available treatments for psoriasis have significant limitations, dietary products are potential natural sources of therapeutic molecules, which can rescind molecular defects associated with psoriasis and could be developed for its management. Fisetin (3,7,3′,4′- tetrahydroxyflavone), a phytochemical naturally found in pigmented fruits and vegetables has demonstrated pro-apoptotic and antioxidant effects in several malignancies. This study utilized biochemical, cellular, pharmacological and tissue-engineering tools to characterize the effects of fisetin on normal human epidermal keratinocytes (NHEKs), peripheral blood mononuclear cells (PBMC) and CD4+ T lymphocytes in 2D and 3D psoriasis-like disease models. Fisetin treatment of NHEKs dose and time-dependently induced differentiation and inhibited interleukin-22-induced proliferation, as well as activation of the PI3K/Akt/mTOR pathway. Fisetin treatment of TNF-α-stimulated NHEKs significantly inhibited the activation of p38 and JNK, but had no effect on ERK1/2. In addition, fisetin treatment significantly decreased the secretion of Th1/Th-17 pro-inflammatory cytokines, particularly IFNγ and IL-17A by 12-O- tetradecanolylphorbol 13-acetate (TPA)-stimulated NHEKs and anti-CD3/CD28-activated human PBMCs. Furthermore, we established the in-vivo relevance of fisetin functions, using a 3D full-thickness human skin model of psoriasis (FTRHSP) that closely mimics in-vivo human psoriatic skin-lesions. Herein, fisetin significantly ameliorated psoriasis-like disease features, and decreased the production of IL-17 by CD4+ T lymphocytes co-cultured with FTRHSP. Collectively, our data identify pro-differentiative, anti-proliferative and anti-inflammatory effects of fisetin, via modulation of PI3K-Akt-mTOR and p38/JNK pathways and the production of cytokines in 2D and 3D human skin model of psoriasis. These results suggest that fisetin has a great potential to be developed as an effective and inexpensive agent for the treatment of psoriasis and other related inflammatory skin disorders.

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