Development and evaluation of a species-specific PCR assay for the detection of Brucella ovis infection in rams

2010 ◽  
Vol 145 (1-2) ◽  
pp. 158-164 ◽  
Author(s):  
Mariana N. Xavier ◽  
Teane M.A. Silva ◽  
Érica A. Costa ◽  
Tatiane A. Paixão ◽  
Valéria S. Moustacas ◽  
...  
Keyword(s):  
Pcr Assay ◽  
Brucella Ovis ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

Species-specific PCR assay for L. infantum/L. donovani discrimination

Acta Tropica ◽  
2006 ◽  
Vol 100 (3) ◽  
pp. 241-245 ◽  
Author(s):  
Mallorie Hide ◽  
Anne-Laure Bañuls
Keyword(s):  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

scholarly journals Simultaneous Detection and Identification of Common Cell Culture Contaminant and Pathogenic Mollicutes Strains by Reverse Line Blot Hybridization

2004 ◽  
Vol 70 (3) ◽  
pp. 1483-1486 ◽  
Author(s):  
Hui Wang ◽  
Fanrong Kong ◽  
Peter Jelfs ◽  
Gregory James ◽  
Gwendolyn L. Gilbert
Keyword(s):  
Cell Culture ◽  
16S Rrna ◽  
Cell Line ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Pcr Assay ◽  
Intergenic Spacer Region ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

ABSTRACT We have developed a reverse line blot (RLB) hybridization assay to detect and identify the commonest mollicutes causing cell line contamination (Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma orale, and Acholeplasma laidlawii) and human infection (Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma parvum, and Ureaplasma urealyticum). We developed a nested PCR assay with “universal” primers targeting the mollicute 16S-23S rRNA intergenic spacer region. Amplified biotin-labeled PCR products were hybridized to membrane-bound species-specific oligonucleotide probes. The assay correctly identified reference strains of 10 mollicute species. Cell cultures submitted for detection of mollicute contamination, clinical specimens, and clinical isolates were initially tested by PCR assay targeting a presumed mollicute-specific sequence of the 16S rRNA gene. Any that were positive were assessed by the RLB assay, with species-specific PCR assay as the reference method. Initially, 100 clinical and 88 of 92 cell culture specimens gave concordant results, including 18 in which two or more mollicute species were detected by both methods. PCR and sequencing of the 16S-23S rRNA intergenic spacer region and subsequent retesting by species-specific PCR assay of the four cell culture specimens for which results were initially discrepant confirmed the original RLB results. Sequencing of amplicons from 12 cell culture specimens that were positive in the 16S rRNA PCR assay but negative by both the RLB and species-specific PCR assays failed to identify any mollicute species. The RLB hybridization assay is sensitive and specific and able to rapidly detect and identify mollicute species from clinical and cell line specimens.

Rapid and Reliable Identification of Staphylococcus equorum by a Species-Specific PCR Assay Targeting the sodA Gene

2004 ◽  
Vol 27 (6) ◽  
pp. 696-702 ◽  
Author(s):  
Giuseppe Blaiotta ◽  
Danilo Ercolini ◽  
Gianluigi Mauriello ◽  
Giovanni Salzano ◽  
Francesco Villani
Keyword(s):  
Pcr Assay ◽  
Reliable Identification ◽  
Specific Pcr ◽  
Staphylococcus Equorum ◽  
Specific Pcr Assay ◽  
Species Specific
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