Host–pathogen gene expression profiles during infection of primary bovine mammary epithelial cells with Escherichia coli strains associated with acute or persistent bovine mastitis

AbstractIn this research communication, a cell model with elevated β-CASEIN synthesis was established by stimulating bovine mammary epithelial cells with 0.6 mM methionine, and the genome-wide gene expression profiles of methionine-stimulated cells and untreated cells were investigated by RNA sequencing. A total of 458 differentially expressed genes (DEGs; 219 upregulated and 239 downregulated) were identified between the two groups. Gene Ontology (GO) analysis showed that the two highest-ranked GO terms in ‘molecular function’ category were ‘binding’ and ‘catalytic activity’, suggesting that milk protein synthesis in methionine-stimulated cells requires induction of gene expression to increase metabolic activity. Kyoto Encyclopedia of Genes and Genomes analysis revealed that within the ‘environmental information processing’ category, the subcategory that is most highly enriched for DEGs was ‘signal transduction’. cGMP-PKG, Rap1, calcium, cAMP, PI3K-AKT, MAPK, and JAK-STAT are the pathways with the highest number of DEGs, suggesting that these signaling pathways have potential roles in mediating methionine-induced milk protein synthesis in bovine mammary epithelial cells. This study provides valuable insights into the physiological and metabolic adaptations in cells stimulated with methionine. Understanding the regulation of this transition is essential for effective intervention in the lactation process.

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ESBL-Producing Escherichia coli from Bovine Mastitis Induced Apoptosis of Bovine Mammary Epithelial Cells Via Alteration of ROS/MMP/bax/bcl-2 Signaling Pathway

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2020.038 ◽

2020 ◽

Author(s):

Yuxiang Shi

Keyword(s):

Escherichia Coli ◽

Epithelial Cells ◽

Signaling Pathway ◽

Mammary Epithelial Cells ◽

Bovine Mastitis ◽

Mammary Epithelial ◽

Bovine Mammary Epithelial Cells ◽

Induced Apoptosis

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Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

Czech Journal of Animal Science ◽

10.17221/6347-cjas ◽

2012 ◽

Vol 57 (No. 10) ◽

pp. 469-480 ◽

Cited By ~ 15

Author(s):

T. Sigl ◽

H.H.D. Meyer ◽

S. Wiedemann

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Milk Protein ◽

Protein Gene ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Milk Protein Gene ◽

Bovine Mammary Epithelial Cells ◽

Milk Protein Genes ◽

Milk Protein Gene Expression

The objective of the present study was to refine a previously developed method to isolate primary bovine mammary epithelial cells (pBMEC) from fresh milk. Using this method, it was tested whether the number of pBMEC and the relation of recovered pBMEC to total somatic cell count vary within the individual lactation stages. Furthermore, the expression levels of the milk protein genes during the first twenty weeks of lactation were determined by quantitative PCR method. A total number of 152 morning milk samples were obtained from twenty-four Holstein-Friesian cows during the first 20 weeks of lactation (day 8, 15, 26, 43, 57, 113, and 141 postpartum). Numbers of extracted pBMEC were consistent at all time-points (1.1 ± 0.06 to 1.4 ± 0.03 ×103/ml) and an average value of RNA integrity number (RIN) was 6.3 ± 0.3. Percentage of pBMEC in relation to total milk cells (2.0 ± 0.2 to 6.7 ± 1.0%) correlated with milk yield. Expression patterns of the casein genes alpha (α)S1, (α)S2, beta (β), and kappa (κ) (CSN1S1, CSN1S2, CSN2, CSN3, respectively) and the whey protein genes α-lactalbumin (LALBA) and progestagen-associated endometrial protein (PAEP; known as β-lactoglobulin) were shown to be comparable, i.e. transcripts of all six milk protein genes were found to peak during the first two weeks of lactation and to decline continuously towards mid lactation. However, mRNA levels were different among genes with CSN3 showing the highest and LALBA the lowest abundance. We hypothesized that milk protein gene expression has a pivotal effect on milk protein composition with no influence on milk protein concentration. This paper is the first to describe milk protein gene expression during lactation in pBMEC collected in milk. Future studies will be needed to understand molecular mechanisms in pBMEC including regulation of expression and translation throughout lactation.

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