Involvement of tannins and flavonoids in the in vitro effects of Newbouldia laevis and Zanthoxylum zanthoxyloïdes extracts on the exsheathment of third-stage infective larvae of gastrointestinal nematodes

2011 ◽  
Vol 180 (3-4) ◽  
pp. 292-297 ◽  
Author(s):  
E.V.B. Azando ◽  
M.S. Hounzangbé–Adoté ◽  
P.A. Olounladé ◽  
S. Brunet ◽  
N. Fabre ◽  
...  
Keyword(s):  
Gastrointestinal Nematodes ◽  
Infective Larvae ◽  
Third Stage ◽  
Zanthoxylum Zanthoxyloides ◽  
In Vitro Effects

scholarly journals Effect of Five Mediterranean Shrubs Extracts on Larval Exsheathment of Haemonchus contortus

2021 ◽  
Author(s):  
A. Aïssa ◽  
F. Manolaraki ◽  
H. Ben Salem ◽  
H. Hoste ◽  
K. Kraiem
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Good Alternative ◽  
Gastrointestinal Nematodes ◽  
Shrub Species ◽  
Medicago Arborea ◽  
Mediterranean Shrubs ◽  
Third Stage

Background: Mediterranean shrub species cover more than 70% of the total area in Tunisia and in summer when the herbaceous species have wilted, they constitute feeding resource for livestock. The use of tanniniferous shrubs seems to be a good alternative to control gastrointestinal nematodes infections in small ruminants. This study evaluated the in vitro anthelmintic (AH) effect of Ceratonia siliqua (C. siliqua), Periploca angustifolia Labill. (P. angustifolia) and Medicago arborea (M. arborea) against Haemonchus contortus third stage larvae (L3). Methods: The larval exsheathment assay (LEA) was used to determine the proportions (%) of exsheathment of five acetonic extracts at different concentrations (1200, 600, 300, 150 μg/ml). To confirm the role of tannins in the AH effects of extracts, polyvinyl polypyrolidone (PVPP) was used as deactivating chemical tannins. Result: The highest % L3 exsheathed was recorded for M. arborea (55.01%) and the lowest value was founded for C. siliqua and P. angustifolia leaves (16.26%). Our results were concentration-dependent (P less than 0.001). The % of exsheathment increased as the time of incubation increased (P less than 0.001). P. angustifolia pods recorded the lowest EC50 value (P less than 0.05). After PVPP addition, all the acetonic extracts showed a restoration of L3 exsheathment values similar to control values (P less than 0.001).

scholarly journals In vitro and in vivo anthelmintic activity of extracts from Artemisia parviflora and A. sieversiana

2017 ◽  
Vol 54 (3) ◽  
pp. 218-224 ◽  
Author(s):  
S. Irum ◽  
H. Ahmed ◽  
B. Mirza ◽  
K. Donskow-Łysoniewska ◽  
A. Muhammad ◽  
...  
Keyword(s):  
Adult Worm ◽  
Plant Extracts ◽  
Small Ruminants ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Economic Losses ◽  
Egg Hatching ◽  
Infective Larvae

SummaryIn the northern areas of Pakistan, the use of Artemisia based therapeutics is a common practice. Plants of genus Artemisia are known to possess anthelmintic and therapeutic effect. Infections caused by gastrointestinal nematodes are major threat to livestock industry across the world resulting in loss of production and indirect economic losses due to high cost of anthelmintic drugs. Present study was carried out to evaluate in vitro and in vivo effect of Artemisia sieversiana and Artemisia parviflora on Haemonchus contortus, a parasitic nematode of small ruminants. Methanolic plant extract was tested against three different developmental stages using an egg hatch assay, infective larvae and adult worm motility assay. Different concentrations were used for the bioassays and post exposure mortality was recorded after 8 hr for adult worms and infective larvae, while egg inhibition percentage was observed after 27 hr. A highly significant ability to inhibit the egg hatching (100 %) was recorded for both plant extracts while, the highest activity for adult worm assay and larvicidal assay was 90 % for A. sieversiana. The highest activity for adult motility and larvicidal assay for A. parviflora was 89 % and 86.6 % respectively. For in vivo trials maximum parentage reduction was 77.0 % for A. sieversiana and 73.6 % for A. parviflora. It is concluded that selected plant extracts were effective in reducing worm burden in animals.

scholarly journals In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus

2020 ◽  
Vol 8 (1) ◽  
pp. 93-99
Author(s):  
Esaïe Tchetan ◽  
Erick V. B. Azando ◽  
Pascal A. Olounladé ◽  
Géorcelin G. Alowanou ◽  
Sylvie M. Hounzangbé-Adoté
Keyword(s):  
Bridelia Ferruginea ◽  
Infective Larvae ◽  
In Vitro Effects ◽  
Mitragyna Inermis

In vitro trapping capability of Arthrobotrys spp. on infective larvae of Haemonchus contortus and Nacobbus aberrans

1994 ◽  
Vol 68 (3) ◽  
pp. 223-229 ◽  
Author(s):  
P. Mendoza-de Gives ◽  
E. Zavaleta-Mejia ◽  
D. Herrera-Rodriguez ◽  
H. Quiróz-Romero
Keyword(s):  
Microscopic Examination ◽  
Haemonchus Contortus ◽  
Three Dimensional ◽  
Arthrobotrys Oligospora ◽  
Second Stage ◽  
Infective Larvae ◽  
Third Stage ◽  
Nacobbus Aberrans

AbstractThe trapping capability of Arthrobotrys oligospora and A. conoides (Hyphomycetales) against third stage larvae (L3) of Haemonchus contortus (Trichostrongylidae) was evaluated in an in vitro trial. Arthrobotrys oligospora showed a 35.87% and 25.71% trapping effectiveness against H. contortus infective larvae at 18 and 25°C, respectively; whereas the trapping capability of A. conoides was 92.17% and 90.40% at the same temperatures, respectively. Microscopic examination demonstrated that A. conoides spontaneously developed a large quantity of three-dimensional loops before the nematodes were added. Neither of the two species studied developed three-dimensional adhesive loops at 30°C, consequently no trapped nematode was observed. In a second trial, the trapping capability of A. conoides against H. contortus (L3) and second stage larvae (J2) of Nacobbus aberrans (Pratylenchidae), was evaluated at 25°C. The trapping capability shown by A. conoides was higher than 90% for both kinds of nematode. The possible use of A. conoides to control ovine haemonchosis is discussed.

scholarly journals IN VITRO EFFECTS OF SOME HOUSEHOLD CHEMICALS ON INFECTIVE LARVAE OF HAEMONCHUS CONTORTUS OF GOAT

2012 ◽  
Vol 3 (1) ◽  
pp. 55-58 ◽  
Author(s):  
M Shahiduzzaman ◽  
MK Islam ◽  
M Rahman ◽  
MMH Mondal
Keyword(s):  
Sodium Chloride ◽  
Boric Acid ◽  
Potassium Permanganate ◽  
Haemonchus Contortus ◽  
Larvicidal Effect ◽  
Infective Larvae ◽  
Bleaching Powder ◽  
In Vitro Effects ◽  
Household Chemicals

In vitro effects of some household chemicals on infective larvae (L3) of Haemonchus contortus of goat were screened during the period from January 2002 to April 2003. Solutions of 0.5, 1, 5, 10 and 20 mg / ml were screened for in vitro effects on infective larvae (L3) of Haemonchus contortus. The percent non-motile L3 was increased when immersed in increasing solutions of the household chemicals (p < 0.01) except sodium chloride. Of 10 household chemicals, potassium permanganate showed 100% in vitro larvicidal effect at 1 mg / ml concentration. Boric acid showed the second highest larvicidal effect (78.95%) followed by alum (71.86%), bleaching powder (71.43%) and borax (68.97%) at their highest concentration of 20 mg / ml.

Studies with Brugia pahangi 12. The activity of levamisole

1976 ◽  
Vol 50 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Rosemary Rogers ◽  
D. A. Denham
Keyword(s):  
Aedes Aegypti ◽  
Developmental Stages ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Vector Mosquito ◽  
Third Stage ◽  
Dose Levels

AbstractThe effects of levamisole on adults, third stage infective larvae, and microfilariae of Brugia pahangi were studied in in vitro culture and in vivo against developing stages in the vector mosquito and in infected cats. In vitro the drug was effective only at dose levels much higher than can be tolerated by mammals. It was active against the developmental stages of the worm in the vector Aedes aegypti.The drug was strongly microfilaricidal in cats but less effective against adult worms.

scholarly journals In vitro chemotactic responses of Brugia pahangi infective larvae to sodium ions

2011 ◽  
Vol 86 (4) ◽  
pp. 406-409 ◽  
Author(s):  
Y. Mitsui ◽  
M. Miura ◽  
D.A. Bome ◽  
Y. Aoki
Keyword(s):  
Deionized Water ◽  
Sodium Ions ◽  
Brugia Pahangi ◽  
Low Concentration ◽  
Infective Larvae ◽  
Significant Tendency ◽  
Third Stage

AbstractIn vitro chemotactic responses of infective third-stage larvae (L3) of Brugia pahangi to NaCl, Na2HPO4, KCl, K2HPO4, MgCl2 and CaCl2 were assessed. Compared to deionized water as a control, 200 mm NaCl and 100 mm Na2HPO4 significantly attracted L3 (P < 0.01 and P < 0.01), whereas L3 were likely to avoid 200 mm KCl and 100 mm K2HPO4 (P < 0.05 and P < 0.05). L3 showed no significant tendency to avoid or to be attracted to 200 mm CaCl2 and 200 mm MgCl2. Furthermore, NaCl exhibited a significant chemoattractant activity for L3 at a low concentration of 100 mm.

scholarly journals Effects of selected Palestinian plants on the in vitro exsheathment of the third stage larvae of gastrointestinal nematodes

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Rana Majed Jamous ◽  
Mohammed Saleem Ali-Shtayeh ◽  
Salam Yousef Abu-Zaitoun ◽  
Alex Markovics ◽  
Hassan Azaizeh
Keyword(s):  
Gastrointestinal Nematodes ◽  
The Third ◽  
Third Stage

In vitro anthelmintic activity of active compounds of the fringed rue Ruta chalepensis against dairy ewe gastrointestinal nematodes

2016 ◽  
Vol 91 (4) ◽  
pp. 447-453 ◽  
Author(s):  
E. Ortu ◽  
G. Sanna ◽  
A. Scala ◽  
G. Pulina ◽  
P. Caboni ◽  
...  
Keyword(s):  
Essential Oil ◽  
Small Ruminant ◽  
Methanol Extract ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Economic Losses ◽  
Ruta Chalepensis ◽  
Alternative Ingredients ◽  
Third Stage

AbstractInfections by gastrointestinal nematodes negatively affect small ruminant health and at the same time cause substantial economic losses worldwide. Because resistance to conventional anthelmintic compounds is growing, target studies evaluating the effectiveness of alternative ingredients of botanical origin on gastrointestinal nematodes are needed. In this study, we evaluated the in vitro anthelmintic activity of Ruta chalepensis L. extracts on the third-stage larvae of sheep gastrointestinal nematodes. A methanol extract showed the highest anthelmintic activity, with an EC50 = 0.10 ± 0.06 mg/ml after 96 h, while the essential oil had an EC50 = 1.45 ± 1.22 mg/ml after 48 h. Moreover, three secondary metabolites of the essential oil, i.e. 2-decanone, 2-nonanone and 2-undecanone, showed EC50 values of 0.07 ± 0.06, 0.25 ± 0.29 and 0.88 ± 0.73 mg/ml at 24 h, respectively. The present study indicated that the R. chalepensis methanol extract, the essential oil and its metabolites 2-decanone, 2-nonanone and 2-undecanone showed promising anthelmintic activity on gastrointestinal nematodes.

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