Critical residues for proteolysis activity of maize chlorotic dwarf virus (MCDV) 3C-like protease and comparison of activity of orthologous waikavirus proteases

Virology ◽  
2021 ◽  
Author(s):  
Lucy R. Stewart ◽  
Matthew Willman ◽  
DeeMarie Marty ◽  
Anna (Emmick) Cole ◽  
Kristen Willie
Keyword(s):  
Dwarf Virus ◽  
Critical Residues ◽  
Maize Chlorotic Dwarf Virus

Maize Chlorotic Dwarf Virus Group

2018 ◽  
pp. 111-116
Author(s):  
R. I. B. Francki ◽  
Robert G. Milne ◽  
T. Hatta
Keyword(s):  
Dwarf Virus ◽  
Virus Group ◽  
Maize Chlorotic Dwarf Virus

scholarly journals Genomic of the severe isolate of Maize chlorotic dwarf virus

2010 ◽  
Vol 40 (No. 4) ◽  
pp. 113-119 ◽  
Author(s):  
R. Chaouch ◽  
G. Redinbaugh M ◽  
M. Marrakchi ◽  
S.A. Hogenhout
Keyword(s):  
Type Strain ◽  
Dwarf Virus ◽  
Cdna Clones ◽  
Large Open Reading Frame ◽  
Reading Frame ◽  
Protein Motifs ◽  
Complete Sequences ◽  
The Difference ◽  
Maize Chlorotic Dwarf Virus ◽  
Severe Isolate

The complete sequences of the severe isolates of Maize chlorotic dwarf virus (MCDV-S) were obtained from cDNA clones of the viral RNA. The genome is 11 785 nucleotides (nt) in length and contains one large open reading frame between nt 455 and 10 825 that encodes a polyprotein of 3458 amino acids. Computer analysis revealed several conserved protein motifs similar to other plant picorna-like viruses. Comparison of the genome sequence of MCDV-S with that of the type strain of MCDV showed high similarities despite the difference in symptoms.  

scholarly journals Identification of a maize chlorotic dwarf virus silencing suppressor protein

Virology ◽  
2017 ◽  
Vol 504 ◽  
pp. 88-95 ◽  
Author(s):  
Lucy R. Stewart ◽  
Sridhar Jarugula ◽  
Yujing Zhao ◽  
Feng Qu ◽  
DeeMarie Marty
Keyword(s):  
Dwarf Virus ◽  
Silencing Suppressor ◽  
Maize Chlorotic Dwarf Virus

scholarly journals Co-infection and Disease Severity of Ohio Maize dwarf mosaic virus and Maize chlorotic dwarf virus Strains

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1661-1665 ◽  
Author(s):  
Katia Morales ◽  
Jose Luis Zambrano ◽  
Lucy R. Stewart
Keyword(s):  
Mosaic Virus ◽  
Sweet Corn ◽  
Growth Parameters ◽  
Quantitative Polymerase Chain Reaction ◽  
The United States ◽  
Dwarf Virus ◽  
Maize Dwarf Mosaic Virus ◽  
Synergistic Enhancement ◽  
The Impact ◽  
Maize Chlorotic Dwarf Virus

Two major maize viruses have been reported in the United States: Maize dwarf mosaic virus (MDMV) and Maize chlorotic dwarf virus (MCDV). These viruses co-occur in regions where maize is grown, such that co-infections are likely. Co-infection of different strains of MCDV is also observed, and a synergistic enhancement of symptoms in co-infected plants was previously reported. Here, we examined the impact of co-infections of two strains of MCDV (MCDV-S and MCDV-M1, severe and mild, respectively), and co-infections of MCDV and MDMV in the sweet corn hybrid ‘Spirit’ in greenhouse experiments. Quantitative plant growth and development parameters were measured and virus accumulation was measured by reverse-transcriptase quantitative polymerase chain reaction. Virus symptoms were enhanced and plants showed no recovery over time in co-infections of MDMV-OH and MCDV-S but virus titers and quantitative growth parameters did not indicate synergy in co-infected plants. MCDV-M1 co-infections with either MDMV-OH or MCDV-S did not show symptom enhancement or evidence of synergism.

Cytopathology and ultrastructure of mild and severe strains of maize chlorotic dwarf virus in maize and johnsongrass

1993 ◽  
Vol 71 (5) ◽  
pp. 718-724 ◽  
Author(s):  
E. D. Ammar ◽  
R. E. Gingery ◽  
L. R. Nault
Keyword(s):  
Electron Microscopy ◽  
Uranyl Acetate ◽  
Dwarf Virus ◽  
Mesophyll Cells ◽  
White Stripe ◽  
Cytoplasmic Inclusions ◽  
Maize Leaves ◽  
Maize Chlorotic Dwarf Virus ◽  
In Cells

In maize leaves experimentally infected with various isolates or strains of maize chlorotic dwarf virus, including a newly characterized strain (M1), and in naturally infected johnsongrass, only two types of cytoplasmic inclusions were consistently observed: (i) quasi-spherical electron-dense granular inclusions, and (ii) curved or straight bundles of fibrous inclusions. Both types were detected by light and (or) electron microscopy in vascular parenchyma and phloem cells, and less frequently in bundle-sheath and adjacent mesophyll cells. The dense granular inclusions usually contained numerous isometric virus-like particles, some of which may have been released into the surrounding cytoplasm. However, a high proportion of these inclusions in cells infected with the mild type strain and a type-like isolate (M8) were either devoid of or contained very few viruslike particles. In maize leaves infected with the white stripe (WS) isolate, the chloroplasts were markedly deformed; in leaves of stunted plants doubly infected with M8 and the serologically distinct M1 strain, some phloem cells appeared degenerated. Electron microscopy of preparations of purified M1 stained with uranyl acetate revealed both stain-impenetrable full particles and stain-penetrable empty or partially empty particles. Both full and apparently empty particles were also found in cells of maize leaves infected with M1, whereas with other strains and isolates, mainly full particles were found both in situ and in vitro. Key words: maize chlorotic dwarf virus, cytopathology, ultrastructure, maize, johnsongrass.

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