A CRISPR-Cas9 Generated MDCK Cell Line Expressing Human MDR1 Without Endogenous Canine MDR1 ( cABCB1 ): An Improved Tool for Drug Efflux Studies

2017 ◽  
Vol 106 (9) ◽  
pp. 2909-2913 ◽  
Author(s):  
Maria Karlgren ◽  
Ivailo Simoff ◽  
Maria Backlund ◽  
Christine Wegler ◽  
Markus Keiser ◽  
...  
Keyword(s):  
Cell Line ◽  
Mdck Cell ◽  
Drug Efflux ◽  
Mdck Cell Line ◽  
Human Mdr1

Establishment and characterization of an MDCK cell line stably-transfected with chicken Abcb1 encoding P-glycoprotein

2016 ◽  
Vol 106 ◽  
pp. 37-44 ◽  
Author(s):  
Yong Sun ◽  
Tingting Guo ◽  
Dawei Guo ◽  
Li Guo ◽  
Li Chen ◽  
...  
Keyword(s):  
Cell Line ◽  
Mdck Cell ◽  
Mdck Cell Line ◽  
P Glycoprotein

Comparison of influenza virus yields and apoptosis-induction in an adherent and a suspension MDCK cell line

Vaccine ◽  
2013 ◽  
Vol 31 (48) ◽  
pp. 5693-5699 ◽  
Author(s):  
B. Peschel ◽  
S. Frentzel ◽  
T. Laske ◽  
Y. Genzel ◽  
U. Reichl
Keyword(s):  
Influenza Virus ◽  
Cell Line ◽  
Mdck Cell ◽  
Apoptosis Induction ◽  
Mdck Cell Line

Optimization of trypsins for influenza A/H1N1 virus replication in MDCK SI-6 cells, a novel MDCK cell line

2018 ◽  
Vol 252 ◽  
pp. 94-99
Author(s):  
Viska I. Iskandar ◽  
Yutaka Sasaki ◽  
Naoto Yoshino ◽  
Raden Z.R. Abubakar ◽  
Shigehiro Sato ◽  
...  
Keyword(s):  
Cell Line ◽  
Virus Replication ◽  
Mdck Cell ◽  
Influenza A ◽  
H1n1 Virus ◽  
Mdck Cell Line ◽  
Influenza A H1n1

scholarly journals Heterogeneity of the MDCK Cell Line and Its Applicability for Influenza Virus Research

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75014 ◽  
Author(s):  
Vladimir Y. Lugovtsev ◽  
Darya Melnyk ◽  
Jerry P. Weir
Keyword(s):  
Influenza Virus ◽  
Cell Line ◽  
Mdck Cell ◽  
Mdck Cell Line ◽  
Virus Research

Assessment of aspects of the toxicity of Clostridium perfringens E-toxin using the MDCK cell line

1996 ◽  
Vol 15 (11) ◽  
pp. 904-908 ◽  
Author(s):  
Christopher D Lindsay
Keyword(s):  
Cell Line ◽  
Clostridium Perfringens ◽  
Mechanism Of Action ◽  
Sodium Azide ◽  
Mdck Cell ◽  
Mdck Cells ◽  
Neutral Red ◽  
Temperature Sensitive ◽  
Mdck Cell Line ◽  
Neutral Red Assay

1 The epithelial Madin Darby Canine Kidney (MDCK) cell line was used to study the toxicity of ∈-toxin from Clostridium perfringens. The epithelial MDCK cell line is known to be sensitive to ∈-toxin of Clostridium perfringens and to investigate its mechanism of action, the neutral red assay has been used to determine the viability of cultures of this cell line. 2 Comparison of the LC 50s obtained at 34°C and 0°C showed that the lethality of ∈-toxin was reduced by 18- fold at the lower temperature. The effect of tempera ture on ∈-toxin lethality is unlikely to be due to reductions in membrane fluidity for the addition of Ca2+ or Mg2+ (2 mM) to buffer containing toxin was without effect. Varying the pH of the toxin-containing buffer from 6.9 to 8.7 did not increase the lethality of the toxin, though the most acidic pH used (5.8) was found to potentiate its action on MDCK cells. 3 The effect of inhibiting endocytosis on the lethality ofe toxin was also investigated by incubating cultures of MDCK cells with and without sodium azide over a range of concentrations of toxin. The co-administra tion of sodium azide did not reduce the toxicity of ∈ toxin, suggesting that energy-dependent uptake pro cesses such as endocytosis were unlikely to be involved in its mechanism of action. The results are, however, consistent with known receptor-based me chanisms of uptake and with other mechanisms of internalisation across the plasma membrane. ∈-toxin thus interacts with cell surfaces by a temperature sensitive mechanism potentiated by low pH.

Production of Live Influenza Vaccine in MDCK Cell Line

2005 ◽  
pp. 783-785
Author(s):  
N. Mazurkova ◽  
E. Nechaeva ◽  
T. Ryabicheva ◽  
N. Varaksin ◽  
T. Sen'Kina ◽  
...  
Keyword(s):  
Influenza Vaccine ◽  
Cell Line ◽  
Mdck Cell ◽  
Mdck Cell Line

MDCK cell line with inducible allele B NS1 expression propagates delNS1 influenza virus to high titres

Vaccine ◽  
2011 ◽  
Vol 29 (40) ◽  
pp. 6976-6985 ◽  
Author(s):  
R. van Wielink ◽  
M.M. Harmsen ◽  
D.E. Martens ◽  
B.P.H. Peeters ◽  
R.H. Wijffels ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Cell Line ◽  
Mdck Cell ◽  
Mdck Cell Line ◽  
Ns1 Expression
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