The rapid separation and isolation of mono- and oligo-nucleotides by high speed liquid chromatography: An ion exchange, reversed-phase system

1974 ◽  
Vol 58 (3) ◽  
pp. 605-612 ◽  
Author(s):  
R.A. Holton ◽  
D.M. Spatz ◽  
E.E. van Tamelen ◽  
W. Wierenga
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
High Speed ◽  
Reversed Phase ◽  
Phase System ◽  
Rapid Separation

Urinary free catecholamines determined by liquid chromatography--fluorometry.

1981 ◽  
Vol 27 (12) ◽  
pp. 2060-2063 ◽  
Author(s):  
G M Anderson ◽  
J G Young ◽  
P I Jatlow ◽  
D J Cohen
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Internal Standard ◽  
Reversed Phase ◽  
Ion Pair ◽  
Native Fluorescence ◽  
Exchange Column ◽  
Ion Pair Chromatography ◽  
Ion Exchange Column

Abstract Free norepinephrine, epinephrine, and dopamine are determined in urine after purification on a small ion-exchange column and concentration on alumina. The catecholamines are separated by reversed-phase ion-pair chromatography and are quantitated by measuring their native fluorescence (lambda ex 285 nm, lambda em 305 nm). The internal standard is dihydroxybenzylamine. Within-day CVs for the compounds ranged from 1.6 to 5.6%; between-day CVs were 6.6 to 9.8%.

Sign in / Sign up

Export Citation Format

Share Document