P-084 P1/SSS embryo culture medium yields a higher pregnancy rate than HTF/plasmanate in IVF/ET

1997 ◽  
Vol 68 ◽  
pp. S132 ◽  
Author(s):  
C.B Barrett ◽  
A.S Penzias ◽  
R.D Powers
Keyword(s):  
Pregnancy Rate ◽  
Embryo Culture ◽  
Culture Medium ◽  
Embryo Culture Medium ◽  
Ivf Et

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

251 FACTORS INFLUENCING THE SUCCESS OF EQUINE INTRACYTOPLASMIC SPERM INJECTION IN A CLINICAL PROGRAM

2016 ◽  
Vol 28 (2) ◽  
pp. 258
Author(s):  
K. Hinrichs ◽  
Y. H. Choi
Keyword(s):  
Pregnancy Rate ◽  
Density Gradient ◽  
Embryo Culture ◽  
Intracytoplasmic Sperm Injection ◽  
Culture Medium ◽  
Ongoing Pregnancy ◽  
Ongoing Pregnancy Rate ◽  
Immature Oocytes ◽  
Embryo Culture Medium ◽  
Blastocyst Rate

Intracytoplasmic sperm injection (ICSI) is currently being used clinically in horses, but little information is available on factors affecting its success. We have conducted research trials and evaluated data from our clinical ICSI caseload, currently over 450 cases per year, to provide information in this area. In the following summaries, blastocyst data are reported per injected oocyte; clinical data are from 2013 and 2014. Clinically, when immature follicles were aspirated, the number of follicles aspirated per mare decreased significantly with mare age, from 16.2 at age 12–15 to 8.9 at age 24–25; however, maturation and blastocyst rates of recovered oocytes did not differ significantly. Immature oocytes shipped to the laboratory by referring veterinarians yielded a significantly higher blastocyst rate than that for immature oocytes aspirated at the laboratory (27 v. 21%, respectively). Shipped oocytes recovered from stimulated preovulatory follicles yielded a higher blastocyst rate per oocyte than did shipped immature oocytes (39 v. 27%, respectively), but provided fewer mature oocytes per aspiration (1.0 v. 4.5 for immature). From research data, administration of gentamicin and ampicillin to mares before immature oocyte aspiration did not affect blastocyst rate. Holding the aspirate for ~1.5 h at ambient temperature (26 to 33°C) was associated with a blastocyst rate of 32%; however, holding for 2 h at 32°C yielded only 16% blastocysts v. 23% for control. Blastocysts (18%) were obtained from oocytes recovered in the nonbreeding season (December and January). Holding oocytes at room temperature overnight before maturation did not affect blastocyst rate (25 to 34%), nor did inclusion of zinc in the maturation medium (18 to 31%). Diluting and refreezing semen to increase doses available for ICSI did not affect blastocyst rate (23 to 27%); blastocysts (13%) were also obtained after injection of nonmotile sperm. Significant differences in cleavage and blastocyst rates were identified among stallions. For one stallion, use of density gradient plus swim-up before ICSI increased cleavage (49 v. 18%) and blastocyst rates (11 v. 0%) compared to density gradient alone. Blastocyst production was not affected by the amount of glucose added to a human embryo culture medium (0 or 5 mM added glucose on Days 0–5, then 10 or 20 mM added glucose; 31 to 46% blastocysts). Replacement of 10% FBS in embryo culture medium with a mixture of FBS, human serum replacement, and equine follicular fluid lowered blastocyst rate (15 v. 37% for FBS alone). Clinically, embryos were vitrified or were shipped to embryo transfer centers for transfer. There was no significant difference in ongoing pregnancy rate for embryos from shipped immature v. preovulatory oocytes (54 and 69%). For immature oocytes, embryos developing to blastocyst on Day 10 or 11 had a lower ongoing pregnancy rate after transfer (40 and 0%) than did those developing on Days 7 to 9 (51 to 75%). This work was supported by the Link Equine Research Endowment Fund, Texas A&M University, and by the Clinical Equine ICSI Program, Texas A&M University.

scholarly journals Raman profiling of embryo culture medium to identify aneuploid and euploid embryos

2019 ◽  
Vol 111 (4) ◽  
pp. 753-762.e1 ◽  
Author(s):  
Bo Liang ◽  
Yuan Gao ◽  
Jiabao Xu ◽  
Yizhi Song ◽  
Liming Xuan ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Culture Medium ◽  
Embryo Culture Medium

80 Evaluation of extracellular vesicles from culture medium of human embryos as a possible method of pre-implantation genetic diagnosis

2020 ◽  
Vol 32 (2) ◽  
pp. 166
Author(s):  
C. Aguilera ◽  
D. Veraguas ◽  
C. Henriquez ◽  
A. Velasquez ◽  
F. O. Castro ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Embryo Culture ◽  
Culture Medium ◽  
Chromosomal Abnormalities ◽  
Genetic Diagnosis ◽  
Poor Quality ◽  
Human Embryos ◽  
Acgh Analysis ◽  
Embryo Culture Medium ◽  
Efficiency Of Selection

Noninvasive methods are the clue to increase the efficiency of invitro-derived embryo selection without decreasing their competence. Embryos selection based on their morphology is the most used method but only 40% of selected embryos are able to implant and develop correctly. In humans, pre-implantation genetic diagnosis increases the efficiency of selection by excluding embryos with chromosomal abnormalities. However, pre-implantation genetic diagnosis needs embryonic cells, which might compromise embryo viability. On the other hand, embryos release extracellular vesicles (EVs: microvesicles and exosomes) to the culture medium that contain biological cargo-like proteins and mRNA lipids, and might contain genomic DNA (gDNA). For this study we evaluated the culture medium from embryos generated by intracytoplasmic sperm injection in a certified fertility clinic. Embryos were cultured in Global Total serum-free medium. The embryos were assessed at Day 3 of development and classified in three categories: top, fair, and poor quality. Corresponding medium was collected for isolation of EVs. The nature of EVs was confirmed by their size and concentration using nanoparticle tracking analysis (NTA), presence of surface markers (CD9, CD63, CD81, and CD40L), and morphology using transmission electron microscopy. A correlation analysis between NTA results (EV size and concentration) and embryo quality was performed. To evaluate chromosomal abnormalities of gDNA present in isolated EVs from embryo culture medium, microarray-based comparative genomic hybridization (aCGH) assay was performed. In a second experiment, aCGH analysis was performed and compared between arrested embryos and EVs isolated from corresponding culture medium. Isolated nanoparticles from embryo culture medium were positive to all markers CD9 (30.9%), CD63 (27.2%), CD81 (31.7%), CD40L (8.7%) and had a morphology accordingly to exosomes. The analysis of NTA data indicated that top-quality embryos had EVs with higher diameter (mean: 112.17nm, mode: 91.74nm) than embryos classified as fair (mean: 108.02nm, mode: 89.67nm) and poor quality (mean: 102.78nm, mode: 88.17 nm; P<0.05). The aCGH analysis showed the representation of the 23 pairs of chromosomes in EVs from culture medium and the chromosomal abnormalities were detected in chromosome 4 (C4: 6/15 (40%)) and chromosome 13 (C13: 6/15 (40%)). In the second experiment, the aCGH assay also showed abnormalities in different chromosomes from samples of EVs from culture medium (24.9%) and were more frequent than those observed in the arrested embryos (8.7%; P=0.03). However, the rate of similitude in chromosomal abnormalities between EVs and their respective embryo was 70-80%. In conclusion, the size and gDNA of EVs from culture medium might be an alternative to evaluate the competence of human embryos. This research was supported by FONDECYT-1170310 and Corfo 17Cote-72437, Chile.

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