scholarly journals Cell-specific translational regulation of S-adenosylmethionine decarboxylase mRNA. Influence of the structure of the 5' transcript leader on regulation by the upstream open reading frame.

1994 ◽  
Vol 269 (27) ◽  
pp. 17905-17910 ◽  
Author(s):  
H. Ruan ◽  
J.R. Hill ◽  
S. Fatemie-Nainie ◽  
D.R. Morris
Keyword(s):  
Translational Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame ◽  
Adenosylmethionine Decarboxylase

scholarly journals Translational regulation of Arabidopsis XIPOTL1 is modulated by phosphocholine levels via the phylogenetically conserved upstream open reading frame 30

2012 ◽  
Vol 63 (14) ◽  
pp. 5203-5221 ◽  
Author(s):  
Fulgencio Alatorre-Cobos ◽  
Alfredo Cruz-Ramírez ◽  
Celine A. Hayden ◽  
Claudia-Anahí Pérez-Torres ◽  
Anne-Laure Chauvin ◽  
...  
Keyword(s):  
Translational Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame

scholarly journals The Nitric Oxide Cycle: Translational Regulation of Argininosuccinate Synthase by an Upstream Open Reading Frame

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Laura Carol Pendleton ◽  
Lindsey R. Jackson ◽  
Sarah L. Yong ◽  
Larry P. Solomonson ◽  
Duane C. Eichler
Keyword(s):  
Nitric Oxide ◽  
Translational Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame ◽  
Argininosuccinate Synthase ◽  
Nitric Oxide Cycle

scholarly journals Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames

2020 ◽  
Author(s):  
Anthony Gaba ◽  
Hongyun Wang ◽  
Trinisia Fortune ◽  
Xiaohui Qu
Keyword(s):  
Single Molecule ◽  
Translational Regulation ◽  
Mrna Translation ◽  
Open Reading Frames ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Translation Efficiency ◽  
Reading Frame ◽  
Reading Frames

Abstract Upstream open reading frame (uORF) translation disrupts scanning 43S flux on mRNA and modulates main open reading frame (mORF) translation efficiency. Current tools, however, have limited access to ribosome dynamics in both upstream and main ORFs of an mRNA. Here, we develop a new two-color in vitro fluorescence assay, Smart-ORF, that monitors individual uORF and mORF translation events in real-time with single-molecule resolution. We demonstrate the utility of Smart-ORF by applying it to uORF-encoded arginine attenuator peptide (AAP)-mediated translational regulation. The method enabled quantification of uORF and mORF initiation efficiencies, 80S dwell time, polysome formation, and the correlation between uORF and mORF translation dynamics. Smart-ORF revealed that AAP-mediated 80S stalling in the uORF stimulates the uORF initiation efficiency and promotes clustering of slower uORF-translating ribosomes. This technology provides a new tool that can reveal previously uncharacterized dynamics of uORF-containing mRNA translation.

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