scholarly journals Sodium-dependent transport of inorganic sulfate by rabbit renal brush-border membrane vesicles. Effects of other ions.

1984 ◽  
Vol 259 (23) ◽  
pp. 14591-14599 ◽  
Author(s):  
E G Schneider ◽  
J C Durham ◽  
B Sacktor
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Renal Brush Border Membrane ◽  
Sodium Dependent ◽  
Dependent Transport ◽  
Inorganic Sulfate ◽  
Renal Brush Border

Sodium-pyrazinoate cotransport in rabbit renal brush border membrane vesicles

1985 ◽  
Vol 249 (3) ◽  
pp. F400-F408 ◽  
Author(s):  
M. Manganel ◽  
F. Roch-Ramel ◽  
H. Murer
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Organic Anion ◽  
Membrane Vesicles ◽  
Sodium Concentration ◽  
Brush Border Membrane Vesicles ◽  
Renal Brush Border Membrane ◽  
Sodium Dependent ◽  
Dependent Transport ◽  
Renal Brush Border

Pyrazinoate (PZA) is an organic anion actively reabsorbed and secreted in the mammalian kidney. In experiments with rabbit renal brush border membrane vesicles, we characterized a sodium-PZA cotransport mechanism that could be involved in reabsorption. An inwardly directed sodium gradient stimulated the influx of PZA. The sodium-dependent transport was electroneutral, suggesting a 1:1 stoichiometry. The kinetic constants for sodium-PZA cotransport were measured under initial linear flux and zero trans conditions for both sodium and PZA. The apparent Km for sodium was about 60 mM. At 90 mM sodium the apparent Km for PZA was about 1.1 mM; increasing the sodium concentration augmented the apparent affinity for PZA. Cis inhibition of sodium-dependent PZA uptake was observed by the addition of nicotinate, lactate, probenecid, succinate, beta-hydroxybutyrate, and salicylate. Urate had no effect. [14C]PZA uptake was trans stimulated by PZA itself, lactate, and nicotinate. PZA shares a transport system(s) involved in the proximal tubular reabsorption of these two anions.

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