scholarly journals Promoter selectivity of Escherichia coli RNA polymerase. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons.

1984 ◽  
Vol 259 (3) ◽  
pp. 1951-1957 ◽  
Author(s):  
M Kajitani ◽  
A Ishihama
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Stringent Control ◽  
Multiple Promoters

scholarly journals Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase.

1987 ◽  
Vol 262 (9) ◽  
pp. 3940-3943
Author(s):  
M. Yamagishi ◽  
J.R. Cole ◽  
M. Nomura ◽  
F.W. Studier ◽  
J.J. Dunn
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Stringent Control

A mutation in the RNA polymerase β′ subunit causing depressed ribosomal RNA synthesis in Escherichia coli

1981 ◽  
Vol 183 (1) ◽  
pp. 54-58 ◽  
Author(s):  
Ben A. Oostra ◽  
Klaas Kok ◽  
Adri J. Van Vliet ◽  
AB Geert ◽  
Max Gruber
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Β Subunit

scholarly journals Regulatory state of ribosomal genes and physiological changes in the concentration of free ribonucleic acid polymerase in Escherichia coli

1975 ◽  
Vol 150 (1) ◽  
pp. 9-12 ◽  
Author(s):  
H Bremer ◽  
D G Dalbow
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Ribosomal Proteins ◽  
Fold Increase ◽  
Ribosomal Genes ◽  
Physiological Changes ◽  
Regulatory State ◽  
Chain Initiation ◽  
State Growth

The concept of promoter efficiency is introduced as frequency of RNA chain initiation at a given promoter normalized to the intracellular concentration of free (but functional) RNA polymerase. Previous observations from this laboratory on the synthesis of ribosomes and β-galactosidase are used to show that during a nutritional shift-up from succinate minimal to glucose-amino acids medium (3-fold increase in steady-state growth rate) the concentration of free (active) RNA polymerase decreases to one-quarter of the pre-shift value and the promoter efficiencies of the genes for ribosomal RNA and ribosomal proteins increase 9- and 6-fold respectively. This extent of control of ribosomal genes is much greater than expected on the basis of the increase in the rate of ribosome synthesis (3-fold).

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