scholarly journals Primary structure of phycocyanin from the unicellular rhodophyte Cyanidium caldarium. I. Complete amino acid sequence of the alpha subunit.

1981 ◽  
Vol 256 (23) ◽  
pp. 12167-12175 ◽  
Author(s):  
G.D. Offner ◽  
A.S. Brown-Mason ◽  
M.M. Ehrhardt ◽  
R.F. Troxler
1982 ◽  
Vol 207 (2) ◽  
pp. 253-260 ◽  
Author(s):  
M A Smith ◽  
L M Gerrie ◽  
B Dunbar ◽  
J E Fothergill

Purification of C4a from heat-activated bovine plasma by elution from CM-Sephadex C-50 at pH 7.4 and gel filtration on Sephadex G-50 gives a 20% yield of pure C4a. The complete amino acid sequence of bovine C4a has been determined by automatic sequencer degradation of CNBr and enzymic fragments, and by carboxypeptidase digestion. The 77-residue bovine sequence shows 12 differences from the human sequence with five of these differences occurring in the C-terminal 11 residues. The sequence of C4a confirms earlier suggestions of homology with C3a and C5a: the three sequences show an almost equal number of identities with each other. The six cysteine residues of the ‘disulphide knot’ are conserved as well as seven other residues including the C-terminal arginine.


1984 ◽  
Vol 144 (1) ◽  
pp. 107-111
Author(s):  
M. Richardson ◽  
F. D. A. P. Campos ◽  
R. M. Moreira ◽  
I. L. Ainouz ◽  
R. Begbie ◽  
...  

1984 ◽  
Vol 144 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Michael RICHARDSON ◽  
Francisco D. A. P. CAMPOS ◽  
Renato A. MOREIRA ◽  
Iracaema L. AINOUZ ◽  
Robert BEGBIE ◽  
...  

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