Characterization of Two Glucuronic Acid-Containing Glycosphingolipids in Larvae of the Green-Bottle Fly, Lucilia caesar

The gene encoding Schizophyllum commune glucuronoyl esterase was identified in the scaffold 17 of the genome, containing two introns of 50 bp and 48 bp, with a transcript sequence of 1179 bp. The gene was synthesized and cloned into Pichia pastoris expression vector pGAPZα to achieve constitutive expression and secretion of the recombinant enzyme in soluble active form. The purified protein was 53 kD with glycosylation and had an acidic pI of 3.7. Activity analysis on several uronic acids and their derivatives suggests that the enzyme recognized only esters of 4-O-methyl-D-glucuronic acid derivatives, even with a 4-nitrophenyl aglycon but did not hydrolyze the ester of D-galacturonic acid. The kinetic values were Km 0.25 mM, Vmax 16.3 μM⋅min−1, and kcat 9.27 s−1 with 4-nitrophenyl 2-O-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylopyranoside as the substrate.

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Characterization of glucuronic acid containing glycolipid in Aspergillus fumigatus mycelium

Carbohydrate Research ◽

10.1016/j.carres.2009.07.012 ◽

2009 ◽

Vol 344 (15) ◽

pp. 1960-1967 ◽

Cited By ~ 15

Author(s):

Thierry Fontaine ◽

Claude Lamarre ◽

Catherine Simenel ◽

Karine Lambou ◽

Bernadette Coddeville ◽

...

Keyword(s):

Aspergillus Fumigatus ◽

Glucuronic Acid

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Characterization of a mucoid Escherichia coli K12 strain, and chemical analysis of the exopolysaccharide

Canadian Journal of Microbiology ◽

10.1139/m72-150 ◽

1972 ◽

Vol 18 (7) ◽

pp. 969-973 ◽

Cited By ~ 2

Author(s):

Stephen Shugar ◽

Kenneth E. Sanderson

Keyword(s):

Escherichia Coli ◽

Chemical Analysis ◽

Glucuronic Acid ◽

Extracellular Polysaccharide ◽

Spontaneous Mutant ◽

Escherichia Coli K12 ◽

Molar Ratios ◽

Genetic Lesion ◽

Long Form

A spontaneous mutant of Escherichia coli K12 C600 was isolated which is mucoid as a result of overproduction of an extracellular polysaccharide. This strain also forms filaments, is sensitive to ultraviolet irradiation, and has its genetic lesion closely linked to the lac gene. Its phenotype and map position are analogous to the lon mutation (long form). The exopolysaccharide contains fucose, glucose, galactose, and may contain glucuronic acid, but the molar ratios of these sugars differ from those previously reported.

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Isolation and Characterization of Glucuronic Acid Conjugates of Chlorpromazine in Human Urine

Experimental Biology and Medicine ◽

10.3181/00379727-102-25331 ◽

1959 ◽

Vol 102 (3) ◽

pp. 602-605 ◽

Cited By ~ 18

Author(s):

T. H. Lin ◽

L. W. Reynolds ◽

I. M. Rondish ◽

E. J. Van Loon

Keyword(s):

Human Urine ◽

Glucuronic Acid ◽

Isolation And Characterization

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The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans

Biochemical Journal ◽

10.1042/bj3160427 ◽

1996 ◽

Vol 316 (2) ◽

pp. 427-437 ◽

Cited By ~ 13

Author(s):

A. J. JAQUES ◽

G. OPDENAKKER ◽

T. W. RADEMACHER ◽

R. A. DWEK ◽

S. E. ZAMZE

Keyword(s):

Cell Line ◽

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Glucuronic Acid ◽

Gel Filtration ◽

Lectin Affinity Chromatography ◽

Tissue Plasminogen ◽

A Cell ◽

Neuroectodermal Origin

The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. We also report the characterization of a series of related and previously unidentified sialylated glycans. Further we show that Bowes t-PA expresses glucuronic acid/sulphate containing N-linked glycans and is recognized by anti-carbohydrate L2/HNK-1 monoclonal antibodies. The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin.

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