scholarly journals Evaluation of carbon flux and substrate selection through alternate pathways involving the citric acid cycle of the heart by 13C NMR spectroscopy.

1988 ◽  
Vol 263 (15) ◽  
pp. 6964-6971
Author(s):  
C R Malloy ◽  
A D Sherry ◽  
F M Jeffrey
Keyword(s):  
Citric Acid ◽  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Carbon Flux ◽  
Citric Acid Cycle ◽  
13C Nmr Spectroscopy ◽  
Substrate Selection ◽  
Acid Cycle

Carbon flux through citric acid cycle pathways in perfused heart by 13 C NMR spectroscopy

FEBS Letters ◽  
1987 ◽  
Vol 212 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Craig R. Malloy ◽  
A.Dean Sherry ◽  
F.Mark H. Jeffrey
Keyword(s):  
Citric Acid ◽  
Nmr Spectroscopy ◽  
Carbon Flux ◽  
Citric Acid Cycle ◽  
Perfused Heart ◽  
Acid Cycle ◽  
C Nmr

scholarly journals Delineation of substrate selection and anaplerosis in tricarboxylic acid cycle of the heart by 13C NMR spectroscopy and mass spectrometry

2010 ◽  
Vol 24 (2) ◽  
pp. 176-187 ◽  
Author(s):  
Wei Li ◽  
Fang Bian ◽  
Priyanjana Chaudhuri ◽  
Xian Mao ◽  
Henri Brunengraber ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Tricarboxylic Acid Cycle ◽  
13C Nmr Spectroscopy ◽  
Tricarboxylic Acid ◽  
Substrate Selection ◽  
Acid Cycle

13C-NMR spectroscopic evaluation of the citric acid cycle flux in conditions of high aspartate transaminase activity in glucose-perfused rat hearts

Biochimie ◽  
1998 ◽  
Vol 80 (12) ◽  
pp. 1013-1024 ◽  
Author(s):  
Son Tran-Dinh ◽  
Jacqueline A. Hoerter ◽  
Philippe Mateo ◽  
Franck Gyppaz ◽  
Martine Herve
Keyword(s):  
Citric Acid ◽  
13C Nmr ◽  
Citric Acid Cycle ◽  
Aspartate Transaminase ◽  
Transaminase Activity ◽  
Acid Cycle ◽  
Rat Hearts ◽  
Perfused Rat Hearts

scholarly journals Metabolism of [3-13C]pyruvate and [3-13C]propionate in normal and ischaemic rat heart in vivo: 1H- and 13C-NMR studies

1995 ◽  
Vol 312 (1) ◽  
pp. 75-81 ◽  
Author(s):  
B Sumegi ◽  
B Podanyi ◽  
P Forgo ◽  
K E Kover
Keyword(s):  
Citric Acid ◽  
13C Nmr ◽  
Rat Heart ◽  
Citric Acid Cycle ◽  
Nmr Studies ◽  
Acetyl Coa ◽  
1H And 13C Nmr ◽  
Acid Cycle ◽  
Propionyl Carnitine

The oxidation of [3-13C]pyruvate and [3-13C]propionate was studied in vivo in infused rats. The infused [3-13C]pyruvate was quickly converted to [3-13C]lactate in the blood, and the [3-13C]lactate formed was well metabolized in both normoxic and ischaemic hearts. Large differences (200-600%) in the 13C enrichment of alanine (C-3) and acetyl-CoA (C-2) compared with lactate (C-3) were found in both normoxic and ischaemic hearts, suggesting that the extracellular [3-13C]lactate preferentially entered a region of the cytoplasm which specifically transfers the labelled pyruvate (formed from [3-13C]lactate) to the mitochondria. The highly enriched mitochondrial pyruvate gave high enrichment in alanine and acetyl-CoA, which was detected by 1H- and 13C-NMR spectroscopy. Ischaemia increased 13C incorporation into the main cytoplasmic lactate pool and decreased 13C incorporation into citric acid cycle intermediates, mainly decreasing the pyruvate anaplerosis. Isoprenaline-induced ischaemia of the heart caused only a slight decrease in pyruvate oxidation. In contrast to the decreased anaplerosis of pyruvate, the anaplerosis of propionate (and propionyl-carnitine) increased significantly in ischaemic hearts, which may contribute to the protective effect of propionyl-carnitine seen in ischaemia. In addition, we found that [3-13C]propionate preferentially labelled aspartate C-3 in rat heart, suggesting incomplete randomization of label in the succinyl-CoA-malate span of the citric acid cycle. These data show that proton observed 13C edited spectroscopic methods, i.e. heteronuclear spin-echo and the one-dimensional heteronuclear multiple quantum coherence sequence, can be successfully used to study heart metabolism in vivo.

EFFECT OF SERUM GONADOTROPHIN ON DEHYDROGENASES OF THE CITRIC ACID CYCLE IN THE OVARY OF THE RAT

1963 ◽  
Vol 42 (4) ◽  
pp. 480-484 ◽  
Author(s):  
B. Eckstein ◽  
R. Landsberg
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Age Groups ◽  
Succinic Dehydrogenase ◽  
Immature Rat ◽  
Acid Cycle ◽  
Immature Rats ◽  
Rat Ovary

ABSTRACT The succinic, malic and isocitric dehydrogenases in the ovary of immature and mature, normal and serum gonadotrophin injected rats were examined. The Qo2 of these enzymes were markedly enhanced in the gonadotrophin injected rats of both age groups, except in the case of succinic dehydrogenase in the ovary of the immature rats, where a slight non-significant decrease was noted. It is concluded that in the mature rat ovary, gonadotrophin administration stimulates the activity of all the examined dehydrogenases of the citric acid cycle, whereas in the immature rat ovary, at least the isocitric- and malic dehydrogenases are thus stimulated.

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