scholarly journals Effects of Vitamin D on Phosphate Transport and Incorporation into Mucosal Constituents of Rat Intestinal Mucosa

1969 ◽  
Vol 244 (1) ◽  
pp. 211-217
Author(s):  
S Kowarski ◽  
D Schachter
Keyword(s):  
Vitamin D ◽  
Intestinal Mucosa ◽  
Phosphate Transport

Tissue distribution and vitamin D dependence of IMCAL in the rat

1987 ◽  
Vol 253 (3) ◽  
pp. G411-G419
Author(s):  
S. Kowarski ◽  
L. A. Cowen ◽  
M. T. Takahashi ◽  
D. Schachter
Keyword(s):  
Vitamin D ◽  
Tissue Distribution ◽  
Intestinal Mucosa ◽  
Plasma Membranes ◽  
Duodenal Mucosa ◽  
Calcium Flux ◽  
Small Intestinal Mucosa ◽  
Calcium Diet ◽  
High Calcium ◽  
Cecal Mucosa

Integral membrane calcium-binding protein (IMCAL) is a vitamin D-dependent integral membrane protein that binds calcium with relatively high affinity (J. Biol. Chem. 225: 10834-10840, 1980). Specific immunoassays for IMCAL utilizing rabbit polyclonal and mouse monoclonal antibodies were developed and applied to studies of its tissue distribution and regulation by vitamin D3 and dietary calcium in the rat. The results indicate that vitamin D-dependent, cross-reactive protein is present in small intestinal mucosa, cecal mucosa, bone, kidney, brain, testis, heart, lung, spleen, and skin. Rats maintained on a low- (0.02%) compared with & high- (2.0%) calcium diet had significantly higher content of IMCAL in duodenal mucosa, cecal mucosa, bone, kidney, brain, testis, and heart. Treatment of rats on the high-calcium diet with 1,25-dihydroxyvitamin D3 increased the IMCAL content of the duodenal mucosa, cecal mucosa, and kidney. The widespread tissue distribution of vitamin D-dependent IMCAL, its close correlation in intestinal mucosa with the calcium transport mechanism, and its occurrence in isolated preparations of enterocyte plasma membranes (microvillus and basolateral membranes) suggest that the protein is involved in the regulation of calcium flux in a number of cell types.

scholarly journals Vitamin D Dependent Calcium Binding Protein in Rat Intestinal Mucosa

1970 ◽  
Vol 16 (3) ◽  
pp. 228-234 ◽  
Author(s):  
KUMIKO OOIZUMI ◽  
SACHIKO MORIUCHI ◽  
NORIMASA HOSOYA
Keyword(s):  
Vitamin D ◽  
Intestinal Mucosa ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein

scholarly journals Regulation of serum 1,25(OH)2Vitamin D3 levels by fibroblast growth factor 23 is mediated by FGF receptors 3 and 4

2011 ◽  
Vol 301 (2) ◽  
pp. F371-F377 ◽  
Author(s):  
Jyothsna Gattineni ◽  
Katherine Twombley ◽  
Regina Goetz ◽  
Moosa Mohammadi ◽  
Michel Baum
Keyword(s):  
Vitamin D ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 23 ◽  
Serum Levels ◽  
Phosphate Transport ◽  
Fibroblast Growth ◽  
Wild Type ◽  
Baseline Serum ◽  
Renal Phosphate Reabsorption

Fibroblast growth factor 23 (FGF23) is a phosphaturic hormone implicated in the pathogenesis of several hypophosphatemic disorders. FGF23 causes hypophosphatemia by decreasing the expression of sodium phosphate cotransporters (NaPi-2a and NaPi-2c) and decreasing serum 1,25(OH)2Vitamin D3 levels. We previously showed that FGFR1 is the predominant receptor for the hypophosphatemic actions of FGF23 by decreasing renal NaPi-2a and 2c expression while the receptors regulating 1,25(OH)2Vitamin D3 levels remained elusive. To determine the FGFRs regulating 1,25(OH)2Vitamin D3 levels, we studied FGFR3−/−FGFR4−/− mice as these mice have shortened life span and are growth retarded similar to FGF23−/− and Klotho−/− mice. Baseline serum 1,25(OH)2Vitamin D3 levels were elevated in the FGFR3−/−FGFR4−/− mice compared with wild-type mice (102.2 ± 14.8 vs. 266.0 ± 34.0 pmol/l; P = 0.001) as were the serum levels of FGF23. Administration of recombinant FGF23 had no effect on serum 1,25(OH)2Vitamin D3 in the FGFR3−/−FGFR4−/− mice (173.4 ± 32.7 vs. 219.7 ± 56.5 pmol/l; vehicle vs. FGF23) while it reduced serum 1,25(OH)2Vitamin D3 levels in wild-type mice. Administration of FGF23 to FGFR3−/−FGFR4−/− mice resulted in a decrease in serum parathyroid hormone (PTH) levels and an increase in serum phosphorus levels mediated by increased renal phosphate reabsorption. These data indicate that FGFR3 and 4 are the receptors that regulate serum 1,25(OH)2Vitamin D3 levels in response to FGF23. In addition, when 1,25(OH)2Vitamin D3 levels are not affected by FGF23, as in FGFR3−/−FGFR4−/− mice, a reduction in PTH can override the effects of FGF23 on renal phosphate transport.

scholarly journals Genetic Variants Associated with Circulating Fibroblast Growth Factor 23

2018 ◽  
Vol 29 (10) ◽  
pp. 2583-2592 ◽  
Author(s):  
Cassianne Robinson-Cohen ◽  
Traci M. Bartz ◽  
Dongbing Lai ◽  
T. Alp Ikizler ◽  
Munro Peacock ◽  
...  
Keyword(s):  
Vitamin D ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Genetic Variants ◽  
Fibroblast Growth Factor 23 ◽  
Phosphate Transport ◽  
Fibroblast Growth ◽  
Vitamin D Metabolism ◽  
Genome Wide ◽  
Fgf23 Concentration

BackgroundFibroblast growth factor 23 (FGF23), a bone-derived hormone that regulates phosphorus and vitamin D metabolism, contributes to the pathogenesis of mineral and bone disorders in CKD and is an emerging cardiovascular risk factor. Central elements of FGF23 regulation remain incompletely understood; genetic variation may help explain interindividual differences.MethodsWe performed a meta-analysis of genome-wide association studies of circulating FGF23 concentrations among 16,624 participants of European ancestry from seven cohort studies, excluding participants with eGFR<30 ml/min per 1.73 m2 to focus on FGF23 under normal conditions. We evaluated the association of single-nucleotide polymorphisms (SNPs) with natural log–transformed FGF23 concentration, adjusted for age, sex, study site, and principal components of ancestry. A second model additionally adjusted for BMI and eGFR.ResultsWe discovered 154 SNPs from five independent regions associated with FGF23 concentration. The SNP with the strongest association, rs17216707 (P=3.0×10−24), lies upstream of CYP24A1, which encodes the primary catabolic enzyme for 1,25-dihydroxyvitamin D and 25-hydroxyvitamin D. Each additional copy of the T allele at this locus is associated with 5% higher FGF23 concentration. Another locus strongly associated with variations in FGF23 concentration is rs11741640, within RGS14 and upstream of SLC34A1 (a gene involved in renal phosphate transport). Additional adjustment for BMI and eGFR did not materially alter the magnitude of these associations. Another top locus (within ABO, the ABO blood group transferase gene) was no longer statistically significant at the genome-wide level.ConclusionsCommon genetic variants located near genes involved in vitamin D metabolism and renal phosphate transport are associated with differences in circulating FGF23 concentrations.

INFLUENCE OF VITAMIN D ON CALCIUM RESORPTION AND ACCRETION

1957 ◽  
Vol 35 (1) ◽  
pp. 1267-1275 ◽  
Author(s):  
B. B. Migicovsky
Keyword(s):  
Vitamin D ◽  
Intestinal Mucosa ◽  
Accretion Rate ◽  
Resorption Rate ◽  
To Come ◽  
Calcium Resorption

The absorption of calcium through the intestinal mucosa of the chick has been shown to come under the influence of vitamin D within 2 to 4 hours after vitamin D administration.A technique is described for the measurement of calcium accretion and resorption rates in the toe bone of a chick. The effect of vitamin D on the rates of accretion and resorption was determined, and it is concluded that vitamin D affects the accretion rate, which in turn could bring about an increase in the resorption rate per bone. It cannot be stated whether the action of the vitamin D on the bone is direct or indirect.

Vitamin D Stimulation of [3H]Orotic Acid Incorporation into Ribonucleic Acid of Rat Intestinal Mucosa*

Biochemistry ◽  
1967 ◽  
Vol 6 (5) ◽  
pp. 1304-1310 ◽  
Author(s):  
Sidney J. Stohs ◽  
J. Elwood Zull ◽  
H. F. DeLuca
Keyword(s):  
Vitamin D ◽  
Intestinal Mucosa ◽  
Ribonucleic Acid ◽  
Orotic Acid ◽  
Acid Incorporation ◽  
Stimulation Of
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