Sensitive liquid chromatographic determination of alkyl-, nitro- and chlorophenols by precolumn derivatization with dansyl chloride, postcolumn photolysis and peroxyoxalate chemiluminescence detection

1991 ◽  
Vol 553 ◽  
pp. 345-356 ◽  
Author(s):  
P.J.M. Kwakman ◽  
D.A. Kamminga ◽  
U.A.Th. Brinkman ◽  
G.J. De Jong
Keyword(s):  
Chromatographic Determination ◽  
Dansyl Chloride ◽  
Precolumn Derivatization ◽  
Chemiluminescence Detection ◽  
Peroxyoxalate Chemiluminescence ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Peroxyoxalate Chemiluminescence Detection

Reversed-Phase Liquid Chromatographic Determination of Hypoglycin A (HG-A) in Canned Ackee Fruit Samples

1994 ◽  
Vol 77 (5) ◽  
pp. 1175-1179 ◽  
Author(s):  
Ghulam Sarwar ◽  
Herbert G Botting
Keyword(s):  
Amino Acids ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Precolumn Derivatization ◽  
Edible Portion ◽  
Fruit Samples ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) method involving precolumn derivatization with phenylisothiocyanate (PITC) was developed for determining levels of hypoglycin A (HG-A) in canned ackee fruit samples. HG-A was extracted by homogenizing the drained fruit in 80% ethanol. By using a Waters Pico-Tag amino acid analysis 15-cm-long column (which is also used for analyzing protein hydrolysates and biological samples) and an LC system, the baseline separation of HG-A from other amino acids was completed in about 6 min. The total time for analysis and equilibration was 16 min. HG-A levels in the edible portion of fruit in 18 cans varied from 18.27 to 87.50 mg HG-A/can. Recoveries of added standard HG-A averaged 101%. To our knowledge, this is the first report of the use of this method to determine HG-A in ackee fruit.

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