Fixation of the central nervous system for electron microscopy by aldehyde perfusion

1965 ◽  
Vol 12 (1-2) ◽  
pp. 187-206 ◽  
Author(s):  
Robert L. Schultz ◽  
Ulf Karlsson
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
The Central Nervous System

scholarly journals Central nervous system virion detection in acute measles: histopathological, ultrastructural and pathogenetic aspects

1995 ◽  
Vol 37 (2) ◽  
pp. 137-143
Author(s):  
C.L.P. Lancellotti ◽  
C.E.P. Corbett ◽  
M.I.S. Duarte
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Clinical Diagnosis ◽  
Clinical Manifestations ◽  
Histopathological Changes ◽  
Ultrastructural Studies ◽  
The Central Nervous System ◽  
Neurological Alterations

Histopathological and ultrastructural studies of 23 patients who died with clinical diagnosis of measles were carried out. In 12 cases viral nucleocapsids were searched by electron microscopy and detected in 100% of the cases in the lungs and in 50% of the cases in the central nervous system. They were mostly intranuclear. Histopathological changes associated to neurological alterations and the detection of virion are discussed in relation to acute and delayed clinical manifestations.

scholarly journals Cerebrospinal Lipodystrophy in Swine

1968 ◽  
Vol 5 (1) ◽  
pp. 67-74 ◽  
Author(s):  
W. Kay Read ◽  
Charles H. Bridges
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Neuronal Inclusions ◽  
The Central Nervous System

Spontaneous lipidosis of the central nervous system of two Yorkshire swine is reported. The presence of membranous cytoplasmic neuronal inclusions similar to those reported in Tay-Sach's disease in man was confirmed by electron microscopy.

Fixation of the Central Nervous System by Perfusion With Aldehydes and its Effect on the Extracellular Space as seen by Electron Microscopy

1967 ◽  
Vol 2 (3) ◽  
pp. 377-386
Author(s):  
PATRICIA V. JOHNSTON ◽  
BETTY I. ROOTS
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Extracellular Space ◽  
Room Temperature ◽  
Lateral Geniculate Body ◽  
Cross Linking ◽  
Apparent Lack ◽  
The Central Nervous System ◽  
Striking Change

Rat brains fixed by perfusion with glutaraldehyde and prepared for electron microscopy under certain conditions showed an abundance of very closely apposed or completely fused surface membranes in some areas. This apparent lack of extracellular space was most consistently observed in the lateral geniculate body and the superior colliculus and less consistently in the cerebellum and parietal cortex. Lowering the temperature of the perfusate from body temperature (38-39 °C) to room temperature (19-20 °C) and/or lowering the pressure at which it was delivered (from 140 to 70 cm of water) led to the appearance of more extracellular space. A striking change in the amount of extracellular space was observed when ethanol was used for dehydrating the samples instead of acetone. When ethanol was employed a space of approximately 100 Å was seen between most cellular elements, whereas acetone dehydration led to an abundance of closely apposed or fused surface membranes. It is suggested that if glutaraldehyde acts by cross-linking proteins in apposing membranes, this reaction is more effectively completed in the presence of acetone and that this may be due to depolymerization of glutaraldehyde by the solvent. It is also suggested that the variation in the amount of extracellular space seen in brains fixed by perfusion with aldehydes may reflect real differences in distances between membranes at the time of fixation.

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