1618: Real-Time Monitoring of Nitric Oxide and Blood Flow During Ischemia-Reperfusion in the Rat Testis

2006 ◽  
Vol 175 (4S) ◽  
pp. 521-521
Author(s):  
Motoaki Saito ◽  
Tomoharu Kono ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Keisuke Satoh
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Real Time ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
Rat Testis

Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis

2006 ◽  
Vol 286 (1-2) ◽  
pp. 139-145 ◽  
Author(s):  
Tomoharu Kono ◽  
Motoaki Saito ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Chiko Shinbori ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Real Time ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
Rat Testis

scholarly journals Real-time monitoring of endogenous lipid peroxidation by exhaled ethylene in patients undergoing cardiac surgery

2014 ◽  
Vol 307 (7) ◽  
pp. L509-L515 ◽  
Author(s):  
Simona M. Cristescu ◽  
Rudolf Kiss ◽  
Sacco te Lintel Hekkert ◽  
Miles Dalby ◽  
Frans J. M. Harren ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cardiac Surgery ◽  
Real Time ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Artery Bypass ◽  
Organ Injury ◽  
Real Time Monitoring ◽  
Myocardial Ischemia And Reperfusion ◽  
Endogenous Lipid

Pulmonary and systemic organ injury produced by oxidative stress including lipid peroxidation is a fundamental tenet of ischemia-reperfusion injury, inflammatory response to cardiac surgery, and cardiopulmonary bypass (CPB) but is not routinely measured in a surgically relevant time frame. To initiate a paradigm shift toward noninvasive and real-time monitoring of endogenous lipid peroxidation, we have explored pulmonary excretion and dynamism of exhaled breath ethylene during cardiac surgery to test the hypothesis that surgical technique and ischemia-reperfusion triggers lipid peroxidation. We have employed laser photoacoustic spectroscopy to measure real-time trace concentrations of ethylene from the patient breath and from the CPB machine. Patients undergoing aortic or mitral valve surgery-requiring CPB ( n = 15) or off-pump coronary artery bypass surgery (OPCAB) ( n = 7) were studied. Skin and tissue incision by diathermy caused striking (>30-fold) increases in exhaled ethylene resulting in elevated levels until CPB. Gaseous ethylene in the CPB circuit was raised upon the establishment of CPB (>10-fold) and decreased over time. Reperfusion of myocardium and lungs did not appear to enhance ethylene levels significantly. During OPCAB surgery, we have observed increased ethylene in 16 of 30 documented reperfusion events associated with coronary and aortic anastomoses. Therefore, novel real-time monitoring of endogenous lipid peroxidation in the intraoperative setting provides unparalleled detail of endogenous and surgery-triggered production of ethylene. Diathermy and unprotected regional myocardial ischemia and reperfusion are the most significant contributors to increased ethylene.

Nitric oxide donors reduce the rise in reperfusion-induced intestinal mucosal permeability

1993 ◽  
Vol 265 (1) ◽  
pp. G189-G195 ◽  
Author(s):  
D. Payne ◽  
P. Kubes
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Ischemia Reperfusion ◽  
Intestinal Blood Flow ◽  
Mucosal Barrier ◽  
Barrier Dysfunction ◽  
Mucosal Permeability ◽  
Cyclic Monophosphate ◽  
Barrier Disruption ◽  
51Cr Edta

Recent data have demonstrated that inhibition of nitric oxide synthesis exacerbated the mucosal injury associated with reperfusion of the postischemic intestine. In this study, using a feline 1-h intestinal ischemia followed by reperfusion model, we tested the possibility that exogenous sources of nitric oxide may prevent the reperfusion-induced mucosal barrier disruption and examined the mechanisms involved. Mucosal barrier integrity was assessed by determining 51Cr-EDTA clearance from blood to lumen. Intestinal blood flow and resistance were also determined. Reperfusion after 1 h of ischemia significantly increased 51Cr-EDTA clearance (0.05 +/- 0.01 to 0.35 +/- 0.07 ml.min-1.100 g-1) and decreased intestinal blood flow by 50%. Exogenous sources of nitric oxide including SIN-1, CAS-754, and nitroprusside as well as exogenous L-arginine all reduced reperfusion-induced mucosal barrier dysfunction without improving intestinal blood flow. Inhibition of endogenous nitric oxide with NG-nitro-L-arginine methyl ester between 1 and 2 h of reperfusion further augmented the rise in mucosal permeability associated with ischemia-reperfusion. Addition of the permeable analogue of guanosine 3',5'-cyclic monophosphate, 8-bromoguanosine 3',5'-cyclic monophosphate, improved reperfusion-induced intestinal blood flow significantly but did not provide protection against mucosal barrier disruption associated with the first hour of ischemia-reperfusion. Exogenous sources of nitric oxide can reduce reperfusion-induced mucosal barrier dysfunction independent of alterations in intestinal blood flow.

Real-time monitoring of peroxynitrite (ONOO−) in the rat brain by developing a ratiometric electrochemical biosensor

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 2150-2157 ◽  
Author(s):  
Feiyue Liu ◽  
Hui Dong ◽  
Yang Tian
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Rat Brain ◽  
Real Time ◽  
Superoxide Anion ◽  
Electrochemical Biosensor ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Real Time Monitoring ◽  
The Brain

As a reactive oxygen species (ROS), peroxynitrite (ONOO−) generated by nitric oxide (NO) and superoxide anion (O2˙−) plays important roles in physiological and pathological processes in the brain.

Real-time monitoring of nitric oxide in ischemic myocardium using an NO-selective electrode calibrated by electron spin resonance

Life Sciences ◽  
2003 ◽  
Vol 74 (1) ◽  
pp. 75-85 ◽  
Author(s):  
Shun-suke Takahashi ◽  
Yoichi Omori ◽  
Hiroyuki Miyazaki ◽  
Fumihiko Yoshino ◽  
Hirofumi Shoji ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Electron Spin Resonance ◽  
Real Time ◽  
Electron Spin ◽  
Ischemic Myocardium ◽  
Real Time Monitoring ◽  
Selective Electrode ◽  
Spin Resonance

scholarly journals A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lina Tang ◽  
Yutao Li ◽  
Hui Xie ◽  
Qing Shu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Real Time Monitoring ◽  
Acupuncture Needle
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