Suitability of the anthrone–sulfuric acid reagent for determining water soluble carbohydrates in soil water extracts

2000 ◽  
Vol 32 (5) ◽  
pp. 725-727 ◽  
Author(s):  
A.S Grandy ◽  
M.S Erich ◽  
G.A Porter
Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2058
Author(s):  
Iván P. Ordóñez ◽  
Ignacio F. López ◽  
Peter D. Kemp ◽  
Daniel J. Donaghy ◽  
Yongmei Zhang ◽  
...  

The increase in drought events due to climate change have enhanced the relevance of species with greater tolerance or avoidance traits to water restriction periods, such as Bromus valdivianus Phil. (B. valdivianus). In southern Chile, B. valdivianus and Lolium perenne L. (L. perenne) coexist; however, the pasture defoliation criterion is based on the physiological growth and development of L. perenne. It is hypothesised that B. valdivianus needs a lower defoliation frequency than L. perenne to enhance its regrowth and energy reserves. Defoliation frequencies tested were based on B. valdivianus leaf stage 2 (LS-2), leaf stage 3 (LS-3), leaf stage 4 (LS-4) and leaf stage 5 (LS-5). The leaf stage development of Lolium perenne was monitored and contrasted with that of B. valdivianus. The study was conducted in a glasshouse and used a randomised complete block design. For Bromus valdivianus, the lamina length, photosynthetic rate, stomatal conductance, tiller number per plant, leaf area, leaf weights, root growth rate, water-soluble carbohydrates (WSCs) and starch were evaluated. Bromus valdivianus maintained six live leaves with three leaves growing simultaneously. When an individual tiller started developing its seventh leaf, senescence began for the second leaf (the first relevant leaf for photosynthesis). Plant herbage mass, the root growth rate and tiller growth were maximised at LS-4 onwards. The highest leaf elongation rate, evaluated through the slope of the lamina elongation curve of a fully expanded leaf, was verified at LS-4. The water-soluble carbohydrates (WSCs) increased at LS-5; however, no statistical differences were found in LS-4. The LS-3 and LS-2 treatments showed a detrimental effect on WSCs and regrowth. The leaf photosynthetic rate and stomatal conductance diminished while the leaf age increased. In conclusion, B. valdivianus is a ‘six-leaf’ species with leaf senescence beginning at LS-4.25. Defoliation at LS-4 and LS-5 was optimum for plant regrowth, maximising the aboveground plant parameters and total WSC accumulation. The LS-4 for B. valdivianus was equivalent to LS-3.5 for L. perenne. No differences related to tiller population in B. valdivianus were found in the different defoliation frequencies.


2019 ◽  
Vol 17 (4) ◽  
pp. 539-544
Author(s):  
Md Juiceball Hassan ◽  
Md Masudul Karim ◽  
Md Amirul Islam ◽  
Md Habibur Rahman Pramanik ◽  
Md Alamgir Hossain

Submergence stress at early vegetative stage is one of the most important constraints in the productivity of rice in Bangladesh. Submergence causes yield loss of rice at Aman season in Bangladesh and therefore, it is necessary to develop submergence tolerant rice cultivars. A pot experiment was conducted at the net house of Department of Crop Botany, Bangladesh Agricultural University, during Aman season from July to December, 2017 to evaluate the changes in root porosity and water soluble carbohydrates (WSCs) associated with submergence tolerance in rice. The experiment consisted of two factors—(i) Rice cultivars (Binadhan-11, Binadhan-12, BRRI dhan51 and BRRI dhan52 as tolerant and BRRI dhan49 as susceptible) and(ii) Submergence stress: Submergence for 14 days at vegetative stage and control. Submergence stress was imposed by dipping of pots into a water tank with about 90 cm depth of water while the control plants are maintained in the pot house of the field laboratory. The plants were sampled at seven days interval during submergence to determine the changes in root porosity and to examine the contribution of shoot reserves for their survival. The root porosity was measured by pycnometer method and water soluble carbohydrate was measured by the anthrone method. Tolerant cultivars showed greater root porosity development in both control and stress condition but the susceptible cultivar showed significantly lower root development in stress condition. Higher root porosity might help tolerant cultivars to survive in submergence stress more efficiently. Tolerant rice cultivars had high initial soluble carbohydrate than the susceptible one. Under submergedcondition, the tolerant cultivars showed slow depletion of water soluble carbohydrate compared to susceptible cultivar. Higher carbohydrate contents in tolerant cultivars might act as buffer stock during submergence for their better survival and growth. J Bangladesh Agril Univ 17(4): 539–544, 2019


2020 ◽  
Vol 206 (5) ◽  
pp. 619-629 ◽  
Author(s):  
Meredith L. Mitchell ◽  
Stephen G. Clark ◽  
Kym L. Butler ◽  
Zhongnan Nie ◽  
Vivianne F. Burnett ◽  
...  

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